XB-CLONE-3053102
Clone Name: IMAGE:7849405
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Gene:
eef2.1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT535119 [+]
caggcgtctgtgtgcagactgaaaccgtgcttcgtcaagccattgcagagcgcatccgccctgtgttgatgatgaacaaaatggatcgtgctcttttgga
gctgcagttggaaccagaggagctctaccaaacttttcagcgcattgtggagaatgttaatgtgattatttccacctacggtgaaggagagactggaccc
atgggaaatatcatgattgaccctgttcttggcacagttggttttggctctggtctgcatggctgggctttcacactaaaacagtttgcagagatgtatg
tcgcaaaatttgcagcaaagggggaaggtcagttggctcctgcagaacgggccaaaaaagtggaggacatgatgaagaagctgtggggtgataggtattt
tgaccctgcaaatggaaagttcagcaagtctgcaacaaacgctgatggcaaaaaacttcctagaaccttctgccagcttgtattggatccaattttcaag
gtatttgatgcaatcatgaattttaagaaagaagaaactgccaaattggtcgagaaactagacatcaaacttgacagtgaggacaaagacaaagagggaa
aacagttgcttaaggctgttatgagacgctggctcccagctggagaggctcttctgcagatgattaccatccacttgccctcaccagtgactgctcagaa
ataccgttgtgagctgttgtatgagggtcccccagatgatgaagctgccatgggtatcaagaactgtgaccccaaaggccctcttatgatgtacatctca
naaatggtgcccacccactgacagggtagattttatgcctttgga
3' EST: DT535118 [+]atttttttaatggcaccaataagtcattcaggttattgcagtacatctttgctctacattctgccctcgttcctgtcaaccctcaaattcacatttactt
ccccagccatccaagatgttttcagagagcagaagctacccatcaagaataaatagaaagttatgatcatgccaatgaaagattatattcagggttccaa
cagaatgttgcagggaatgctgacatcttttgggtatagttcatttccacttaatcaatgctgacaggagtaaagcaaaggtttacagcttgtcaaggaa
gttatccaaggcttgaacaccctctttcaagcccttcctcttccgggtctctgccaccacttggaaaggcctggtggagttatcgaaaggatctccaggt
agaatttgccagtggtcaaacacacactgagggaaggcctggccaccagtgtttgatcttaagtcagcagtgaaaccaaatgactcatttacaggcaggt
atgccttaaccacaaacattggtgttccagccacctgggattcttcaaagacatgcccacgctttctgttcaagacaccatagatacctcctaccacctg
ctcagggcactgaatttcaacaaggtagattggttccatcagacgtggctgagcagtcaacacacaagcatacaagacgcggcgtgcagttggaatgatc
tgtcctcctcctctatggatggcatcagcatgaagtgttacatcatggacatcaaaacgaacacctctcanattttcttcacacaggacaccctcctttg
tagcccactggaatcctgncacaacgctgtctttgatttcattcagatactgaactcccttagtaacatcagtaagtatgttgggcccagagccatcagg
accaaaacaccaaatcttcctagcttcagtcacatcccattcgtac
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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