XB-CLONE-2926011
|
Clone Name: CAAO9408
|
Gene:
tuba1c
|
Species: Xenopus tropicalis
|
|
|
|
Sequence:
5' EST: CX957750 
[+]
ccgagactccttgctgtcggtcggtgagaagccgcaagctgtgctttcccattccgtaaaactatctgctgtctaacagaccccatccgagtcagtcatg
cgtgagtgcatctctatacacgttggccaggctggtgtgcagatcggcaatgcctgctgggaactgtattgtttggaacatggcatccaacctgatgggc
agatgcccagtgacaagaccattggaggaggagacgactccttcaacacctttttcagtgaaactggagcaggaaagcatgtccccagggctgtctttgt
agacttggaacccactgtcattgatgaggtgcgcactggcacatataggcagttattccacccagagcagttaattacaggaaaggaagatgctgccaat
aattatgcccgggggcactacacaattggcaaggagatcattgatctggtgctagacaggattcgcaaactggctgaccagtgcacaggtctccagggtt
tcctggtcttccacagctttggtggtggtactggttctggattcacctccctgctgatggagcgtctctctgttgattatggaaagaagtctaagctaga
atttgccatctatccagctcctcaggtctccacagctgtggttgagccctacaactctattctcacaacccacaccaccctggagcactctgactgtgct
ttcatggtagacaatgaggccatttatgacatctgccgcaggaatctagatattgaacgcccatcctacaccaacctgaacaggctcattagtcagatag
tgtcttctatcacagcctctctgaggtttgatggagccctcaatgtagatctcacagagtttc
3' EST: CX957749 [+]acactttaaagccacatttaaaatggcgctttattttaaaaaaaaaagttagacttcagaaggggaaattcaggaatagaaaggcacaacttgaggaaaa
cagaataaagtttaatactcttcgccttcatcttctgcatctgcactatctgcaccaacctcttcataatctttctccagggcagccatgtcttcacggg
cctcagagaactcaccctcctccataccctcacccacataccagtgcacgaaggcacgcttggcatacatgagatcaaacttgtggtcc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te4 libraries). Library constructed at the DOE Joint Genome Institute.| Anatomy: testis | Stage: frog |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
Usage:
Suppliers:
