XB-CLONE-2946360
Clone Name: IMAGE:7638858
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Gene:
kif18a
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC135921 [+]
GGAGATTTGAATGGAGTCCGCTGCGAATGACAGACACTGCAATCAATGTGCGGACAGGCAGGATGTGAGAAACCCTGAAGAGGAGAGTGGCACAGAATCT
GGGTTATCCAGGGACTTCAAGCAACATTCCCATTTCTGATTTCAGACACACTGTTTAAGCAATTCCTAGACTAGCACTGGATAAATCTGTTCTTTGAAGA
TGGCAGCCTCCCAAGAGGATGTCTGCAGTCATGTAAGGGTGGTGGTCCGAGTTCGACCCGAGAACGAAAAGGAAAAGCAAGGGAACTTTAGCAGAGTTGT
TCAAGTGGTGGACAATCATATCTTGGTGTTCGATCCAAAAGTAGAAGAGGTTGGCTTTTTCCATGGGAGGAGCGCAGCCAATCGAGACATAACCAAAAGA
AAGAACAAGGACCTTAAATTTGTGTTTGATTGTGTCTTCGATGACAGTTCTACTCAACTGGAAGTTTTTGAACAAACAACAAAGATTGTTTTGGAAGGAG
TTCTCAATGGATACAACTGCACAGTTCTGGCTTATGGAGCAACGGGTGCTGGCAAGACTCACACTATGCTGGGGTCACCGGGGGAGCCTGGAGTCATGTA
TCTAACAATGATGGAATTATACAACCGGATTGAGAGTGTAAAAGATGAGAAAGTTTGTAATGTTGCCATTTCTTACCTTGAGGTTTACATTGAGCAGATC
CGAGACCTCCTTTCAAATTCTGGTGCATTGGCTGTGAGAGAGGATGCTCAGAAAGGAGTTGTTGTGCAAGGCTTAACATTACATCAGCCCAAGTCAGCCG
AGGAGATCCTTCAGATGCTCGACTATGGAAACAAAAATAGAACCCAGCACCCTACTGACATGAATGCATCGTCATCACGGTCACATGCTGTGTTTCAGAT
TTACTTAAGACAGCAGGACAAGACGGCAAGTATCAATCAGAATGTTCGAATTGCCAAAATGTGCCTCATTGATCTGGCTGGTTCTGAACGGGCCAGTGCA
ACGAACGCCAAGGGTGAACGTTTACGAGAGGGAACAAACATCAATCGCTCACTGTTGGCTCTGGGGAACGTCATCAATGCTCTAGCAGATCCAAAGAGCA
AGAAACAACATATTCCTTACCGGAACAGTAAACTTACACGTCTTCTGAAAGATTCTCTTGGAGGGAACTGCCGCACTATTATGATTGCCGCTGTTAGTCC
TTCTTCGCTGTCCTACGACGACACTTACAATACGCTCAAGTACGCGAACCGAGCCAAGGACATAAAATCAGCAGTGAAGAGCAACGTAGTCAGCCTGGAC
AGTCACATTAGTCAATATGTGAAGATCTGCGAGCAACAAAAGAAAGAGATTGCGGCATTAAAGGAGAAGTTAAAGGCATATGAAGATCAGAAAGCAATTG
CTCCTGGGAAACCGAAGCAGAATGTTTTGCTTCCTCCATGTCAAAATCAGGCTGAAATTAAGAGATTTCAGGAAATTCTCCGATGTTTGTTTACCAACCG
AGAAGAAATACGAGCAGAGTACCTCAACCTGGAAATGCGTTTCAAAGAAAACGAACTGAAAACGTACTACCAAAAGCAATGCTTTGAGCAAATTCAGATG
CTCTGCTCCCAGGAGAAAGCCGTGAAGGCCTCCTGTAAAAGGGATCACAGGGTAGAGGTGTTGAAAAATCAAAATGTGCTCCTCCAGAAGAAAAAGGAGG
ATGAATTGAAGCGTCTGGAGGAAAACACGACCTGGCTACATCGCCTGGAAAGTGAAATGAAACTATTGAACAATGATGGCAAAATGCCAGAGGAGCTTGG
CAAAGACTTGCAGTGTCACCACTTAACGTTAGAGGTAACAGATTTGAAAACACAAATTAAACACATGACCCGACTTGTGGCTCTTCAGGAAAGGGAAAAC
CGATACAATGAAAAACTTGTTAATGCATTACTGCCATCTTTACGCAGACAGTATCTATTGTTAAGCCAGGTAGGGCTGGCAGATACGTGTGTAGAAGCTG
ACTTTAAGAAAATCGAGCAGTTAGTCCAGAGAGAGAAGGCTGTGGTGTGGGCGGATCAGGCTGAGTCCGATGAAGCAAACAAATGTGATGATTCGGAGAT
TGCTGCTGTTGTCACATTTCCTCATCTGAAATACAGTCAGACAACGCCATGCAAAGAAGAAAAATGTGCGCAGCTTATTCAGTCGGCAGAAAAAATGGCA
CAAAAGGAAAACCCACTAGCAATAGTCAGACATTTCACTGGAGCACAGAATTCACAGACAGAGGAATTTAAAGAAAATGTTCCGCAGAAAGCCATAAATG
TCGTGCCACCTCGGAAGAGGACCAGGCGAAAGTTAATGGATTCTCCCATGTCTACGGAAAGTCATCAGCAAAATGATTCAATCAGCGATGAACTGTCCCC
