XB-CLONE-2955638
Clone Name: IMAGE:7633479
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Gene:
mapk11
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC135931 [+]
ACAAGATGAGCGGGCGCGTTGGATTCTACAAGCAAGAGCTGAACAAAACGGTGTGGGAGGTGCCGGACCGGTACCAGCGCCTTACCCCTGTTGGTTCCGG
TGCTTTTGGCTCTGTATGCTCTGCATATGATACCAAGACAAGACAAAAGGTGGCAGTAAAAAAGCTCTCCAGGCCATTTCAGTCACTAGTCCATGCAAGG
CGAACGTACAGGGAACTGCGGCTGCTGAAACATATGAAGCATGAGAATGTTATTGGCTTGCTGGATGTTTTCACGCCATCTACTTCAGGAGAAAATTTTA
ATGAAGTGTATCTAGTGACAAATCTAATGGGAGCCGACCTCAATAATATTGTAAAGTGCCAAAAGTTAACAGATGACCATATCCAATTCCTTATATATCA
GTTACTTAGAGGCCTCAAGTATATACACTCTGCTGGAATCATCCATAGAGACTTGAAGCCCAGCAATTTAGCAGTAAATGAGGACTGTGAACTAAGGATT
TTGGCTTGGCACGGCAGGCAGATGATGAAATGACTGGGTATGTTGCAACAAGGTGGTACAGAGCTCCTGAAATCATGTTGAATTGGATGCATTATAATCA
AACAGTTGACATTTGGTCAGTTGGATGCATCATGGCAGAGCTACTGAAAGGCAAAGCTCTCTTTCCAGGAAATGATTACATTGATCAATTAAAAAGGATA
ATGGAAGTAGTCGGAACACCCAACTCAGAATTTCTCCTGAAAATATCATCAGAACATGCCAGAAGATACATTGAATCATTACCTTATATGCCTCAGCAAG
ATCTGAAGGAGGTATTTCATGGTGCCAACCCACTGGCGATTGATCTGCTTGAAAAGATGCTCATTTTAGACAGTGACAAGAGGATAACTGCTACAGAAGC
CCTGGCACATCCTTATTTTGTACAATATCATGACCCAGACGATGAGCCTGAAGCTGAACCTTATGACGAATCAACGGAAAATAAAGAGCGCACTATAGAT
GAATGGAAAGAAATTACATATGAAGAAGTCATTAGCTTTAAATCTCAAGATGTTAAAATTGATAACTTGGAGATTGAACAGTAAGCACAAATTACCTGGA
CAATTCCGCATCCTGGAATTTATGGCTTAACTTGGTTCAGCTGACCAAAGACACAGTCTGCTTTGTAATCAGTTTGTCCCCTGCCTTCAACATGGGGAAG
CCTTGTTTTAATTTATATCATTCTTTTTATGACTGCACCTAAGTTATAGGATAAATTATTAAAGGAGCCATTAGAACCCCTGTAGGAGAGACTTTTTACA
GATCCAGCTTCCAGCGCTGCCTGTACCCATCACAACATGATATTAAAGGCTTGTACTGGTAATCTGTGATAACAATAGAGACCATTTCGATAAATTACAC
CTCTCTGTTTTAGACCAGTCTGAGACATCCAGCATTCGCTTATTCTGCTAAACAGTAATACCAACCGCAGCATCACTCTCCTCACTGAACAGTGCAATTG
CACAAGACACCTGGGGATGTTAAACAAATTTCCTGCCTATCACCTTATTGTGTTCTGTGCCACTTTTCTGCCACTAGTGTGTTATGTGCTTTCCCAGGCT
GTGACACTTTTGTTCCCAAGTGTCAGAAGAGGGAACCGTTTAGGCTGTCACCCACACATTGATTTTTGGTAATTTAATGACATTTCTTTCCATTTTGTTT
TGCTTCGTTGTATATTTAATAATGAATCAAACATGCTATAGTCTGTTGCAGATGTTCCAGAAAATCATAATACTGTGTGTAATATACAATTAACCATAAA
CCCCCACTCCACACACACATCTTTTTACATTGAGATCGATTTATAATAATGACTGAGAAGCCAGGCCACAAGATCATAAAATGAGACACTAAAACAACTT
AAAGGGGCAAGGTGAAGGAGAGAGATGGGATAACAAGGGGTGGAGTCTCAGAAAATGATAAAATTGGTCCCATTCAGCTCAGAATGTTGTCAACTTCACT
TGTCCTTGCTTTTAAAGCGCCCTGATCCATCTTGTAAAATCACTTAATAAGGTTGCATTTGCAGTAAGGACAGGATAGAATGAAACAATTTCTATATCTA
CATATTATAAAATAAAGTACCACAAGATATCAATACCTGTAAATACATTCTTCACAGTGGTTCAGTATTAGGGCGAGTTTCATGTTTTAAATGTTTCATT
GTCATACCATGAATCCTATTGCATGTACAATTACAGAAACTTGTTTCCCAGGGGTGTGCGTGTTCTTGTGCATGTTGTCATTAAAAATACTAAGCCAAAA
GACTGATGAAGGGATCTAAATGTAATGTATACAAAATATGTGCAAAAGATGTCTAGGTGAAAGTGTGCTCTGCAAAATGAGATTGTCTGCCTGCTTATAT
