XB-CLONE-2975031
Clone Name: isl1-HAR418
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Gene:
isl1
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Species: Xenopus tropicalis
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Description: EXRC Clone Number HAR-418. IMAGE:7623033.
Xenopus tropicalis ISL LIM homeobox 1. Full length. Accession number BC135764.
To generate probe for in situ.
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Sequence:
Full length sequence: BC135764 [+]
GCGTCCTGGGGCTAACCTTACGCACTCCATCACTTTTTCATTGTGCCTGGGGATACACCAAGCTGACACAATTTCACTGTGGACATTACACCCTCTTACA
GCTATGGGAGACATGGGAGACCCACCAAAAAAAAAGCGCCTGATTTCCCTGTGTGTTGGATGCGGGAATCAGATCCACGACCAATATATACTGAGGGTTT
CCCCGGACCTGGAGTGGCATGCAGCCTGCCTGAAGTGCGCAGAGTGTAGCCAGTACCTGGATGAGACCTGCACTTGCTTTGTCAGGGATGGCAAAACCTA
CTGTAAAAGGGACTATATCAGGTTGTACGGGATCAAATGCGCCAAATGCAACATCGGCTTCAGTAAGAACGACTTTGTGATGAGGGCTCGGTCCAAGGTG
TATCACATCGAGTGTTTCCGCTGTGTGGCGTGTAGTCGCCAGCTTATCCCGGGGGACGAGTTCGCATTGAGGGAGGATGGCCTCTTTTGTCGGGCAGACC
ACGACGTGGTGGAAAGAGCCAGCCTGGGGGCCAGCGACCCCCTCAGCCCCCTTCACCCAGCAAGACCCCTACAGATGGCAGCAGAACCCATTTCTGCCCG
GCAGCCCGCTCTCAGACCCCATGTACACAAGCAGCCAGAAAAGACCACCAGGGTCCGGACAGTTCTGAATGAGAAGCAGCTTCACACCTTGCGGACCTGC
TACGCTGCCAACCCCCGGCCTGATGCCCTGATGAAGGAGCAGCTGGTAGAAATGACTGGACTCAGTCCCAGGGTCATCAGGGTCTGGTTTCAGAACAAGA
GGTGCAAGGACAAAAAGAGGAGCATCCTGATGAAACAGCTACAACAACAGCAACCCAACGACAAAACCGGATGACAGGGACCCCTATGGTGGCTTCCAGT
CCCGAAAGACACGACGGCGGATTACAAGCGAACCCGGTGGAAGTGCAGACTTATCAGCCCCCCTGGAAGGTCCTCAGTGACTTTGCCCTGCAGAGTGACA
TTGATCAGCCGGCCTTCCAGCAACTGGTTAATTTTTCAGAAGGAGGACCTGGCTCCAACTCTACAGGGAGTGAAGTGGCTTCCATGTCCTCCCAGCTCCC
AGATACCCCAAACAGCATGGTAGCCAGCCCTATAGAGGCATGAGGAGCATGGACTGGTTCCCTCTGTTGAGGGCAAGAACGCAAAGCTTGGACTGAATGG
AAGTATTTAAGGACCCAGCTGATGCAGGCAGAGAATCCAGCCAACTCCATAGGATGCGGGGCCACCTACAGAACACAGGATGGCAGCCACACTGTGAAGA
CAATCATGGGATCTTACTAGGAGTGAATGACAAGGACATGGCGGTTGGAGTATTACAAGAGAACGACAGACAAGAATTGAGTGGTGTAGAGGATTTATAT
CCATGGATCTCAAAAATAGCATTTAACCTTCCCCCCCCCAATACCCCCCTTGGCACATCCCAGAAAAGACCAAGATGGACACAGATAGGAGGGATCACCT
TAATCCGAATGGTGCTGCTTTATCTCTCCCGACATCACTTAATCACTGCCCTGCCAAACAGGAGCTGCTATCAATCTGAAGGCAGTAGCCCAGACTGTAG
CCAAGTTAAAGGGATAGTGGCCAGTGGTGGGCTCCCTGCAAGATGCAATGTGGAGTTGCCATGCAGTGTTTAACCTGGAACTGGGGGCTGAGGAACTCCA
GGTCGCCTATAGCATGCAACAGTTGTTCAGATCCTTTTTGTCCTTTCTCATGAAGTTAAACTAAAACCTTATTAACGTATAATAATGGGGGGATTTAAAC
AAAGACAACAGCTAAAGACACTGCTTATTTTTTTCCAATTTCATTTTTTTTTAATTATTATTATTATTTTTTTTTATGTTCAAACCAAGTCTAGGAAGCA
