XB-CLONE-2930365
Clone Name: CAAO6948
|
Gene:
supt20h
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX940105 [+]
cctaataacacagtgcgtccatgatgcatgcatgggaagttgtgctggcctgtatgcacaatatggagcccttctgtcccattcagtgtcccaccccccc
tcatactggaacttttcactctgaggaattacatgccacaggcaagtggcaagttccaaaaacatttctttgcacgtgtgtttgggaatgtatttgccag
atgttaatgcagctttttaattctatgtaaaatggcccaataaacacaagatatagaacacagtcattggctttttatacattccagcagcaattaatgc
tttgtttttcctgtttcacagtggtcctcagacactgatgagtggctcaaatccaatattggggtgtaacactggtgccattacccctgcagggataaat
ctgagtggcattttaccatcaggaagtctagtgccaggggcattgccggctgccatgcagtctgcctcacaggcaggtggcccgtttggcttaaaaaata
cttcaggtctgaggcctttaaatctgctgcagctttctggtgctcccatcctattcaaccccatgcagcagcagcagcagcagcaagtctcccagttttc
acctcaacagccccaacagacatcaacttccagcccacagcagcagggagaacagctgggactgttaccagcgggaatggaccaaggttctagcaaccaa
gatcagcaggcagctgtaattaacttaaccggagtaggcggcttcatgcctccccaagcagctgtgttgtctcagcttggctctgctgggaacagacccg
ggcaaaacctgccatcacagagactgcagctctctcctgcctttacacaacagcagcagcagcaacaaaatacattgcaattcttgcatcatcaaatggc
tatgggcacagcagca
3' EST: CX940104 [+]taaggggggggcccccctttgggaaaaaaaaaaaaaaaccggggggccttttaccccccaaaggttgggccctaggggggggggggcccacccccccccc
ccaggggggggggggaaaaaaacttcccccccccccccccgggggggggggggccccccaaccccaaaaaagggggggcccttttaggggccaaaaaagg
aaaaaaccccccccaaaaagggggggggaaaaattcggggaaaaaaaatttaaaaaggttttaaaaaaaaaaaaatggggggggaaaaacggggaaaaaa
ttccccccaaaggggggaaagggggttcaagggccgggggtttggggaaaaacgggggcggcccccccttttaattcttaaaggggggccaaaaaaaggg
ggggggggaaaagggggcgcttgggggggggttgcccaacccctttgggggggaaaaaaatgcaattgtttttttggcccccccccccggtggttaaagg
gaggaaaaaacccccccccccgggaggggggggtttgcccggggtttttccc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: