XB-CLONE-3020021
Clone Name: IMAGE:7613883
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Gene:
pdgfra
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC135576 [+]
ATTGAATCAATTACAAGGCACTGGGTCCCAGTCTGTTGCATCTGAGAGGAGAAGGAGACACATCTGCAGGACCCTGGGATTTATACACACATCAACACAC
ACAAACATATTTCATTTTTTCACTTTTAATCTTCTTATTGGGAGTTACTATCGACTTTTACATAGTGGAAAGTTGTTACAGTCCATGATGCCTACCACGA
GGGCATCCCTTGTTTTGGGGTGTCTCCTGATTATAGGTCCGTGGGTCATTCTTGCTCAAAATCCCCTGCCCTCAATATTTCCTGAAAAGGATGAGTTGGT
TCAGAGGTTGCACTCCTCCTTTTCACTAACGTGCACTGGTGAGAGCGAACTGAGCTGGCAGCACCCCCATTCCGAATCTGAACTGGGGAATGTAGTCATT
GGAAGTGAAGAAAACAACAGCGGCCTCTTTGTGTCTGTTCTGGAAGTGACCAACGCTTCCGCCTCCAATACGGGCCTCTACACCTGCTACTACAATCACA
CCCAGACTGAGGACAGTGAGATTGAAGGCACTGATATCTACATCTATGTCCCAGACACCAATGTCCCTTTTGTTCCCCCCGGGTTGTTTGACCATATCAT
TGTGGTTGAGGAAGATGAATCTGCTTTCATACCTTGTCGCACTACCGACCCTTCCAGCCAAGTGACCCTAAAAAGTATCGAAAATTCTAAGACTGTCAGT
GCCTTTTATGACAGCAAGCAAGGATTTGTTGGAAACTTCCAACCAGGATCATACATCTGTGAAACCGTCTCTAACAAAATGGTCTACCAGACAGACCTTT
ACATTCTACAGACATGGAAAGCAACGCACAACATTTCCGTTGAGATGGAAGCCCCCCAAACTGTATTTCGAACTGGGGAAACCATTACCATTTCCTGCAT
TGTGCTGGAGAATGAGGTGGTAGAATTGAAGTGGTACTACCCTGGACAACAGAGAGGCGTTGGCAAAAGAAATGTTGAAGAAAGCAGGGTTCCATCTCAG
AGACTGGTGTACACCTTAACCATTGCAAATGCCACTGCGGATGACTCAGGAGAGTACCAGTGTGCCGTTCTACATGCAACACTAGATAACAAGGTCATCA
AGAAGACAAATATAACAGTTCATGAAAAGGGATTCATCAATCTGGAGCCCATGTTTGGATCTGAGGAATATGCCAACCTCCATGAAGTGAAGAGCTTTAT
AGTAGACTTGCATGCCTATCCCTGCCCTAGGGTCTTCTGGTTGAAGGACAACCGGACACTGAGTGAAAATCTGACAGAGATCACGACTAGCACTGTTACA
ACTAAAGAAACCAGGTTTCAGAGTAAACTTAAACTGATCCGGGCCAAAGAAGAAGACAGCGGACTGTACACTCTTGTGGCACAAAATGACGCAGAAACTA
AAAGCTACTCGTTTATTTTGCAGATAAAAGTACCCGCTTTGATATTGGAACTGGTTGATGAACATCATGGGACATCTGGTGAACAGACTGTTGGGTGCTT
AGCCAAAGGGATGCCGGTCCCTGATGTGGAATGGTTGGTCTGCAAAGACATCAAAAGATGCAACAATGACACCTTGTGGTCCCTTCTGGCAATAAATGGG
TCAGAAATCAGTATGGAGACCCATCAAGATTATGAGAACACAATAGAAAGCCAGGTGACCTTTAAGAAAGTTGAGGAGACAATGGCAATCCGATGTATCG
CAAGAAACGACCTCGGTGTGGTGGCCAGGGAACTAAAGCTGGTTGCACCCACCCTACGTTCTGAACTAACTGTGGCAGCCGCCGTCCTTGTCCTTCTGGT
GATTGTCATCATATCTCTCATTGTACTGGTCATCATATGGAAACAGAAACCAAGGTATGAAATAAGATGGCGAGTGATCGAGTCCATCAGCCCAGATGGC
CATGAATATATCTATGTGGACCCAATGCAGCTTCCTTATGACTCTCGGTGGGAGTTCCAAAGGGATGGACTTGTTCTTGGTCGCATCCTTGGCTCTGGGG
CTTTTGGGAAAGTGGTTGAAGGCACAGCATATGGGTTAAGTCGTTCTCAGCCAGTTATGAAAGTTGCTGTAAAAATGTTGAAACCTACTGCCCGGTCAAG
CGAAAAGCAGGCACTGATGTCTGAACTGAAGATCATGACACACCTTGGCGCACATCTCAATATAGTCAATTTATTAGGAGCCTGTACTAAGTCAGGACCA
ATTTATATCATAACTGAATACTGCTTCTATGGTGACCTCGTGAATTACCTGCATAAGAACCGAGATAATTTTCAGAGCCGACACCCAGAGAAATCAAAGA
AGGATTTGGACATCTTTGGGCTGAACCCAGCTGACGAGAGTACGAGAAGCTATGTTATCTTATCATTTGAAAACAACGGCGACTATATGGACATGAAACA
AGCTGACACCATGCAGTATGTACCCATGCTGGAAATGAAAGAACCCTCCAAGTATTCAGATATCCAGAGATCCTTGTATGACCGGCCTGCGTCGTACAAG
AAGAAGCCTTTGTCAGAAGTGAAAAATATCTTGTCTGATGATGGATTCGATGGATTCGATGGTCTAACAGTACTGGACTTGCTCAGTTTCACATATCAAG
TTGCAAGGGGCATGGAATTCTTAGCCTCTAAAAATTGTGTACATCGGGATTTGGCAGCTCGGAATGTCCTATTAGCACATGGGAAAATTGTAAAAATCTG
TGACTTTGGCCTAGCAAGAGACATCATGCACGACTCCAACTATGTATCCAAAGGCAGCACTTTCCTTCCAGTAAAGTGGATGGCGCCCGAAAGCATCTTC
GATAACCTGTACACAACTCTCAGCGATGTGTGGTCATTTGGAATCCTGCTGTGGGAAATATTTTCTCTTGGGGGCACACCGTATCCTGGCATGATTGTGG
