XB-CLONE-2956021
Clone Name: IMAGE:7633138
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Gene:
usp30
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC135925 [+]
GTCAGACGCAGCTGCCTTACAGCATTTGCAGGAGTTGTGACATGTCCTGGGCTCCAGTGAGTACTTGGAGCAGGCGGACACCTTTGGCAGCTTGCTGTAG
TGCCCCAGAGCTCCCTCCTGCTGGAGCCTGGAAAGCTTGTGCTGCTGGTTCTCTTCGAATTGGACCGCAAGGGAGGTGTAAAATGATGAAGAACTGGGGA
ATGATTGGTGGCATTGCTGCTGCTTTAGCTGCTGGAATATATGTTTTATGGGGCCCCATATCGGACAGAAAGAAATACAGGAAAGGTCTTGTACCTGGCC
TGTTAAATTTGGGAAACACCTGTTTTATGAACTCTCTGCTCCAGGGTCTGGCTTCCTGCCCATCTTTCATCAGGTGGCTTGCAGATTTCACGTCAAAGTA
CAGACAGGAGAACAACACTACAGAGCATCAGCACCTTTCTGTCACCTTGCTTCACCTCCTAAAAGCTCTGTGCAACCAGGAGGGTACTGAGGATGAGGTT
TTGGATGCCAGCCCTCTATTGGAAGTCCTCAGGGCTCACAGATGGCAAATCTCTTCCTTTGAAGAGCAGGATGCCCACGAGCTGTTCCATGTGTTAACTT
CGTCATTAGAAGATGAGAGGGATCGCCGTCCTCATGTCACTCATCTTTTCGACCTAGATTCTCTGGAATTTCCATTGGAACCTCAAAGGCAAATCCATTG
CAGGACACAAGTGCCCATCTATCCTATTCCTAGCCAGTGGAAATCTCAGCATCCATTTCATGGGAGACTCACTAGTAACATGGTGTGCAAGCATTGCCAA
CACCAGAGTCCAATGAGATATGACACATTTGACAGCCTCTCTTTGAGTATCCCAGTGGCTACATGGGGTCATCCCATAACGCTAGACCAGTGCTTGCAGC
ATTTCATTTCTACAGAGTCTGTTAAAGATGTTGTTTGTGAAAACTGCACTAAGATCCATGCTGCACAGATACCAAATTCGCAGTCGGTAGAGAATCGCAA
AACAACATTTGTGAAGCAGCTGAAGTTGGGGAAGCTCCCTCAGTGTTTGTGCATCCATTTGCAGCGTCTAAGCTGGTCTAATCAGGGCAGTCCCTTAAAA
CGAAATGAGCACGTACAGTTTAGCGAATTCTTGGCTATGGATCGATTTAAATATCGGATATCTGGATGTTCCACGAGTAAGCAACCTGCTAACCATCTCT
CTGCTGCTGAGCAGGAAACAACAGATGGGAAAGAAGGTGGTGCTCAAAATCCCACAATGCCATTTCTGAATGGAGCCTGTTCTACCTCATATATATCTCC
TCCATTTACAAGCCCACTCCCAACCAACCCTGAATGGACCTCCTCATCATATCTATTTCGCTTGATGGCGGTAGTAGTTCACCATGGGGATATGCACTCT
GGACATTTTGTAACCTACCGGCGTTCTCCTGCTGCCAAGAACCAGAAGCTCACTAGCCAACAATGGCTTTGGATTTCGGATGATACTGTTCGGAGGACAA
ATTTCCAGGAAGTTCTTTCTTCTAGTGCTTATCTGCTTTTCTATGAGCGTATCCAATCCAATCTTCACCATCCAGAAGACCAAAGGGCAGCAGAAAAGTG
AAACCACCACAAGGGAAAAGATTACCCTAAACTTCAGCTTTAAACAAAGCAGGGCAAAGCAGAGACCAGTCTTACCTGACAATGCACGAAAGGGCTTTTT
GTTAACAGCTGGTAATTTGTGCTTTATTCCATATTCTGTGATCTTGATGAAATTTATTGCAGGGTGAAAAGAACAACTAAACATGTTATTTAAAAGTAAT
TCTATTTCATTTTTATTTTTCAAAAGATGAATTAACCACAAGTTTAGACGTCCAAATAGACCAGACTCTGCCTATAATTTTAAACTGAACCCAATTATTG
CCTTAGGGGCAGAAAACCATAATTCATTTCAGCACTTTGTTAACCACTTACTACTTGTAGTGGAATATTCTAGTCAGTGCTAATAAAGGAAAGTACAGAT
AGGGCCCCATAAAAGGTGCATATGTGCTACAAATAATAGCCCTTTGTATCTTTTTTTTAGAGGTAATTATCAAACATATATTGAAATATATTGTCTCACT
GACATATTTTGGCACCTATTTTTAAGCACCCGTAAACACGAACATAAACCATTTGGTGTCAGTAAGGCAATTTTTAATAAATTTAAAAAAGCTGCCACAA