TTGCAACAACAAGGTATACCTCTCTCTGCCACTTGCGTCTCTGGATTGCGTCTGATTCTTGTCTGTCCGAGGACTTATTATTTTTTTTGTTCCCCCAAAG
ATGTGTATAGTTATTGGTTCAAACGACTGTTCCTCTCTGGAAATAAAGAGAGAAAAAAAGGCAGCATTTTGTTTGCTCTTGCATTGCAAATTATAAAGTA
ATTTATTATTTATTGTCGGAAGACTTGCCACTTTTCATGTTATTTGACTATATTTTTGTTTGCTGAAGTAAAAATATCGAGAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAA
5' EST: CX371826 [+]gcgtcctggggctaaccttacgcactccatcactttttcattgtgcctggggatacaccaagctgacacaatttcactgtggacattacaccctcttaca
gctatgggagacatgggagacccaccaaaaaaaaagcgcctgatttccctgtgtgttggatgcgggaatcagatccacgaccaatatatactgagggttt
ccccggacctggagtggcatgcagcctgcctgaagtgcgcagagtgtagccagtacctggatgagacctgcacttgctttgtcagggatggcaaaaccta
ctgtaaaagggactatatcaggttgtacgggatcaaatgcgccaaatgcaacatcggcttcagtaagaacgactttgtgatgagggctcggtccaaggtg
tatcacatcgagtgtttccgctgtgtggcgtgtagtcgccagcttatcccgggggacgagttcgcattgagggaggatggcctcttttgtcgggcagacc
acgacgtggtggaaagagccagcctgggggccagcgaccccctcagcccccttcacccagcaagacccctacagatggcagcagaacccatttctgcccg
gcagcccgctctcagaccccatgtacacaagcagccagaaaagaccaccagggtccggacagttctgaatgagaagcagcttcacaccttgcggacctgc
tacgctgccaacccccggcctgatgccctgatgaaggagcagctggtagaaatgactggactcagtc
3' EST: CX371825 [+]cccgaaatttttacttcagcaaacaaaaatatagtcaaataacatgaaaagtggcaagtcttccgacaataaataataaattactttataatttgcaatg
caagagcaaacaaaatgctgcctttttttctctctttatttccagagaggaacagtcgtttgaaccaataactatacacatctttgggggaacaaaaaaa
ataataagtcctcggacagacaagaatcagacgcaatccaaagacgcaagtggcaaaaagaggtataccttgttgttgcaatgcttcctagacttggttt
gaacataaaaaaaaataataataataattaaaaaaaaatgaaattggaaaaaaataagcagtgtctttagctgttgtctttgtttaaatccccccattat
tatacgttaataaggttttagtttaacttcatgagaaaggacaaaaaggatctgaacaactgttgcatgctataggcgacctggagttcctcagccccca
gttccaggttaaacactgcatggcaactccacattgcatcttgcagggagcccaccactggccactatccctttaacttggctacagtctgggctactgc
cttcagattgatagcagctcctgtttggcagggcagtgattaagtgatgtcggg
Library:
Description: Library constructed using poly A RNA and oligo dT primers (Invitrogen SuperScript Plasmid System for cDNA Synthesis and Cloning). After second-strand synthesis, cDNA was size-fractionated on a sepharose 4B-CL 30 cm column and directionally cloned into pCS108 (http://mcb.berkeley.edu/labs/harland/pages/plasmids.html). Library constructed by Russell B. Fletcher in R. Harland's laboratory (University of California, Berkeley, Dept of Molecular and Cell Biology).Vector Details | Vector Map |
Name: pCS108
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Whole mount in situ hybridization:
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Suppliers:
Search for clone at:
EXRC