ATTCAACCTTCTATAATAAGATAAAAAGTGGCTACAGAATGGCAAAACCTGACCATGCCACCCATGAAGTGTACGACATCATGGTAAAATGTTGGAACAG
TGAACCAGAAAAAAGGCCATCATTTCGCCATCTGAGTGACATTGTGGAAAGTCTACTTCCTATGGAATACAAAAGGTGCTACGAGACAGTTCTTCATGAT
TTCCTTAAAAGTGACCACCCTGCAGTTACTCGCATGAGAAGTGACAGTGACAATTCCTACATTGGGGTGACGTATAAGAATGAAAACAAAATGAAGGACA
GAGAGAGTGGGTTTGATGAGCAAAGGCTGAGCGCTGACAGCGGTTACATTATTCCCCTTCCTGATATTGATCCCGTTTCAGAAGATGAGTCCAGCAAGAG
GAACAGGCACAGTTCTCAAACATCAGAAGAAAGTGCAATCGAGACCGGGTCCAGCAGTTCCACTTTTATAAAGAGGGAGGACGAGACCATTGAAGACATT
GAGATGATGGACGACATTGGGATCGATTCCTCGGATCTAGTGGAGGACAGTTTCCTGTAAGATCTTGCCGAATCCATTCCACTTGCCAAGCCATTCAAAC
TAAAGGGAAAGGCTGTGAAATCTAGAGATTTATATTTTTTAACTAAAAAAAAAAAAAAAA
5' EST: CX389519 [+]ttgaatcaattacaaggcactgggtcccagtctgttgcatctgagaggagaaggagacacatctgcaggaccnctgggatttatacacacatcaacacac
acaaacatatttcattttttcacttttaatcttcttattgggagttactatcgacttttacatagtggaaagttgttacagtccatgatgcctaccacga
gggcatcccttgttttggggtgtctcctgattataggtccgtgggtcattcttgctcaaaatcccctgccctcaatatttcctgaaaaggatgagttggt
tcagaggttgcactcctccttttcactaacgtgcactggtgagagcgaactgagctggcagcacccccattccgaatctgaactggggaatgtagtcatt
ggaagtgaagaaaacaacagcggcctctttgtgtctgttctggaagtgaccaacgcttccgcctccaatacgggcctctacacctgctactacaatcaca
cccagactgaggacagtgagattgaaggcactgatatctacatctatgtcccagacaccaatgtcccttttgttccccccgggttgtttgaccatatcat
tgtggttgaggaagatgaatctgctttcataccttgtcgcactaccgacccttccagccaagtgaccctaaaaagtatcgaaaattctaagactgtcagt
gccttttatgacagcaagcaaggatttgttgganacttccaaccaggatcatacatctgtgaaaccgtctctaacaaaatggtctaccagacagaccttt
acattctacagacatggaaagcaacgcacaacatttccgttgagatggaagccccccaaactgtatttcgaactggggaaacattaccatttcctgcatt
gtgctgg
3' EST: CX389518 [+]aaaaaatataaatctctagatttcacagcctttccctttagtttgaatggcttggcaagtggaatggattcggcaagatcttacaggaaactgtcctcca
ctagatccgaggaatcgatcccaatgtcgtccatcatctcaatgtcttcaatggtctcgtcctccctctttataaaagtggaactgctggacccggtctc
gattgcactttcttctgatgtttgagaactgtgcctgttcctcttgctggactcatcttctgaaacgggatcaatatcaggaaggggaataatgtaaccg
ctgtcagcgctcagcctttgctcatcaaacccactctctctgtccttcattttgttttcattcttatacgtcaccccaatgtaggaattgtcactgtcac
ttctcatgcgagtaactgcagggtggtcacttttaaggaaatcatgaagaactgtctcgtagcaccttttgtattccataggaagtagactttccacaat
gtcactcagatggcgaaatgatggccttttttctggttcactgttccaacattttaccatgatgtcgtacacttcatgggtggcatggtcaggttttgcc
attctgtagccactttttatcttattatagaaggttgaatccacaatcatgccaggatacggtgtgcccccaagagaaaatatttcccacagcaggattc
caaatgaccacacatcgctgagagttgtgtacaggttatcgaagatgctttcgggcgccatccactttactggaaggaaagtgctgcctttggatacata
gttggagtcgtgcatgatgtctcttgctaggccaaagtcacagatttttacaattttcccatgtgctaataggacatt
Library:
Description: Library constructed using poly A RNA and oligo dT primers (Invitrogen SuperScript Plasmid System for cDNA Synthesis and Cloning). After second-strand synthesis, cDNA was size-fractionated on a sepharose 4B-CL 30 cm column and directionally cloned into pCS108 (http://mcb.berkeley.edu/labs/harland/pages/plasmids.html). Library constructed by Russell B. Fletcher in R. Harland's laboratory (University of California, Berkeley, Dept of Molecular and Cell Biology).Vector Details | Vector Map |
Name: pCS108
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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