XB-CLONE-2621305
Sequence:
5' EST: EL716247 [+]
cccacaagcagcatctttattcccagattttatatctgcccagaatttacagtgggttctactgtactacaagttattgattctgtatccgaggggaatg
gagagaacatacaaactgaaatgaatgaaatgtgaataatatgttgcggcaaagggtcaaatccaagagcccaagtaatcttgtatgtacggtttagcag
ccattcatggaacctgctgtaatatcccttgtgtaacccagacactttcattccttatgtgactttgcatacacaaactgtgggttcctatgggcagatt
gtgctgactcagtccccagattatgtctctgtgtcaccgggagaaactgtcaccttcacatgtaaagccagtagcgatgttagcggcatcctacagaaat
cagggcaggcaccaaagctgctgatctattatacaagcaaccgacacacagggaccccagagcggatcagcggcagcggttctggaactgatttcaccct
tacaatcagcagaatggaagcagaagatgcagcagattattactgccagcagggttacagctggccgttcacttttggtggaggaaccagggtagagatt
aaacgagcagacgccattccggtagtattcatcttcaaaccttcagaggaacaggttaaagaaggaaacccaacagctgtctgtctgatcaacaacttct
atccacggtcggtgacaataaaatggaaggttgacggccag
3' EST: EL716246 [+]gtttgaagcaaaataatttttatttatttttcaacatttcaagacaaaagcagaaacaaataaagcccaaaggctttaggactattgtaaaaacagtgac
attcatgagtaacacaaccaaaaccttgtcatttgtgtgtatctattgagttcttatggcttgtattgggttatgctgcgagacactgtggcacagagag
agtaagattgcaagaaaagacaaaaagacagattataatagagttatatcaggctccctaaggcctgttattattatgtgcactgtgacttaataaaact
ctgtgccagttgttgcgttttgtgttgtactaaacactcgaacttctccgacttatcccacttatccttggtgagcgtcaacatgctgctctggctgtag
gtgttgtcgctttcctgcatatagtcgctgttcttaatgtcggaggcaatgacatcctggccgtcaaccttccattttattgtcaccgaccgtggataga
agttgttgatcagacagacagctgttgggtttccttctttaacctgttcctctgaaggtttgaagatgaatactaccggaatggcgtctgctcgtttaat
ctctaccctggttcctccaccaaaagtgaacggccagctgtaaccctgctggcagtaataatctgctgcatcttctgcttccattctgctgattgtaagg
gtgaaatcagttccagaaccgctgccgctgatccgctctggg
Library:
Description: The library was made from dT primed cDNA and cloned into vector pCS107. PolyA RNA were primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3') and digested with NotI. cDNA was size selected using 1.1% agarose gel electrophoresis (>0.6kb) then ligated into NotI and SalI digested pCS107 vector. Primary library, non-amplified. Library constructed at the DOE Joint Genome Institute (Walnut Creek, CA) as part of the Xenopus Gene Collection project.Anatomy: spleen | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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