XB-CLONE-2610735
Clone Name: IMAGE:8897475
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Gene:
schip1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC153708 [+]
GAGGGGCTCCCGCTGCTTGTGTAGCTAGGTGGGGCGGCTGCTTTGCGTCACACAGCGGCTGAGGGATGTTAGTGCGCTGGGCGCAGGTTCCCCATAGCAG
GATTACAGAGAGGATGGCATGGATTTAGGAAGTGATGCCTGCAGTCGCTCAAGTTCTGAATCCAGCTCCAACAAAGTGACCCCTTGCTCTGACTGTAAGT
CCTCACCCAGTTTGGAACTGGCTGCTCTAGAGGATTATGAGGAGGAGGACTTTCTTGTGGACAAAGTGAGCGATGAGTGGGAAGATGAAGATGAGGAAGA
TGAGGTTGGTGTTGTGGATGGCTTTCCTAAGGACAACTGCTTTGCAAGGATAGGAACTGGAAGAAGTTTGGCAGAAGAGTTTGAGGATGTTAAAAGTGGG
ACTGTTTCTGCCTCTAATGGAAACCCTTCACCTAAGCACAAACTAAATGGAAATTTAGTTGTTGGGAGACCTCCAAGTAAAGGAAAGCACAGCCCCCCTA
AACCACAGCTTAACAATTGCAGGGGAACTTTTGTGAATTCTTTTGGGGGTTCAGGCACACTCATGAGGGAGGAACCACAACTCCCCACAATGGACTGGGA
GGCACTGGAAAAGCATCTGGCTGGCTTACAGTTCCAGGAGCAGGAAGCAAGGAACCAGAGGCAAACAAAGTCGAACTACACATCAGCTCAGAAGAATGAA
AGGGAATCGATCAGGCAGAAGCTTGCACTTGGGAGTTTCTTTGATGATGGCCCTGGCATATACACCAGTTGTAGCAAGAGCGGCAAACCAAGCTTATCTT
CCAGACTACAAAGTGGGATGAACCTGCAAATCTGTTTTGTAAATGACAGTGGCAGTGACAAGGACAGTGATGCTGATGACAGTAAAACTGAAACAAGTCT
GGACACTCCTTTGTCTCCAATGAGCAAACAGAGTTCTTCCTATTCAGACAGAGATACTACTGAGGAAGAGTCGGAGTCCTTTGAAGACATGGATTTTATC
TCTAGGCAAAAGAAACTACAAGCAGAAGCTAAAATGGCACTGGCAATGGTTAAACCAATGGCCAAAATGCAGGTGGAAGTGGAGAAGCAGAACAGGAAAA
AGTCTCCTGTAGCAGACCTTCTGCCACATATGCCTCACATTAGTGAATGCTTGATGAAGAGAAGTTTGAAACCTACTGATCTGAGAGACATGACTATTGG
GCAGCTACAAGTGATAGTCAATGACCTCCACTCTCAAATAGAAAGCTTGAATGAAGAACTAGTCCAGCTTCTCCTAATCCGCGATGAATTGCACATGGAA
CAAGATGCAATGCTTGTAGACATTGAAGACTTGACTAGACATGCTGAAAGTCAGCAGAAGCACATGGCAGAGAAGATGCCTACAAAATAAAGCACAAAGC
CATTGAACTTAGCGGGAAAAAAAGCTAAACTTTACTTTGCTTTTGTGGTTGTCAAAACGCCAATGCTAAAGATGATTCACAAGGAAAATCAGTGTTAGTC
TTTGATAATGTCTGAAGCTTTACGTCCAGTGACTCGGCCTGTGCTTCCTAATTTATTTTAACTTTTTACTCGTGACACTAAATATTAGGCATTTTATTAA
GATACTATGACTATATGTACAGTACTTATGCCATTACAGATTATACAGTAAGAGAGAGAGAGAGAGAATAGTTACAGATTTTTATTTTTTTGGTTTTGGA
GATTTACGTTGATCAGTATTTTTCTTTTTGCCACTGTAGTTTCCAAACACAAATGTTATTGACGGTGCTAAAGAAGTCGACAACTACATGCCTGCCTCAT
TGTAACGTCGTAGCGCTCGGTATATTGTAAAAGTCAATCAGTTCTCAACATTTTGTAAATGTTGATAATTGACTTCCATTTTTAATGTGCTATTATTCAA
TTGGATTGGAGAAAAAAAGTCACTTTCAAAGTTTTATGTATAAATATGTAAAATAAACTTTAAACATTTGTTTCGCAAAAAAAAAAAAAAA
5' EST: EL723163 [+]gaggggctcccgctgcttgtgtagctaggtggggcggctgctttgcgtcacacagcggctgagggatgttagtgcgctgggcgcaggttccccatagcag
gattacagagaggatggcatggatttaggaagtgatgcctgcagtcgctcaagttctgaatccagctccaacaaagtgaccccttgctctgactgtaagt
cctcacccagtttggaactggctgctctagaggattatgaggaggaggactttcttgtggacaaagtgagcgatgagtgggaagatgaagatgaggaaga
tgaggttggtgttgtggatggctttcctaaggacaactgctttgcaaggataggaactggaagaagtttggcagaagagtttgaggatgttaaaagtggg
actgtttctgcctctaatggaaacccttcacctaagcacaaactaaatggaaatttagttgttgggagacctccaagtaaaggaaagcacagccccccta
aaccacagcttaacaattgcaggggaacttttgtgaattcttttgggggttcaggcacactcatgagggaggaaccacaactccccacaatggactggga
ggcactggaaaagcatctggctggcttacagttccaggagcaggaagcaaggaaccagaggcaaacaaagtcgaactacacatcagctcagaagaatgaa
agggaatcgatcaggcagaagcttgcacttgggagtttctttgatgatggccctggcatataca
3' EST: EL723162 [+]gcgaaacaaatgtttaaagtttattttacatatttatacataaaactttgaaagtgactttttttctccaatccaattgaataatagcacattaaaaatg
gaagtcaattatcaacatttacaaaatgttgagaactgattgacttttacaatataccgagcgctacgacgttacaatgaggcaggcatgtagttgtcga
cttctttagcaccgtcaataacatttgtgtttggaaactacagtggcaaaaagaaaaatactgatcaacgtaaatctccaaaaccaaaaaaataaaaatc
tgtaactattctctctctctctctcttactgtataatctgtaatggcataagtactgtacatatagtcatagtatcttaataaaatgcctaatatttagt
gtcacgagtaaaaagttaaaataaattaggaagcacaggccgagtcactggacgtaaagcttcagacattatcaaagactaacactgattttccttgtga
atcatctttagcattggcgttttgacaaccacaaaagcaaagtaaagtttagctttttttcccgctaagttcaatggctttgtgctttattttgtaggca
tcttctctgccatgtgcttctgctgactttcagcatgtctagtcaagtcttcaatgtctacaagcattgcatcttgttccatgtgcaattcatcgcgg
Library:
Description: The library was made from dT primed cDNA and cloned into vector pCS107. PolyA RNA were primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3') and digested with NotI. cDNA was size selected using 1.1% agarose gel electrophoresis (>0.6kb) then ligated into NotI and SalI digested pCS107 vector. Primary library, non-amplified. Library constructed at the DOE Joint Genome Institute (Walnut Creek, CA) as part of the Xenopus Gene Collection project.Anatomy: spleen | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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