XB-CLONE-2945210
Clone Name: IMAGE:7639679
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Gene:
mcm8
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC135954 [+]
GGAGGCGGCTGGAAGGAGCCGGTTGCAGTTTTTGTGCTTTCCATGACATTTTCTAATGAGTCAGGTCTGGCGAGGAGGATGGAGTGGGGGAAGAGGAGGC
AACCCATATGCAGGAGGTTGGAGAGGTCGGCCATGGAGAGGAAGGGGCCAGGGTGGATCCTGGAGCAGAAATAGTGGAAGAGATCCTGTTTGTTTTTCAA
CTGCTCCCCCTAAGCCTCAGCTAACACAGACTACACTAGATAAATACATTCCCTACAAAGGCTGGAAGCTTTATTTTTCTGAAGCTTACAGCGACAGCTC
CCCATTTTTGGAAAAGGTTCGTGCCTTTGAGAAGTTTTTTACCAAACAAATTGAACTTTATGACAAGGATGAAATAGAAAGGAAAGGAAGTATTCTTGTG
GATTACAAAGACCTCCTGCAAGATGAAGACCTTTCTGCATCCATCCCAATGTCCTCAGAGCTTAAAGAGATGCCTGAGAAAATCCTGGAGTGCATGGGTC
TAGCTATACATCAGGTGTTAACTAAAGATCTTGAGAGGCATGCAGCAGAACTGCAGGAGCAGGAGGGCCTGAGGACAGAGGAAGCACCTATAGTTAATGT
GCCCTTTATTCATGCCAGGGTGTTTAATTATGATCCACTGACACCTCTAAAGAATCTCAGGGCAAGTTTGTACGGCAAATATGTTGCTTTGCGTGGAACA
GTTGTTCGTGTCAGTAACATCAAGCCGTTGTGCGTTAAAATGGCTTTTTCCTGCAACATGTGTGGTGACATTCAGAGTTTTCCTCTTCCTGATGGAAAGT
ATGCTGTGCCTACAAAGTGCCCTGTGCCAGAATGTCGGGGGCGCTCATTTACTGCCAACAGAAGTTCCCCTTTAACAGTAACTGTGGACTGGCAAACCAT
CAAAGTCCAGGAGCTAATGTCAGATGATCAGCGAGAGGCAGGTCGAATTCCAAGAACAGTTGAGTGTGAACTAATTCAGGATCTGGTAGACAGCTGTGTT
CCTGGCGACATGGTGACTGTAACAGGCATTGTCAAAGTATCAAATACAAGAGACGGTGGCTTTAAGAACAAAAATAACAAATGCATGTTCCTTCTCTATA
TCGAGGCAAATTCTGTCAGTAACAGCAAAGGACAAAAAATCAAAAGTACCGATGACAGCGAGAGTCATGGAGCCTCTATGGATTTTTCTCTGAAAGACCT
CTATGCTATTCAGGAAATTCAAGCCCAAGAAAATCTGTTTCAATTAATAGTCAACTCACTTTGCCCTACAATATATGGACATGAGGCCGTGTGCAATGTA
GCTCCTCGTGGAGTTTATGTTTGTGGAAACACTACCACCACCTCCGGACTGACCGTGACTTTATCCAGAGACAGCGCAACTGGAGATTTTGGCTTGGAAG
CTGGGGCACTCATTCTTGGAGATCAAGGTATATGTGGAATAGATGAGTTTGATAAGATGGGCAACCAGCACCAGGCGTTACTGGAAGCAATGGAACAGCA
AAGCATTAGTTTGGCTAAAGCTGGCATTGTCTGCAGCTTGCCAGCCAGGACATCTATCATTGCCGCTGCAAACCCTGTAGGGGGACACTATAACAAAGGA
AAAACCGTTTCTGAAAATTTAAAAATGGGGAGTGCCTTACTTTCTCGTTTTGACTTAGTGTTCATCCTTCTTGATACCCCGAATGAAGACCATGATCACT
TGCTTTCAGAGCATGTGATGGCTATGAGAGCCGGGGCTAAGGAAATGCAGAGTGCGGATCTGACGTGTCCAACTACACAGAACTCCAACACCTCTGTTCT
AGAGGAGCCCTCTGAAAGGCCCTTAGGAGAAAGACTAAAGCTGAGGCCTGGGGAACACTTTGATCCTATACCACACCAGCTGTTAAGAAAATATGTTGGA
TACGCTCGGCAATATGTCCACCCAACTCTGTCTCCAGATGCAGCCCAGGTTCTTCAGGACTTCTATCTTGAATTAAGAAAACAAAACCAAGGCATAGATA
GTACTCCCATTACCACAAGGCAGCTGGAGTCTCTTATCAGACTGACAGAGGTACAGAAGACAGGGTTATTGGTTAATGCGTAAATCAGGTATTTAATGGT
TTAAATCTTAAAGGACAAGTCAACCCAAAATATAATTTTTTGCCTAATAAAATAAAACATAATTCTAAGCAACTTTGCAATATACATTCATTACATATTT
TTGACTTATTTCTATATATTTCTTGCTTTTAGAAGTAGTGTTTGCCTGCCCTTTTCTATTCCCTGCCCTGGTGGCTCGGGCCCAGCAGACTGACAGACCT
GTCTTGCTGGGGGAGACTGACCTTTGCAACATTGTTTAAAAAGTTACAACCGGGAGTTCAGCAATTGCTGCTTTCAATAGCAATTACATGATTGAATTAT
TTTTTCTTTTATTAGGCAAAAACTTATTTTGGGGGTTGACATGTCCTTTAATTGGCGCTACCTTTCGATTCCTTTTATTGTCTCCAAGTTTTAAATTCTA
CCCAGGGTCAAGCATTATTCTAGGCAGAAAAAAATTATTTGGGTTTTATAGACATGTATTTCCTGGGAAACTATCAAAACGCTGATTCTTCATACATGTT
AATTGGGGAGGTATCTCAAAGGAAATGAGACTTGGGCCCCTAGTGGAGATGCAATATATTCACATATATGTTACATAGTTGTACAGCTGTAATTATCCAT
