XB-CLONE-2716972
Clone Name: xtbs15D06
|
Gene:
actb
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CN078960 [+]
gggagtgacccgcccgcacagaaacaggacagtgtgtgtgtcgtactctcagattacaatggacgacgatattgctgcactggtcgttgacaatggttct
ggtatgtgcaaagccggctttgcaggtgatgatgcaccccgtgctgttttcccatccattgttggtcgcccaagacatcagggtgtcatggttggaatgg
gacagaaagatagctacgtaggagacgaagctcaaagcaaaagaggtattcttacactcaaatatcctattgagcatggcattgtcactaactgggatga
tatggagaagatctggcatcataccttctacaatgaactcagagtggccccagaggaacacccagtgctgctgacagaagcacccttgaatcccaaagct
aaccgggagaaaatgacgcagatcatgtttgagacctttaacactccagctatgtatgttgccatccaagctgtgttgtccctgtatgcatctggtcgta
ccactggtattgtcatggactcaggtgatggtgtaacacacactgtgccaatatatgaaggttatgctctaccccatgccattctgcgtctggacttggc
tggacgtgacctgacagactacctcatgaaaatcctcactgagagggggtacagcttcaccaccacagctgaaag
3' EST: CN078959 [+]tggttttatttttttagttaggttacaggtgacccgtcacatcttatgtgatcaatgataatgtaaaccttccaggtgttagagaatgatgccctttttt
gggttaaacaagttacaagtgttcaaggcagaagatacagagacatgtttgggtacatagctgcacaagaaaaaaaaacaaaaaaaaaaaaaaatcaaaa
aacacaatagtatcaccatgatttctgtatgagcttcccaccaagctgggcaacattgaaagggtagcttcctcaatacaatactattgactgaataaaa
tcataatgtcctctgcattgatgaccatacagtgctatttgttcattccagttatcaaatctgagccaagcgccaatgaaagaaaagccatgccaatgtt
atcttcctgcacaggtacatttgttcacgttgaaagggtccgtccttttagaagcatttacgatgcactattgagggcccagactcatcatactcctgct
tgctgatccacatctgctggaaggtggacagggaagccaagatggaaccgccaatccagacagagtatttacgctcagggggagcaattatcttgatttt
catggtgctgggagctagtgcagttatttctttctgcattctatcagcaattccagggtacattgtggttccaccagagagaacagtattggcatagagg
tccttacggatatctacatcacacttcataattg
Library:
Description: Xenopus tropicalis polyA+ RNA was obtained from brain and spinal cord of tadpoles at stages 58-61 and 62-64. cDNAs were synthesized using the SMART system of CLONTECH and directionally cloned into pCMVSPORT6_xtbs, a modified version of pCMVSPORT6 allowing directional cloning using asymetric SfiI sites. For antisense RNA synthesis, use T7 promoter and for sense RNA use SP6 promoter. Library constructed by Dr. L. Coen and Prof. B. Demeneix (Museum National d'Histoire Naturelle and CNRS UMR 5166, Paris, France).Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SfiI
3' Restriction Site: SfiI | |
Usage:
Suppliers: