XB-CLONE-2986965
Clone Name: IMAGE:7594322
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Gene:
top2a
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX331638 [+]
aaaatagtaattggcattttgcagcttttaatgttaaacttcagtaattgtgctgagtagcatcgtcatggagtgagctgaagtgaacactcggcagggg
agttgtagttgtctgaggcttaagtgcaactttttttttttttttttttttttttttaatttttcatatttctaccaatgattttactgaatcaaagtgc
attttggttttaaatacactggtagtgttatatatgcatttagtcctgatattcttcaataacaaactgtacatgtgtagtgagttttgattgtaaaaaa
agtctgtatatgaatcagtcgggggtgtcccatcctccaatgtcagttgacgacaacacattggctgtacccctttcatcttttttcgccatacatgggc
ttcaggcctgattgctcagatatggtgaaatttggttgcatttgatggttaggttccactgtttgcatgaaagctggcccattgtaattccatttgacta
gtctagcttacacacctttttgattttatttggagaagtacttaagccataagccatagctagttccaacatttctagtggccctataggacaatattgt
tgttattattattaacatttatttataaagcgccaacatattccgcagcgctgtacaataagtgttgtgcataccctaaggtactgcttatatgaaggaa
gtgggtacc
3' EST: CX331637 [+]cctttttttttttttttaaaagggcactatattgaataattttctaattggagtgtgttaagaacaagggactatgttatagtctctgtctgcacagacc
atgagaaatttaacagattttgcatgctaacactaacccaattgttataacccaatataaacgcgaactatctactgaatggtagtgtgttgggggtttc
cttaatggagaaggatctaggggtttttgtagataacaagttgtctaattccaggcagtgtcattttgtggctactaaagcaaataaagtgctgtcttgt
ataaaaaagggcattgactcaagggatgagaacataattttgcctctttataggtccctggtaaggcctcaccttgagtatgcagtgcagttttgggctc
cagtcctaaagaaggatattaatgagctggagagagtgcagagacatgcaactaaactggttaaggggatggaagatttaaactatgaggttagactgtc
gaggttggggttgttttctctggaaaagaggcgcttgcgaggtgacatgattactctgtacaagtacattagaggggattataggcagatagggcatgtt
cttttttcccataaaaacaatcaacgcacccctttagataagaggaacggagcttccatttgaagcagctggtttttcacggtgagggcagtgaggttgt
ggaatgcctttcctagaaatgtggtaatggcagattctgttaatgcctataagaggggcctggatgagttcttgaaacatcagaatatccaaggctattg
tgatactaatatctacagtgtatagattggtaggtgtgggttgtgtgtgct
Library:
Description: Library constructed using poly A RNA and oligo dT primers (Invitrogen SuperScript Plasmid System for cDNA Synthesis and Cloning). After second-strand synthesis, cDNA was size-fractionated on a sepharose 4B-CL 30 cm column and directionally cloned into pCS108 (http://mcb.berkeley.edu/labs/harland/pages/plasmids.html). Library constructed by Russell B. Fletcher in R. Harland's laboratory (University of California, Berkeley, Dept of Molecular and Cell Biology).Vector Details | Vector Map |
Name: pCS108
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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