TTATAGCTGGTGCCTTCATGGGGTGAGAGATATGATAATGAGATCAAGCTTTCCAGTTCTGTCTGTTTACAGTTTATCTGGGTGCTGATTTCAGCTAGGA
GACGTCAGCAAATCTTATCTTTGTCCTGCTGCCAATAAAAATGATGCTTTTAAAAAAAAAAAAAAA
5' EST: CX374890 [+]caagatgagcgggcgcgttggattctacaagcaagagctgaacaaaacggtgtgggaggtgccggaccggtaccagcgccttacccctgttggttccggt
gcttttggctctgtatgctctgcatatgataccaagacaagacaaaaggtggcagtaaaaaagctctccaggccatttcagtcactagtccatgcaaggc
gaacgtacagggaactgcggctgctgaaacatatgaagcatgagaatgttattggcttgctggatgttttcacgccatctacttcaggagaaaattttaa
tgaagtgtatctagtgacaaatctaatgggagccgacctcaataatattgtaaagtgccaaaagttaacagatgaccatatccaattccttatatatcag
ttacttagaggcctcaagtatatacactctgctggaatcatccatagagacttgaagcccagcaatttagcagtaaatgaggactgtgaactaaggattt
tggcttggcacggcaggcagatgatgaaatgactgggtatgttgcaacaaggtggtacagagctcctgaaatcatgttgaattggatgcattataatcaa
acagttgacatttggtcagttggatgcatcatggcagagctactgaaaggcaaagctctctttccaggaaatgattacattgatcaattaaaaaggataa
tggaagtagtcggaacacccaactcagaatttctcctgaaaatatcatcagaacatgccagaagatacattgaatcattaccttatatgcctcagcaaga
tctgaaggaggtatttcatggtgccaacccactggcgattgatctgcttgaaaagatgctcattttagacagtga
3' EST: DR901662 [+]tttttttttttttttaaagcatcatttttattggcagcaggacaaagataagatttgctgacgtctcctagctgaaatcagcacccagataaactgtaaa
cagacagaactggaaagcttgatctcattatcatatctctcaccccatgaaggcaccagctataaatataagcaggcagacaatctcattttgcagagca
cactttcacctagacatcttttgcacatattttgtatacattacatttagatcccttcatcagtcttttggcttagtatttttaatgacaacatgcacaa
gaacacgcacacccctgggaaacaagtttctgtaattgtacatgcaataggattcatggtatgacaatgaaacatttaaaacatgaaactcgccctaata
ctgaaccactgtgaagaatgtatttacaggtattgatatcttgtggtactttattttataatatgtagatatagaaattgtttcattctatcctgtcctt
actgcaaatgcaaccttattaagtgattttacaagatggatcagggcgctttaaaagcaaggacaagtgaagttgacaacattctgagctgaatgggacc
aattttatcattttctgagactccaccccttgttatcccatctctctccttcaccttgcccctttaagttgttttagtgtctcattttatgatcttgtgg
cctggcttctcagtcattattataaatcgatctcaatgtaaaaagatgtgtgtgtggagtgngggtttatggttaattgtatattacacacagtattatg
attttctggaacatctgcaacagactatagcatgtttgattcattattaatatacaacgaagc
Library:
Description: Library constructed using poly A RNA and oligo dT primers (Invitrogen SuperScript Plasmid System for cDNA Synthesis and Cloning). After second-strand synthesis, cDNA was size-fractionated on a sepharose 4B-CL 30 cm column and directionally cloned into pCS108 (http://mcb.berkeley.edu/labs/harland/pages/plasmids.html). Library constructed by Russell B. Fletcher in R. Harland's laboratory (University of California, Berkeley, Dept of Molecular and Cell Biology).Vector Details | Vector Map |
Name: pCS108
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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