CATCCTAATCACAGCGTGAATGTGTGAGTATCAGCTTCATTGATTTGTGGTTTCTGCTAAGGCACGGGCGAGACTGGAATTAAGAGAGAAGGCTACTAAA
GATGATGCTGAAGAAGTTGTTCAGATAATGAAGTACAGCCTGCTGGGCACGTTCTCTGATGAGTTTGGAAAGCTGGACTTCCATCGATCTCAGCATGGTT
CAGGAATGAGTAATCGGTCGAAAGCGAAAAAGTTCATATCTGCTCTGAATAGGATTGCCGAACAAACGTACAACAATCTGTTTGAGTTTCAGCAACTTCG
CCAAATTGCCAAAGAGCTACAAATCCAGGTTATTGACTTTGAAGGTTTTGTAGGCTCCTTAAATGATCAAGGCTATCTTCTGAAGAAAGGCCCCAGAGTC
TATCAGCTCCAGACTATGTGACTTTCCCAACATGTTGACACCAAAATGCCTTTATTGTGTTCAAAGAAATCACAGCAGAACTGCCACAAACCCATTACTC
ACTTTCCAACGTCAGGAAACAGTTAATGTTTATTTCATTTGGATTGTTCTGACCAAAACCATTTCCCCCCAGCAAATCTGCTGACTTTATGCACCTTGTA
ATGCTATGTACTTTGTAAGCAAATACTTCCAGTATGGTTCTGCAGCTGAAAGAAATGGACCCAGAAGTGTTACATAGTCACTAAATGTACAGTTAAACTG
TATTAAAATGTTTATTTTCACTGTAGTAAAAGAATGTTTTACAATTCTGAAAAGCCTGTCATCATTTATGTACTGTACATACAAGATAACTTTTAGTATA
TTATAAAAAATCCTGTTCTTAGCAACTTATTTCAGTTGCCCTTCCTGTTTAAGTTATTGTTTTTTTACTTATTTGCCTTTCTCCATTTGGTCACTGAGTG
AGGCTCACTCTGTGAGGCTGCAATTTTATTTGTTATTGTTACTTTTAATTACTTATCTTTCTATTCAGACCCTCTCCTTTTCATTTTATTCCCTCATCTA
AACCACTGCCTACATGTAGTTGCTGGGTAAATTCAACCATAGTGACAAGATGGCTACTGAAATTCCAAACTTGAGAGCTGCTCCAGGAAACCAACTAAGG
CCTAAGGGAAGCGTTTCAGTGCTCATCACCACATGGGACTGTGCATAATAGGAATCTGGACAAGCTGAATATCACTAAATCGTTATATGTTTATTTGGCT
GAACTGCTCTGATCCATTTGTTAAACTGACAGGTTGAATGTGTGGACATAATAAAGCTTGTTCTCAATGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CX385509 [+]ggaggcggctggaaggagccggttgcagtttttgtgctttccatgacattttctaatgagtcaggtctggcgaggaggatggagtgggggaagaggaggc
aacccatatgcaggaggttggagaggtcggccatggagaggaaggggccagggtggatcctggagcagaaatagtggaagagatcctgtttgtttttcaa
ctgctccccctaagcctcagctaacacagactacactagataaatacattccctacaaaggctggaagctttatttttctgaagcttacagcgacagctc
cccatttttggaaaaggttcgtgcctttgagaagttttttaccaaacaaattgaactttatgacaaggatgaaatagaaaggaaaggaagtattcttgtg
gattacaaagacctcctgcaagatgaagacctttctgcatccatcccaatgtcctcagagcttaaagagatgcctgagaaaatcctggagtgcatgggtc
tagctatacatcaggtgttaactaaagatcttgagaggcatgcagcagaactgcaggagcaggagggcctgaggacagaggaagcacctatagttaatgt
gccctttattcatgccagggtgtttaattatgatccactgacacctctaaagaatctcagggcaagtttgtacggcaaatatgttgctttgcgtggaaca
gttgttcgtgtcagtaacatcaagccgttgtgcgttaaaatggctttttccctgcacatgtgtggtgacattcagagtttttctcttcctgatgngaagt
atgctgtgcctacaaagtgccctgtgc
Library:
Description: Library constructed using poly A RNA and oligo dT primers (Invitrogen SuperScript Plasmid System for cDNA Synthesis and Cloning). After second-strand synthesis, cDNA was size-fractionated on a sepharose 4B-CL 30 cm column and directionally cloned into pCS108 (http://mcb.berkeley.edu/labs/harland/pages/plasmids.html). Library constructed by Russell B. Fletcher in R. Harland's laboratory (University of California, Berkeley, Dept of Molecular and Cell Biology).Vector Details | Vector Map |
Name: pCS108
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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