AATCGTATACACTCCAGAGACCAGTAATAACACAGTGACCCTTCTTAAGCAAGTTGCCAAAATCCCATGTGTCGTAGGAAAAAGGGAGAATAACCCTGGA
CTTCCTGACTATGCCGGCCATGCTGGGTTCAGCTTTCCTATGACAAGCGAATCTAAAGATACGGACATGAACTCAACTGTCATTCTCTCACATGGGGATG
GGGATAAAAGTTCTGAGAACACTGCTGATGCGAAGTCTGGCACGACCAGTATGTTACAAAGACTTGGCCTTTCATCTTTGCTGAGTAAACCTTCTTCTAA
ACCACTGAGTGAGAAACCGTCCTATATGGCAATGACGTCAGCTGCTGAAAGAAAAAGAAAGTTGTTAAGTCTTGCAGGAAACAGTGCAGTGAAAGAAGAT
AGCGTAGCACTTCCCACTGCGAAACGCGTGCGGCAGGATATCTCCCTCGACTGCAAGGCGCTAAGGGTTCAGAGAGTGGCAGCAGGGACTACAAGAAACA
AAGATAATTGCGCTCGGAGACGTCTGCCTAAGAGTGTTTCAGAAAGCGCCTTGGGCGTGAAAGGGAAACCCTTCTCATTTCAAGCGTCCGCCCAGCTTCT
GTTCCAAGGCGGGAAGAAAAGAACAGCCCCTAAAGATTCCTGAGAGCAGAGCTGACTTTCCACATTAGTGTAAAACTGGGGTTTTTGTTAAACAAGAAAA
AGACAAATTCTTGCCATCTCAGTGAACTCCCCCAGTGCTCTGCCCTTTGTCTTCTGCTTTATACTTTTATTTAAACATTAGGTTCATGTGTAGAAGGATG
CAGAAAAATATTCCTAAGGACAGTTTGGCAGTAAAGCATACAGAAACAGAACCATTGCTTGGAATCAAAAACATTTTGACTAGGGTTCCATGGTTTTTCT
CCATGGTCTCCTGAAGGAGCTTCTTGCTCTAGAAACATGAAGGTGTGAGCAACAGTGGGTGCACAAGAAACCATCTTGAACACACAGCAGAAACATAATG
CAGCCTTCTACATTGGCAGTGGCAACTCAAGTGTAATTATAGAAAAAGAGAGTGGATCTGTTATTTCTTCTACTGAGTGACTCTGAGCTTAGGCGTATAC
CCATTAAGCAAGACTTAGTCAATTCTTCCAAAAACCACCAACCATCTTGTGTCAATTGGGCACCATTTAGGAGAATTGCAAAGAGTTTAACTAAAAGCTG
ATTACCCACCTTCACCATGGAATCCTATCCCCAGTGATTCTGTAATACCTTACCTTACTTACAGTTGTCTTAAAAACACAAAGGTACTGATTAGTCCCAC
AAAGATGCAGAGTAGTCCTACAGCCTGGGCCACCTTTCTTCTATCCTGGAACAGAGCTCTCCTACTTCAGCAGTGAACTTATTAAACAATCTATCTTTAA
AAGACTAATGATGTCACGCAAATATGTACAATGGAGCTTTTGTTCAGACTGGGGCCCATAATCACTTGGAGAAGCAAAACTATCTGCAAGCTTTCAAATA
GACAGCTGTGTGCTAGAAAAAGTAAGGAAAACATATATAGTAAATATTTAATAGGTTATAGGTAATGTTAACAGGGGTCAGAAGATCTTGATACCTTATT
CGGGGGGGGGGGGTGTATTCTGTACAAGCTACCCCACACTCTAACATCTATGTTGTAGACTTAATTGTATTCAAGTATGCTCTGTGGATAGTTTATGAAG
CTGACTCCATGCAGGTAGAGTGATGTTCCATAGTGGGTCACACAGTACTGTCATCTGCAGTTTAATAACACATAGGTGACCGATTTGTACTGTTTCTACA
CTTTGGCCTTTTTGTGGCAGATGGGACATATACTACTTTCCTGTTGACTAGTTACAACCCGCACATGATTGGGGGGGAGATATTCCACCAGTGGAGTTAT
CACTTTTGATCAAGTGTATATAGTTTTCAACTGAGCTGAGCCTAGGCCTATACCCATTGAGCAAGACCCATTTTTCCAATAACCACCAACCATCTTGTGC
CAAATGTGGGCACCATTCATGAGAGCTGCAAAGCGTTTAACTGGAAGTTGATTACCCCCATCCCCTACCATGGAATCCTACCCCAGTTGATCTGCAACAC
CTACTTACAGTTGTCTTAAAAACACAAACATACCAAAATATACCTTATGCTACACAGCCCCAAGGAGATCCTTCTGGATATGGAGTCCATAAAACATTTC
AGCACCATGAAGTATATATTGAAATATATATTTATTATGCCTCAGTGGCACAATTGGCCAATTTCTATTTAAACTGGCACCTTGCATTTTTTTTATGCCA
TTTTTAAATAAGTGCTGATTGGTTGACTGCTTAGCCAGTCAGCAGTTGGCTTAAGGGCATATTGCACCAGCATCAAATCCCCTTCATTCTCAATAATAAG
TCTTAATATGTAGCCTTTTTTTCCGCTTTTTAATTTATTTTATCCGACAGCAGTGTGATTTTCCTTTTACTGCAAATGCATTTGGTTCTGTTGGAGGCAT
