XB-CLONE-2899333
Clone Name: CAAO9008
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Gene:
actg1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX957015 [+]
gcagcggacttgacagctactccccgnctctttgtaacaacttgccagctttgcatttctcctcttaaggaaacctccagcaccaagaaataaaatggat
gaggaaattgcagcactcgtcgttgacaatggctctggaatgtgcaaagccggctttgctggagatgatgctccccgtgctgtgttcccctctattgttg
gacgcccaagacaccagggtgtaatggttggtatgggccagaaggacagctatgtaggtgatgaagcccaaagcaagaggggtatccttacccttaagta
tccaattgaacacggaattgttacaaactgggatgatatggagaagatttggcatcacaccttctacaatgagcttcgtgtagcccctgaagagcaccca
gtcctgcttacagaagctcccctgaaccccaaagccaacagagaaaagatgacacagatcatgtttgagactttcaacaccccagctatgtatgtcgcca
ttcaagctgtgctgtctctgtatgcctctggtcgtaccactggtatcgtgatggactctggagatggtgtcacccacacagtacccatttatgaaggtta
tgccttgccccatgctatcctgcgtctggacttggctggtcgtgacctgacagactacctcatgaaaatcctgaccgaaagaggctacagcttcaccacc
acagctgagagagaaattgtgcgtgacatcaaggagaaactctgctatngtgctcttgactttgaacaagagatggcaactgctgcatcctcctcatcct
tggaaaagagctacgaactgcctgatggacaagtaattacaattggaaatg
3' EST: CX957014 [+]actgcagcgctttattttatccttacagaacggtgttttggaagttacaattctcatctggatgacatacaaattttggttttctacgttgtgagaacat
taaggcaacatacaaaaaaatttacataaattaacttggatgcataaatttacaggtgttaactgcaacatttgtaactttcttcagacccattttgaat
gttgggaatacaacctgggttaagaaaaagactggttcttgggcctgagtgtcaatttccaaccttactataaactggaaagatagaagagattccttca
tgaactgaccagcctccgacatgaaacaatggagtattaagcgcttaactgtgtgtggaagtctaaacctcagataccctgtacacatctccaaaactcg
acttctccataaacagcccctgtaacctagaggagaagtgtcattcgcaatactgtcgcagatcggcagcctttcacagaaatgcttctttctacgtaaa
gcttagcttaagtgtcacccagactgtatgccaaccaggcaatataagagatgtgtacagggtacaatatgctagcattgatgccacaggaatgatttga
agagacaagcacaagtcttgcattacaaaatcccccaacagtgactatgtgtgtctccaattcctaggataagttcatttggaagttggccctttttcca
gtttcaagaggctagcatgaggcgtacacatttgccaaggcaaattaatactttgaagaactcacgcagcaaatgcaacgcatctgctagcagtcc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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