XB-CLONE-3052901
Clone Name: IMAGE:7849232
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Gene:
tob1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT535253 [+]
tttcgctgcattcatgtaggagaaaaggtggagcccatcatccagcaggcagccaacgaaagcggcctggaaatcgaggacatccgccggaacctccccc
aggacctcagcgtctggattgacccgtcagaagtgtcctatcagatcggcgagaaggggcagatcaaggttctgtacgtcgacgataactccgagaacgg
cacggaactggataaggagatcaagaacagctttaacccggaggcacaggcgttcatgccaatcaatgaccaatcttcttccgtgtccagctccccgtcg
cccctttttaacgagactgccgccgtcagcccgacgttcatgccgagactcaatcagcctctcaccttcaccaccgctatgttcgctgccaccaagttcg
gttccaccaaaatgaagaacaaccgcaacaagatcgcgcgctcctcccccactcagtttggcctgaacgtcaacctgttgcagcagagcgcgttgtcttc
ctccatgcaccctatgtacggcatcggcctgccgcagaagccctccgccctctcccccaacgcacaggagttcagcttccccagtataccggatccaggc
agacccgccataggctgccccaacgacggaactcttccctacagtaatgccttcgatatgttcgcagcctacggcggcctgaatgataaatcgctggtgg
atggtttgaattacagcctaaacaacatgcagtacagcaaccagcaattccagccggttatggccaactagccgcccaaacacaagtattgaacaagctg
catgagcccaaggttaccattttgtttttttattccttaatctcttcagcttgttgcccttacacatttcatacattccagggttgggtgcataagcaca
t
3' EST: DT535252 [+]ttcatttaaaataattttaatacaaactaaatataaactatctcagctcttaacatgtaagacactgactttaatatactttaacattgtcaagtattgc
acaatttagatacaaaaattacaacatctccatcttttatatagcaaaaatttttttttttttgtttttaaatttgtaacagaaaatacaactctgaaaa
tacatatatcctcggtcatctcttttttttttttttttaaggtttacagcgaggaaaaaaactctaataaactttctgaactggtgtctcaaaaaggaaa
agaaataaaaattacaatcagaaataaaaaaaagtaaaagggagttttgtagctggaatcacagatccatagaaattctgaaatgctttatgtcaatctc
cccatacagggctgggaaactgaatccccgccgccttacgctatttacaataggcgtaatcgcttttcattaaagccctcttctttccagagctgggggc
ggggcaagggggaagggggaggggcctccagtcctgtatgcatttgtaaatataaataatcaacatatcccaatttagaaaaacaaatctcaaggcctat
tatataaaaaaatatataatatgtatttgtagcttctaaatactgtaaggagcaacgttccttataaatgtagtgaacacaagatttgcacttcgctggc
aaaaaaaacaaaaacaaaaaaatggtctatgtgcttatgcaaccaaccttgaatgtattgaatgtgtaagggcaacaagctgaagagattaaggaataaa
aaaacaaaatggtaaccttgggctcatgcagcttgttcaatacttgtgtttgggcggctagttggccataaccggctggaattgctggttgctgtactgc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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