XB-CLONE-2925572
Clone Name: CAAO10411
|
Gene:
hspa2
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX959611 [+]
cgcgtccgatcagagattgcatgagtgatttaggttcttttccttaagttaaggtttacatacttggtacagtagaattataattccattttgtagtttc
catggtttggaaaaaacttaattacaaataacagttatgccataaatgagactagtagactagttataggccttcgcctagtcacttagaaaacatgcac
ttgtcttgcagtctggtcattctcactgactagatttagttttgcctgacagctctagccaagattatacataagtaatattgctactgtgaaataaaaa
attaataatataagtcaactaatatgaaactaatatgaaaggaggctgaatatcagttaaggtgatacctaagccttatagcagcacttaattgcaaccc
ccccccccgaacatacaaaagggttgttatgtctatgcagtatctacaccttcaaaccaagattatataaatccctgctctttatttgatgttttatatt
gatatacaacagctacatctataacaatgtttacattacaactgttgacttcctgtctatattagtgagatgaacaaaaggcagcctctgcagtcatttg
tcattggcccttaaaatatatacagctatataatatacaagtgtaggtataaacaagtaaattatatacctgtatcttatagaaatttcacctgcatatg
catgcacagtcacatactgcccttctctgatttgctttttttaatcagaaatattgtgagtggtgtaagcaaaaagctactttaaatagattact
3' EST: CX959610 [+]aggtaaacattttattgaaagcttatctagaaatcagtttaatttgaagtcaaaatatacaaatgtataacattaaaagcaaatttgttatttttcaaag
taacataagtatgtactgaatgatagtgttagtgaacttctttatcacaaaagcactatgggcatttctaattgtgttcctgatctgtggcctctgtgtt
cgttccaccactcagaattaacaccctaaacaaagataaacacgccatgttaaactgcctgcagcaattcagtgtcccaataaagaagaataaggctagt
ttagttactttgttactgtgtggggcaaagacaaaaaaagtttaaaacacccataaaacttttttcataacatagcttttgttatgctagtaaactccag
ttctgcagcaacaaatactctaaagaacgtgttggtgagagagtctataagctagatactgtgcataatatgctatttgtccattgcatcagatgtagta
ctcagtatgtagaatcacaatttggcctgaacagtaatctatttaaagtagctttttgcttacaccactcacaatatttctgatttaaaaaaagcaaatc
agagaagggcagtatgtgactgtgcatgcatatgcaggtgaaatttctataagatacaggtatataatttacttgtttatacctacacttgtatattata
tagctgtatatattttaagggccaatgacaaatgactgcagaggctgccttttgttcatctcactaatatagacaggaagtcaacagttgtaa
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: