XB-CLONE-2948660
Clone Name: IMAGE:7637865
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Gene:
smad4
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC135845 [+]
GGCGCGGCCCGTATCTGGGGGTTCTTGTTGTCCGCGGCCTGGGCCGCTGTGGAGCGGGCGGGGGGTGCCGGAAGAAACGGAGCTTTTCTTCACTTCGAAT
TCGGGTTAAACCAAAGATTGGGATTGTAGCGTCTGTGCCCACAGCGTCTCTCCGCGTGCAGAAAGCTGGATCCCGCCGACACCATGTCCATCACAAACAC
CCCCACAAGCAACGACGCTTGCTTGAGCATCGTGCACAGCCTGATGTGCCATCGGCAAGGAGGGGAGAGCGAGACCTTTGCCAAGCGTGCCATCGAGAGC
CTGGTCAAAAAGCTGAAGGAGAAGAAGGATGAACTGGATTCGCTGATCACAGCAATAACGACCAATGGCGCGCACCCCAGTAAATGTGTCACCATTCAGA
GGACGCTCGATGGCCGGCTACAGGTTGCCGGTAGAAAAGGCTTCCCTCATGTCATCTATGCCCGCCTTTGGAGGTGGCCAGACCTTCATAAGAATGAGCT
GAAGCACGTAAAGTACTGCCAGTATGCGTTCGACCTGAAATGTGACAGTGTCTGCGTGAATCCCTACCACTACGAGCGCGTCGTATCCCCTGGAATAGAT
CTTTCAGGACTGACGCTGCAGAGTTCTGCCCCATCCAGCTTGTTGGTGAAGGACGAGTATGGCCATGACTATGCCGAGGGTCAGCAGTCAATTTCTGCAG
GCGACGGACATTCAATTCAGACTATCCAGCACCCACCAAGCAGCAGGGCATCCACTGAGTCCTATAGCAACCCATCCATGCTGGCACCAAGTGAACCCAG
CACACCCAATCCCACCAGTTTCTCCAGCATTCCTGTCCCTTCAACAAGCCAACCGGCGAGTTTACTGGCAGCGACTCACAACGACGGGCTGCTTTCCATC
GCTCCTGTGCCTCCACAAGGGACACAAAATGGCTTCACCGGTCAGCCAGCAACATATCATCATAACAGTACTACAACCTGGACTGGAAGTAGAACAGCAG
CTTACACACCTAATATGTCTCACCATCCGAACGGACATCTTCAGCATCACCCACCCATGCCCCATCCTGGACATTACTGGCCTGTCCATAATGAACTTGC
ATTTCAGCCTCCAATTTCAAATCACCCAGCTCCTGAATACTGGTGCTCCATTGCCTACTTTGAAATGGACGTTCAAGTGGGAGAGACCTTTAAAGTTCCC
TCGAGCTGTCCCATCGTCACTGTTGATGGATACGTGGACCCATCCGGGGGTGATCGGTTTTGCTTAGGTCAGCTTTCCAATGTTCACAGAACAGAGGCCA
TCGAGAGGGCAAGGTTACACATAGGGAAAGGCGTGCAGCTGGAGTGTAAAGGAGAAGGGGACGTTTGGGTTCGCTGCCTCAGTGATCACGCAGTGTTCGT
CCAGAGCTACTACTTGGACAGGGAAGCTGGGAGGGCCCCCGGGGACGCCGTGCACAAAATTTATCCCAGCGCATACATTAAGGTGTTTGATTTACGTCAG
TGTCACCGGCAAATGCAGCAACAAGCCGCAACCGCGCAGGCAGCAGCCGCTGCCCAAGCCGCCGCCGTGGCAGGCAACATCCCAGGACCAGGGTCCGTGG
GGGGAATAGCCCCGGCTATCAGTCTGTCA
5' EST: CX407757 [+]ggcgcggcccgtatctgggggttcttgttgtccgcggcctgggccgctgtggagcgggcggggggtgccggaagaaacggagcttttcttcacttcgaat
tcgggttaaaccaaagattgggattgtagcgtctgtgcccacagcgtctctccgcgtgcagaaagctggatcccgccgacaccatgtccatcacaaacac
ccccacaagcaacgacgcttgcttgagcatcgtgcacagcctgatgtgccatcggcaaggaggggagagcgagacctttgccaagcgtgccatcgagagc
ctggtcaaaaagctgaaggagaagaaggatgaactggattcgctgatcacagcaataacgaccaatggcgcgcaccccagtaaatgtgtcaccattcaga
ggacgctcgatggccggctacaggttgccggtagaaaaggcttccctcatgtcatctatgcccgcctttggaggtggccagaccttcataagaatgagct
gaagcacgtaaagtactgccagtatgcgttcgacctgaaatgtgacagtgtctgcgtgaatccctaccactacgagcgcgtcgtatcccctggaatagat
ctttcaggactgacgctgcagagttctgccccatccagcttgttggtgaaggacgagtatggccatgactatgccgagggtcagcagtcaatttctgcag
gcgacggacattcaattcagactatccagcacccaccaagcagcagggcatccactgagtcctatagcaacccatccatgctggcaccaagtgaacccag
cacacccaatcccaccagtttctccagcattcctgtcccttcaacaag
3' EST: DR903417 [+]tgacagactgatagccggggctattccccccacggaccctggtcctgggatgttgcctgccacggcggcggcttgggcagcggctgctgcctgcgcggtt
gcggcttgttgctgcatttgccggtgacactgacgtaaatcaaacaccttaatgtatgcgctgggataaattttgtgcacggcgtccccgggggccctcc
cagcttccctgtccaagtagtagctctggacgaacactgcgtgatcactgaggcagcgaacccaaacgtccccttctcctttacactccagctgcacgcc
tttccctatgtgtaaccttgccctctcgatggcctctgttctgtgaacattggaaagctgacctaagcaaaaccgatcacccccggatgggtccacgtat
ccatcaacagtgacgatgggacagctcgagggaactttaaaggtctctcccacttgaacgtccatttcaaagtaggcaatggagcaccagtattcaggag
ctgggtgatttgaaattggaggctgaaatgcaagttcattatggacaggccagtaatgtccaggatggggcatgggtgggtgatgctgaagatgtccgtt
cggatggtgagacatattaggtgtgtaagctgctgttctacttccagtccaggttgtagtactgttatgatgatatgttgctggctgaccggtgaagcca
ttttgtgtcccttgtggaggcacaggagcgatggaaagcagcccgtcgttgtgagtcgctgccagtaaactcgccggttggcttgttgaagggacaggaa
tgctggagaaactggtgggattgggtgtgctgngttcacttggtgccagcatggatgggttgctataggactc
Library:
Description: Library constructed using poly A RNA and oligo dT primers (Invitrogen SuperScript Plasmid System for cDNA Synthesis and Cloning). After second-strand synthesis, cDNA was size-fractionated on a sepharose 4B-CL 30 cm column and directionally cloned into pCS108 (http://mcb.berkeley.edu/labs/harland/pages/plasmids.html). Library constructed by Russell B. Fletcher in R. Harland's laboratory (University of California, Berkeley, Dept of Molecular and Cell Biology).Vector Details | Vector Map |
Name: pCS108
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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