XB-CLONE-3032923
Clone Name: IMAGE:7606627
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Gene:
ist1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC135384 [+]
CACTCCACAACAAAAGAAGCCTTCGGTGAGGTGACAGGGCTGAAGTGTGAGCCGCTTTTGAAGGTAATTAGCTGGGGCTTATCCCGCAGCTACCACATAG
CTAGGAGGGCAGATAAGGGAGCCTTGTACTGGCCCCTGTGTAACTGCGGTGGTGTGAGATCTGCGTGACGCCTTCACATTCGAGCGCCTGCAACCAGGAA
GTTCCATTCAGCCTGACGGCTTGGCTGCGCCTTCAGGGAATTGCGGCTGTGACAGGGAAGAATCACCATGCTGGGCTCTGGTTTTAAGGCTGAACGTCTT
CGAGTCAATTTGAGACTTGTTATAAACCGGTTGAAACTTCTAGAGAAGAAAAAAACTGAATTAGCACAAAAGGCTAGAAAGGAGATTGCTGACTATTTGT
CCACTGGAAAAGATGAGCGAGCACGGATCCGAGTGGAGCACATCATACGTGAAGATTATCTGGTGGAGGCAATGGAGATTCTTGAACTGTACTGTGACTT
GCTTCTGGCACGTTTTGGATTAATTCAGTCTATGAAAGAACTGGATCCTGGCCTGGGAGAAGCAGTTTCTACTCTAATCTGGGCTGCTCCACGCTTGCAA
ACAGAAGTTACTGAGCTTAAAATAGTTGCAGATCAGCTTTGTGCAAAGTACAGCAAAGAGTATGGCAAGCTTTGTAGGACAAACCAAATTGGCACAGTCA
ATGAGCGGCTAATGCACAAGCTGAGTGTAGAGGCTCCTCCAAAAATCCTAGTTGAAAGATATTTGATTGAGATTGCCAAGAATTACAATGTCCCATATGA
ACCGGATGCTGTTGTTATGGCTGAAGTCCCTGCTGGAGTTGAAGCTGACCTGATAGATGTTGGCTTTACAGATGATGGGAGAAAAGGTGGTAGTGGTGGA
GGAGGTGGAGGGTTTGGTGTGCCATTACCAATGGTTTCTGCTATGCAGATGCCAACACCTACCATCCCATTTGCCTACCCTGCACCTGGCGGGCAGGACC
CAGTCAGTGGTGCAACAGTAGGAATCTACCAACCTTTTGGCAACTTAAATCCTCCTCCAATGGGTGGTATGCCACCACCTGTCCCTTCCATGCCGCCTAC
ATATGATACTATTGAGAATTTTGGGCCACAAGCAAATAATCTTCCACCTACAGCTGGTCCCGGTAATAATGTGCCTCCTGTCGCCAAACCAAGAACAAAA
ACTCCCGATAACTTTGTTCTTCCTGAGCTGCCTTCCGTTCCTGACACATTACCAGCTACATCCGTAGGCACAAGCACATCCACCTCAGAAGATATTGACT
TTGACGATCTGTCACGCAGATTTGAAGAACTTAAAAAGAAATCTTAAGGTGGCAGTCATTCTTTCCCACAGGAAGCTTGATACCTGTTCTCAAAAGAAAA
TACTACAGATTTAAAGACTCACATAAAACTGCTGATTGTTGGGCATGAAAGGTGTGTCTTTAAAGAAGAAATGCAGCACTTTTACAGTTATGATTTCAAA
AGGCTTTGTCCTGTTTGACAGTGGCAAGATGGTCAATGATGCATTTGACTGTTAATGATCTTTTATCTAGTTTGATGATCTTGAGGTCTGTGCTGAGAAT
TCTACCTTTTCCTAGAATATCCAGCCCTATCTTTTAACCCACTTGCTCCCACATTTGGCATGGGATTGAGGGATTTGTCTTTCACTCCTAATGAGCTGCT
CAGGCTGCTTGATTTTCTTACTTTGTAAATAACATTTATTAAGTTAGGCTTAATTATGTTTGTGCTCCTTTAAATAAAGTTGTGGCCTTTTCTTCTGCAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CX366988 [+]cactccacaacaaaagaagccttcggtgaggtgacagggctgaagtgtgagccgcttttgaaggtaattagctggggcttatcccgcagctaccacatag
ctaggagggcagataagggagccttgtactggcccctgtgtaactgcggtggtgtgagatctgcgtgacgccttcacattcgagcgcctgcaaccaggaa
gttccattcagcctgacggcttggctgcgccttcagggaattgcggctgtgacagggaagaatcaccatgctgggctctggttttaaggctgaacgtctt
cgagtcaatttgagacttgttataaaccggttgaaacttctagagaagaaaaaaactgaattagcacaaaaggctagaaaggagattgctgactatttgt
ccactggaaaagatgagcgagcacggatccgagtggagcacatcatacgtgaagattatctggtggaggcaatggagattcttgaactgtactgtgactt
gcttctggcacgttttggattaattcagtctatgaaagaactggatcctggcctgggagaagcagtttctactctaatctgggctgctccacgcttgcaa
acagaagttactgagcttaaaatagttgcagatcagctttgtgcaaagtacagcaaagagtatggcaagctttgtaggacaaaccaaattggcacagtca
atgagcggctaatgcacaagctgagtgtagaggctcctccaaaatcctagttgaaagatatttgattgagatttgccagaattacaatgtcccatatgac
ccggatgctgttngtatggctgaagtccctgctggagttgaagctgacctgatagatgttggc
Library:
Description: Library constructed using poly A RNA and oligo dT primers (Invitrogen SuperScript Plasmid System for cDNA Synthesis and Cloning). After second-strand synthesis, cDNA was size-fractionated on a sepharose 4B-CL 30 cm column and directionally cloned into pCS108 (http://mcb.berkeley.edu/labs/harland/pages/plasmids.html). Library constructed by Russell B. Fletcher in R. Harland's laboratory (University of California, Berkeley, Dept of Molecular and Cell Biology).Vector Details | Vector Map |
Name: pCS108
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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