XB-CLONE-2629527
Clone Name: IMAGE:8863183
|
Gene:
lmna
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC159282 [+]
agtactttcggtttgggatataggtaaacaggtgattttaaggtctaaattgtgattcagtgcgtttttagaatcctgcaatgtgtgactaataggagcc
ctaaagaaagactatatacagtacaacggatcaaatcagctacttgtgaaccgtcaaaacgccagacttacagtaatgttagctcagtttcctctcagct
tgcagccgcattgagagctatcgctggtcgaaaaggcgatatttagcacataactaatctgaaggtagtatttttattatttttttttttcactcggatc
aactcacggtttcatcatcgtcaacatataagacgcaactttcgtgttttcacgaacaaaactgtcttatgaacgacaaatgaagtgaaaaaaaaaaaca
agaatgggtcagtcgacaagcggacaaccgcatgtttcttctatctatgagagatcagagtttcggtccagatcagacacattctcaggtgtgatgccag
atgttcctctagctgtggttgaggagatcttgctgtacctgcccgctcatcaggtggtgcgagtgtgtcgtctggtgtgtcctgagtggaaagagctggt
ggacagtgctgcacactggagagagcgctgtcggagagaggggattcagccctgtgatgcttccagaccgccagaagactggtgtcagttttactttata
actaaaaaacgacggaacctgatcaagaatcccaaagctgaagatgagttcaatgattggatgattgtacagaatggtggtgatctgtggacgattgaag
ataatggtgtcgaaattccaaaacacacagtgtctaaatactttgtcacctcttactggttatgtttaaagcaacagctgattgatttgcagaaagaagg
ctacagtgctgctttcatggatcatctgcaacctcatatcaaaatctcagactggtatggcccacgcattgattgtgggtgtgagtatcagatctgtgtg
gagttgcttgatcagaagaagaaacccatcagtacctttgaacccgaaccagttatctttccacagtggagtgactggcagtggtgtgaaatgattcatg
tcttcaaggattatggacctggtgttcggtttatccgtttcactcacggagggaaggatactcagttctgggcaggtcattatggaataagagtaactaa
cagtagtgtggagatctgcccagctgcagagagagagtcattgtctgtttgagtatttcctctgttataagaaacactatgaaataatggacctgcttaa
attattatttttattattattatttaatatttttgaccatttattgaccattacactgttataaagtaatctaagcatgcaatgtaaacaatcattttaa
aaaagtcttaatctgttatacatctggcagaattgcactatgcttcagtttacgccctctctgtgcctccgcaaaaacatttaaacagacatgggaagat
gtgagatagtggccaggtgaagcagaaaaggaaagagcaggtaacaagaaagcatctatatctctgtttactctaatgtataacgttaatcgtatgttct
tatgaaaaataaaaagagttgttaaaagaaaaaaaaaaaaaaaaa
5' EST: EL710876 [+]ggagaaggaaagcagatccctgcgcactgacagccccccgtgtattcgccaagagccatggagacccccgggcagaagcgggcgacccgcagcacccaca
ccccactctcccccacccgcatcacccgcctgcaggagaaggaagacctgcaggaactcaacgaccgattggccgtctacatcgacaaggtgcgctcctt
ggagtcggagaacgccgggctgcgcctgcgcatcactgagtccgaagaggtggtcagtcgcgaggtgtcgggcatcaagggggcctacgaggcggaactg
gcggacgcccgcaaaactctcgactcggtggccaaggagagggctcgcctgcagctggagctcagcaagatccgcgaggagcacaaggagctgaaagcga
ggaacgccaagaaggagagcgatctgctgacggcgcaggcgcggctgaaggatttggaggccctgctgaactctaaagatgccgccctcaccacggcgct
gggagagaagaggaatctggagaacgagatcagggaacttaaggctcacattgcaaagttggaggccagcctcgccgacacaaagaaacaactgcaggac
gagatgctccgccgcgtggataccgagaaccgcaaccagacgctgaaggaggagcttgagttccagaagaacatttctaacgaggagatgcgcgagacca
agcgccgccacgagacccggatggtggagatggacaacgggcggcagagggagtttgagtccaaactggcggacgccctgcaggatctccgtgcccaaca
tgaggggcaggtcgg
3' EST: EL710875 [+]ctttataaaattctcgcagtataaaataaaaacaaaattttcataaaaaaaccaaaatctctctgtttcgctcgggcacttgggcacttgggcacttggt
gacggcggcgtcgggcagcggattacattatgctgcagctctgcggggtgacctgggcacgctggctgtcgttcccgacgatgtaggtcctggtgaccag
actctccccgagggcactgcccccgctggttcccccggtgctgcggtaggtccgggtcagggtgacgctggaggatgaggaaccggtcgaggccgtggcc
aaaccagaaccctgggagcctagaacccccttctcagccgggcgcccacagctggtgcaaacgatggtgcgagagcgcaggttgtactccccggggtccc
cgctattctgcccatcatgatgatgatgatgatggtggtggtggtgatgtcccacatcgtcatcatcttcatcatctccatcttcatcattgatgacgac
agtgcgcaccagtttcctcatagcgacctcctcattgttagacgtgagcagcgcggtgcgaatactgtcgcctgttccccaggagctctgggccttccat
actaaatcagtgggggggttatttgtggctccggcgcctgatgcccagatcgtcaccgtttgtccggcctttagtgtaagtctgggggggaacctgtaca
caatgggggtctcctcccctatctggcgcttgatctgccagttccccagcgattggtcctcgttactcttgttccgcagccgcacgt
Library:
Description: The library was made from dT primed cDNA and cloned into vector pCS107. PolyA RNA were primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3') and digested with NotI. cDNA was size selected using 1.1% agarose gel electrophoresis (>0.6kb) then ligated into NotI and SalI digested pCS107 vector. Primary library, non-amplified. Library constructed at the DOE Joint Genome Institute (Walnut Creek, CA) as part of the Xenopus Gene Collection project.Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: