XB-CLONE-3052946
Clone Name: IMAGE:7849228
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Gene:
tubb6
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT535223 [+]
ggaaattgtgcatatccaagcaggacagtgcgggaaccagatcggaaccaagttttgggaagtgatcagtgacgagcatggaattgatccagcaggagga
tatgttggtgactcggctctgcagcttgagaggattaacgtgtactacaatgaatcatcttctcagaagtatgtgcccagggcaattctagtggatctgg
aaccaggaaccatggacagtgtgagatcaggacaatttggagagttgttcagaccggacaattttatttttgggcaaactggggcaggaaacaactgggc
aaaaggacattacacagaaggtgcagagcttgttgattctgtactggacattgtcagaaaagagtgtgagcactgtgactgtttgcaagggttccagctt
actcactctcttggaggaggaacgggttctggtatgggaacactaatgataagcaagatacgagaagaatatccagacaggataatgaacacatttagtg
ttatgccgtcccctaaagtctctgacactgttgtggagccttacaatgccaccttgtccgtacatcagctggtcgagaacacagatgaaacttactgcat
tgacaacgaagccctctatgatatttgtttccgtaccctgaaactcactactcctacctatggggatctgaaccacctggtgtctgcaacaatgagtggg
gttaccacatcgttacgatttcctgggcagctcaatgctgaccttcgcaagttggcagtcaatatggtcccatttccacgcctccattttttcatgcctg
gatttgcacctttgactgctcgaggaagtcagcagtatcgagccctcacggtaccagaattaacacaacagatgtttgat
3' EST: DT535222 [+]gctactcattaacttcttcctcatcttcctcaaacgcctcctccccatcattggctgtagcttcttgatactgctggtactctgagacaaggtcgttcat
gttgctttcagcttctgtgaattccatctcatccattccttcccctgtgaaccagtgcaggaaagcctttcttctgaacatggcagagaactgctcagag
atcctcttgaatatctcttggattgctgtactgttgccaatgaaagtggaagccattttcaagccacgcggaggaatgtcacacactgctactttgacat
tgttggggatccattcaacaaagtaactgctatttttgttctgaactgctaacatctgctcatctacttccttcattgacataggacctctgaagactgt
ggctacagtcaaatatcgtccatgacgtgggtcacaagcagccatcatgttcttggcatcaaacatctgttgtgttaattctggtaccgtgagggctcga
tactgctgacttcctcgagcagtcaaaggtgcaaatccaggcatgaaaaaatggaggcgtggaaatgggaccatattgactgccaacttgcgaaggtcag
cattgagctgcccaggaaatcgtaacgatgtggtaaccccactcattgttgcagacaccaggtggttcagatccccataggtaggagtagtgagtttcag
ggtacggaaacaaatatcatagagggcttcgttgtcaatgcagtaagtttcatctgtgttctcgaccagctgatgtacggacaaggtggcattgtaaggc
tccacaacagtgtcagagactttagg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: