XB-CLONE-2710216
Clone Name: xtbs22D17
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Gene:
actb
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Species: Xenopus tropicalis
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Sequence:
5' EST: CN083330 [+]
ggagagggggtacagcttcaccaccacagctgaaagagaaattgttcgtgatatcaaggagaaattgtgctatgttgctctggactttgagcaggagatg
gccacagctgcttcttcttcatcattggaaaagagttatgagctgcctgatggacaagttatcaccattggtaatgagcggtttagatgtccagaagcac
tcttccagccttctttccttggtatggaatcctgtggcattcatgaaaccacttacaactcaattatgaagtgtgatgtagatatccgtaaggacctcta
tgccaatactgttctctctggtggaaccacaatgtaccctggaattgctgatagaatgcagaaagaaataactgcactagctcccagcaccatgaaaatc
aagataattgctccccctgagcgtaaatactctgtctggattggcggttccatcttggcttccctgtccaccttccagcagatgtggatcagcaagcagg
agtatgatgagtctgggccctcaatagtgcatcgtaaatgcttctaaaaggacggaccctttcaacgtgaacaaatgtacctgtgcaggaagataacatt
ggcatggcttttctttcattggcgcttggctcagatttgataactggaatgaacaaatagcactgt
3' EST: CN083329 [+]tggttttatttttttattttaggttacaggtgacccgtcacatcttatgtgatcaatgataatgtaaaccttccaggtgttagagaatgatgcccttttt
tgggttaaacaagttacaagtgttcaaggcagaagatacagagacatgtttgggtacatagctgcacaagaaaaaaaaaaaacaaaaaaaaaaaatcaaa
aaacacaatagtatcaccatgatttctgtatgagcttcccaccaagctgggcaacattgaaagggtagcttcctcaatacaatactattgactgaataaa
atcataatgccctctgcattgatgaccatacagtgctatttgttcattccagttatcaaatctgagccaagcgccaatgaaagaaaagccatgccaatgt
tatcttcctgcacaggtacatttgttcacgttgaaagggtccgtccttttagaagcatttacgatgcactattgagggcccagactcatcatactcctgc
ttgctgatccacatctgctggaaggtggacagggaagccaagatggaaccgccaatccagacagagtatttacgctcagggggagcaattatcttgattt
tcatggtgctgggagctagtgcagttatttctttctgcattctatcagcaattccagggtacattgtggttccaccagagagaacagtattggcataaag
gtccttacggatat
Library:
Description: Xenopus tropicalis polyA+ RNA was obtained from brain and spinal cord of tadpoles at stages 58-61 and 62-64. cDNAs were synthesized using the SMART system of CLONTECH and directionally cloned into pCMVSPORT6_xtbs, a modified version of pCMVSPORT6 allowing directional cloning using asymetric SfiI sites. For antisense RNA synthesis, use T7 promoter and for sense RNA use SP6 promoter. Library constructed by Dr. L. Coen and Prof. B. Demeneix (Museum National d'Histoire Naturelle and CNRS UMR 5166, Paris, France).Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SfiI
3' Restriction Site: SfiI | |
Usage:
Suppliers: