XB-CLONE-2872295
Clone Name: CABK6376
|
Gene:
tuba1c
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: DT446257 [+]
cctctctgaggtttgatggagccctcaatgtagatctcacagagtttcagaccaacttggtgccctatccccgtattcacttccctcttgccacctatgc
ccctgtcatctctgcagagaaggcttatcatgagcagcttactgtagctgacattacaaatgcctgctttgaaccagctaaccagatggtgaaatgtgat
ccacgccacggtaaatacatggcttgttgcctgttgtaccgtggtgatgtggtacccaaagatgtaaatgcagctattgccaccatcaagacaaagcgta
ccatccagtttgtggattggtgcccaactggtttcaaagttggtatcaactatcagcccccaactgtggttccagggggagacctggccaaggtgcagcg
tgctgtgtgtatgctgagcaataccactgcgattgctgaggcctgggctcgtctggaccacaagtttgatctcatgtatgccaagcgtgccttcgtgcac
tggtatgtgggtgagggtatggaggagggtgagttctctgaggcccgtgaagacatggctgccctggagaaagattatgaagaggttggtgcagatagtg
cagatgcagaagatgaaggcgaagagtattaaactttattctgttttcctcaagttgtgcctttctattcctgaatttccccttctgaagtctaactttt
ttttttaaaataaagcgccattttaaatgtggctttaaa
3' EST: DT446256 [+]acactttaaagccacatttaaaatggcgctttattttaaaaaaaaaagttagacttcagaaggggaaattcaggaatagaaaggcacaacttgaggaaaa
cagaataaagtttaatactcttcgccttcatcttctgcatctgcactatctgcaccaacctcttcataatctttctccagggcagccatgtcttcacggg
cctcagagaactcaccctcctccataccctcacccacataccagtgcacgaaggcacgcttggcatacatgagatcaaacttgtggtccagacgagccca
ggcctcagcaatcgcagtggtattgctcagcatacacacagcacgctgcaccttggccaggtctccccctggaaccacagttgggggctgatagttgata
ccaactttgaaaccagttgggcaccaatccacaaactggatggtacgctttgtcttgatggtggcaatagctgcatttacatctttgggtaccacatcac
cacggtacaacaggcaacaagccatgtatttaccgtggcgtggatcacatttcaccatctggttagctggttcaaagcaggcatttgtaatgtcagctac
agtaagctgctcatgataagccttctctgcagagatgacaggggcataggtggcaagagggaagtgaatacggggatagggcaccaagttggtctgaaac
tctgtgagatctacattgagggctccatcaaacctcagagagg
Library:
Description: The library was prepared from total RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1610 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).Anatomy: spleen | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: