XB-CLONE-2715014
Clone Name: xtbs17P03
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Gene:
ankef1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CN080239 [+]
gggggatggaacagtagggaaggaagaatttgcctctgcccttctggaggcaggagctcccataactccggagcagcttcagaatattctaggtcgtcac
gagaaaggacgaacagggaccctaagtccagaggaatttttgaaaggttccaagtatctgcaaaaggcatttttactctcttcctatggcctgaaaaaga
aaaagggcaagaagaaaggcaagaaaggaaaaaagggaaaactttccttgcccatgcctatatgtacaatgccaagtgacttcatttcacgccgcgatga
tggcggccctcccaaatttctaatcccaacttttcaaaatgtcacagacttaaatcgtttcgactgggatcaccctccccagaatcctctaactgacgac
accggctggtacattgatgagccggagaaagtgtatacccacatcagtgtcgccaccaagactggagatatggaatccctgaggaaagcatttgaagatg
gcgtgcctctggatgtcaaggatatgttctataaaactccacttatgtctgcttgtgcttcaggcaacctgaatgttgtccaatatcttcttgaacaagg
gtaaaaactacagtccctttcaagtgctgaagaaaataatgatggtgctgaggcct
3' EST: CN080238 [+]ttggcttgtatatgtctatcagccggtagtcagcataagcctttgcaacatctaaaactgtctgaccttttttgttctgtatttgtacttttgcaccaga
atttattagtaaatgagcacagttcaaactcccactttcaattgccctcattaatggtgtggatccgttgaaagcaagggcgtcaatcttggcaccgtgt
ttaagcagcaggtcagctatggcgtcttggctggcgtgacaggcatggtgcaaaggcgtccacaaaaaattatcggttgcattgacgtcagcactggaaa
atacaaagggagcattaaccagagttctctgcacttttctaaaagagagactttttagctggcattttttttatgaattagtctatatttataaagcatt
atcaggcccagaatggtagtctggggattctggctaacaccagaggggctgctgtaagatgccatagaaagtcactatttattgggcttgtggggggctg
tttggggctctgtgtacttgaaatgccagggcctatttagaatcccagtccaggcctcagcaccatcattattttcttcagcacttgaaagggactgtag
tttttacccttgttcaagaagatattggacaacattcaggttgcctgaagcacaagcagacataagtggagttttatagaacatatccttgacatccaga
ggcacgccatcttcaaatgctttcctc
Library:
Description: Xenopus tropicalis polyA+ RNA was obtained from brain and spinal cord of tadpoles at stages 58-61 and 62-64. cDNAs were synthesized using the SMART system of CLONTECH and directionally cloned into pCMVSPORT6_xtbs, a modified version of pCMVSPORT6 allowing directional cloning using asymetric SfiI sites. For antisense RNA synthesis, use T7 promoter and for sense RNA use SP6 promoter. Library constructed by Dr. L. Coen and Prof. B. Demeneix (Museum National d'Histoire Naturelle and CNRS UMR 5166, Paris, France).Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SfiI
3' Restriction Site: SfiI | |
Usage:
Suppliers: