XB-CLONE-3053060
Clone Name: IMAGE:7849313
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Gene:
chrna1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT535147 [+]
attacatattaatttcattttgtattccacacattcagtttatggataagaatagtcataacctaaacctggtttgcagatagctcttagtataacattt
acagcagcattactctcagtatggcaaatacagtgtaactctcagtatggcaaatacactgttactctcagtatggcatatacactgttactctcagtat
ggcatatacactgttactctcagtatggcatatactgtacactgttactgtcagtatggcatatacactgttactctcagtatggcagatagctgtaaac
aaaaaacatgtgtagttctattctctttaatgggttttacaaagaaaacctttacaatattttgtgttttcttcttcaaccacccaaacattgaaactga
gtaaccaaacaataagagattcatcagctcaaacaacctattacccaatgattattctgtttaaaaaatcataggcaggagtgacataaggacccttgag
ctgtactcttgtgatggtgaaagggccacaggcaaactagcgagtggcatttggacaagagttgtaatggtacatcaaggcttgcaggccaatatgtctg
gagattgaagtttccactaatgcagagcacagcagggcaaaggaatcacttgcagcttttaaatgaaacagatgacagcgaggttccaatttccatggtg
tcctgtaagatttaaccttgcatgtgtaactcaataagccggccagcgaatatagccagcgttccaatgatacacactatcatgaagacagccagcagga
ggtgatctaaaaccattgcaacaaacttccattcttctgaggccttatttgattcctggtcagacttcatggtctctgctatgtatttggc
3' EST: DT535146 [+]tttccctcacgcgtcttattttcctgttcattgcagctactctcgtcttttcttctgaggatgaatcccgccttataaatgacttatttaagagctacaa
caaagtggttcgtccagtcaaggcttttaaggataaggttgtagtgacacttggacttcagctcatacagcttattaatgtggatgaagtcaatcaaatt
gtaacaacaaatgtccgtctgaaacagcaatgggaggatgttcacctaaagtggaacccagaagattatggaggcataaagaaagttcgaatctcttctg
gtgatatttggcgccctgatattgtgctgtacaataatgcagatggagattttgctattgtccaggaaaccaaagtccttcttgattacactgggaaaat
tatatggacgcctcctgcaatttttaaaagttactgcgaaatgattgtcacatactttccatttgacctgcagaactgcagcatgaagctcggtacctgg
acttatgacggctcattagtagttataaatccggagagtgatcgtccagatctgagtaactttatggaaagtggagaatggtacatgaaggattatcgtg
gctggaagcactgggtttattatgactgttgcccagaaacgccatacttggatatcacataccattttctcctgcagaggcttcccctttacttcattgt
caatgtagtgatcccttgtctgttattctcctttctgactggcctggtattttatctgcctactgattcaggagagaaaatcactctgagcgtttctgta
ttgctgtctctggttgtgtttcttctggtcatcgttgaactgattccttctacatcgagcgccgtccctttaattggg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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