GGCCGCACTCTCTTATTGGCAAGCAGGAGTTCCCCACACGTTGTGGTAAAGAGAAAAGAATGAATTTACAGCCGGCAACCCCAGCACCGACCTAAAAAAG
AAAATCTACGTCTTTGAGTTTCCCGTAAGCTTTGGTTTGTGCTAATGGTTTCTGTGAATGATCGTTATGAATGATCTTTTATTTTTACATTGTAAATATT
GTTTTCCTGTGTTAATATATTTTCCATTTGGACTATAAATGCGTCACATCTTGTAAAAAAAAAAAAAAA
5' EST: CX384643 [+]ggagatttgaatggagtccgctgcgaatgacagacactgcaatcaatgtgcggacaggcaggatgtgagaaaccctgaagaggagagtggcacagaatct
gggttatccagggacttcaagcaacattcccatttctgatttcagacacactgtttaagcaattcctagactagcactggataaatctgttctttgaaga
tggcagcctcccaagaggatgtctgcagtcatgtaagggtggtggtccgagttcgacccgagaacgaaaaggaaaagcaagggaactttagcagagttgt
tcaagtggtggacaatcatatcttggtgttcgatccaaaagtagaagaggttggctttttccatgggaggagcgcagccaatcgagacataaccaaaaga
aagaacaaggaccttaaatttgtgtttgattgtgtcttcgatgacagttctactcaactggaagtttttgaacaaacaacaaagattgttttggaaggag
ttctcaatggatacaactgcacagttctggcttatggagcaacgggtgctggcaagactcacactatgctggggtcaccgggggagcctggagtcatgta
tctaacaatgatggaattatacaaccggattgagagtgtaaaagatgagaaagtttgtaatgttgccatttcttaccttgaggtttacattgagcagatc
cgagacctcctttcaaattctggtgcattggctgtgagagaggatgctcagaaaggagttgttgtgcaaggcttaacattacatcagcccnagtca
3' EST: DR903716 [+]tttttttttttttttacaagatgtgacgcatttatagtccaaatggaaaatatattaacacaggaaaacaatatttacaatgtaaaaataaaagatcatt
cataacgatcattcacagaaaccattagcacaaaccaaagcttacgggaaactcaaagacgtagattttcttttttaggtcggtgctggggttgccggct
gtaaattcattcttttctctttaccacaacgtgtggggaactcctgcttgccaataagagagtgcggccatgcctccaacagaaccaaatgcatttgcag
taaaaggaaaatcacactgctgtcggataaaataaattaaaaagcggaaaaaaaggctacatattaagacttattattgagaatgaaggggatttgatgc
tggtgcaatatgcccttaagccaactgctgactggctaagcagtcaaccaatcagcacttatttaaaaatggcataaaaaaaatgcaaggtgccagttta
aatagaaattggccaattgtgccactgaggcataataaatatatatttcaatatatacttcatggtgctgaaatgttttatggactccatatccagaagg
atctccttggggctgtgtagcataaggtatattttggtatgtttgtgtttttaagacaactgtaagtaggtgttgcagatcaactggggtaggattccat
ggtaggggatgggggtaatcaacttccagttaaacgctttgcagctctcatgaatggtgcccacatttggcacaagatggttggtggttattggaaaaat
gggtc
Library:
Description: Library constructed using poly A RNA and oligo dT primers (Invitrogen SuperScript Plasmid System for cDNA Synthesis and Cloning). After second-strand synthesis, cDNA was size-fractionated on a sepharose 4B-CL 30 cm column and directionally cloned into pCS108 (http://mcb.berkeley.edu/labs/harland/pages/plasmids.html). Library constructed by Russell B. Fletcher in R. Harland's laboratory (University of California, Berkeley, Dept of Molecular and Cell Biology).Vector Details | Vector Map |
Name: pCS108
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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