AACAGTTTTGCAATTAATTTGCCATCATAAAAAAATGTATATATGTACATAAGAGATAATTTATTCTTAAAAATTGTATTATGAAATTCTGTAATTGGAG
TTTTAGTTTTCTGATGATGTTAATCTATGGTAATAAAGTTAATTTTGTTAAATAAAAAAAAAAAAAAAAAAA
5' EST: CX374589 [+]gtcagacgcagctgccttacagcatttgcaggagttgtgacatgtcctgggctccagtgagtacttggagcaggcggacacctttggcagcttgctgtag
tgccccagagctccctcctgctggagcctggaaagcttgtgctgctggttctcttcgaattggaccgcaagggaggtgtaaaatgatgaagaactgggga
atgattggtggcattgctgctgctttagctgctggaatatatgttttatggggccccatatcggacagaaagaaatacaggaaaggtcttgtacctggcc
tgttaaatttgggaaacacctgttttatgaactctctgctccagggtctggcttcctgcccatctttcatcaggtggcttgcagatttcacgtcaaagta
cagacaggagaacaacactacagagcatcagcacctttctgtcaccttgcttcacctcctaaaagctctgtgcaaccaggagggtactgaggatgaggtt
ttggatgccagccctctattggaagtcctcagggctcacagatggcaaatctcttcctttgaagagcaggatgcccacgagctgttccatgtgttaactt
cgtcattagaagatgagagggatcgccgtcctcatgtcactcatcttttcgacctagattctctggaatttccattggaacctcaaaggcaaatccattg
caggacacaagtgcccatctatcctattcctagccagtggaaatctcagcatccatttcatgggagactcactagtaacatggtgtgcaagcattgccaa
caccagagtccaatgagatatgacacatttgacagcctctctttgagtatcccagtggctac
3' EST: CX374588 [+]atttaacaaaattaactttattaccatagattaacatcatcagaaaactaaaactccaattacagaatttcataatacaatttttaagaataaattatct
cttatgtacatatatacatttttttatgatggcaaattaattgcaaaactgttttgtggcagcttttttaaatttattaaaaattgccttactgacacca
aatggtttatgttcgtgtttacgggtgcttaaaaataggtgccaaaatatgtcagtgagacaatatatttcaatatatgtttgataattacctctaaaaa
aaagatacaaagggctattatttgtagcacatatgcaccttttatggggccctatctgtactttcctttattagcactgactagaatattccactacaag
tagtaagtggttaacaaagtgctgaaatgaattatggttttctgcccctaaggcaataattgggttcagtttaaaattataggcagagtctggtctattt
ggacgtctaaacttgtggttaattcatcttttgaaaaataaaaatgaaatagaattacttttaaataacatgtttagttgttcttttcaccctgcaataa
atttcatcaagatcacagaatatggaataaagcacaaattaccagctgttaacaaaaagccctttcgtgcattgtcaggtaagactggtctctgctttgc
cctgctttgtttaaagctgaagtttagggtaatcttttccctt
Library:
Description: Library constructed using poly A RNA and oligo dT primers (Invitrogen SuperScript Plasmid System for cDNA Synthesis and Cloning). After second-strand synthesis, cDNA was size-fractionated on a sepharose 4B-CL 30 cm column and directionally cloned into pCS108 (http://mcb.berkeley.edu/labs/harland/pages/plasmids.html). Library constructed by Russell B. Fletcher in R. Harland's laboratory (University of California, Berkeley, Dept of Molecular and Cell Biology).Vector Details | Vector Map |
Name: pCS108
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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