XB-CLONE-2610082
Clone Name: IMAGE:8897722
|
Gene:
mhc1-uaa
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC154904 [+]
GTATACTATCAGAATGGATGTGCGTCTAGTTCCCCTCCTGCTCACTTTGGGGGTATCTGCAGTGTATTCTGGCAGTCATTCCCTGCGCTACTATTATACA
GCAGTCTCAGATCGAGCCTTTGGGCTCCCAGAATTTTCCACAGCTGGGTATGTGGATGAGACACAGATTGAAAGATACAGCAGTGACAATGGAAAGTCTG
AACCTGCAGCTCAGTGGATGAAGCAGAATGAGGGTCCTGAATACAGGGACGAACAAACACAGATCCTCAAAGGCAACGAGGCTGTGTTTAAGCACAATGT
AAAGGTTGCAATGGATCGCTTCAACCAGACCTCAGGTACCCACATCGTTCAGAGCATGTACGGCTGTGAGCTGCGAGAGGACGGCAGTACCCGGGGGTAT
GAGCAGGATGGATACGATGGGAGAGAGTTCCTAGCGCTGGATACAGAGAGATGGGTGTATGTACCTTCCATGCGCGAGGCGCAGCTCACTACCCAGAAGT
GGAACAGCCCGGAGGTTAATGAGCCTGAGAGAGAGAGGAATTACCTGGAGAATATCTGTATCGAGTGGCTGAAGAGATACCTGGGCTACGGGCAGGCAGA
GCTGGAGCGGAGAGTTCGCCCTCATGTAAAGATTTCAGATCGCCAATCAGATGGCAATACAGAGCTTCACTGCCAGGCGTATGGATTCTATCCCAGAGAA
ATAGATGTCAAATGGGTGAGGAATGGTAAAGATGATGTACACTCGGAGGAAGCCAAAGAGATCCTTCCCAACCCTGATGGCAGCTACCAGCTCAGAGTCA
CTGCTGAGGTTACACCCAAGGAAGGTGACAGTTATGCCTGCCATGTGGAACACAGCAGCCTGGAGGAGAAGCTCATTGTTGTGTGGCCAGGCCCCAATAA
AGATGGAAAAAACATGGTTATTATTATTGCTGGTGTTGTTGTTGCTGTACTGCTGATCACTGCTGCTGTTGTTGGATTTGCTTTCTACAAGAAAAGGAAA
GCCAAGTCTGGTTACACAGAAACTGCAAGTAATGATTCTCCTGCTTCATCTGTTGCTAGAGCTTAAGGGTGACGTGTTCTAAGAAAGAAGCAGTGAAATA
TGCGGAAGTAGAGGAGACAAGAAACCTGATTTGATTTGTTTGCTTCTGTTCTAAAATATATTTCCATTATAGAAAGGAATGTTTTTAATTTGCTTCATCA
TAAATATTGGGTTGTTATAACATTCAATATTCAGCATTCTGGTGTGATCATATTGCTTAAATATAAGGGTGTAGGTTTATGTTGTTTAAGTTTTAAATGT
TTTTGATTTTTGAATTGTAAAAATGAATGTAAAACATGTGCAACTTATCCATGTAGCACAGTGTATGGTAGAATTATTTGATTACATTTGCAGGGATTGT
ATAAAGCTAAGAACAAACTGGTTCGTCAGTTGTATAATTTCCTTTTCTACCCAGAATACCAAACTGGCATCTGAGTTTCATATTTCTTCTCATAAACTCC
CACGGAATGTCACCTACTGTATCTTGTATTGGCAGTGTTGTCATATTTGCCATTATTATTATTATTATTATTATTATTTTACAAGTGATTTCATGTAACC
TTCTGCTCAATTGGCTCAATTATCTGTCCAACCAGTGCATACAGGAATTATGCAAGCACCTTACCTTATAGCATTCATGGCCTCCCAGTACTTGGCCTCC
TCTCTCCTGCATGCCCTCAGCTTTCAGTGCTCATGTTTTTTCTACACCCTAAAGTCTAACAAGCCCTCTTCAGGAGTATGTCATACACAGCTCTCAGCAC
CATTTTTCAAATTTAGAAATTCCTGAACAAACCCCTTCCTTACTGCTTCTGTTTCCAGGCATGACAAGTTCCAGGATTGTAGAACTGGAAACTTTGGTTC
TCTGCGTCTCTATACAGTTGTCAGTGGATTGGAGGGAACTGTTGTTCAGTAACTGGTCCAGGATAAAAGTAAATGTTCCCTACTATGGCTGACATTATGA
AAGAATGAAAACCCTTGAGAAAATTTATAGCTGGGGCTGATGCCACCTCTTATTCAACTTACAATTCAAAAGCTGGGGGGTTGAAGCCTGGGTGCTCACA
TGTGGTACAGAGGTCACAGTGTGGGTCATACAGTGGTGTTGTTCTGGATATGAACCAGGGCAGAACTAAGGGTAGGCAGAAGAAGCATGTGCCTAGGGTG
GGGGCTGTCTTGCAGACCCTGCAAGAGTGTAGAGGGCTTAGTAGGGCTCTAAGTAATGAGTGACTTCAATAGACCTTAGTAGAATAGTTAACTCACCAAT
GTCTGGGGCCCTACACTGAATTTGCTGTGGGGCCCAGTAATATGTAGTTATGGCACTGGAAATGCCCATCATGATGGGAGACACCATGTCACTTTCAGTG
GAACCACATATAGCGGTCATAACACAACAGTGATTGGTCTAATGTTGTCTAAGGAAAGTATCCTCATAAAGAAAGCAATATCAAAGTTCCTCTTATGCAG
AAAATTTATGTTTTAGGAATCAAAGAAAATTTCCAATTAACATCAGAGTACATTTTTCTTTGAATAGAAACCTTAACCAAGAGCCTTTCTTGTGAACAGG
AGTAGAAGCAACAGTTTCATTGAATTTAAAAATTTGCCTGCTGTAAGTTTTTCTCTGAATAAATTCTAAATAAAATCTCTATAAATACACAAAAAAAAAA
AAAAAA
5' EST: EL723592 [+]gtatactatcagaatggatgtgcgtctagttcccctcctgctcactttgggggtatctgcagtgtattctggcagtcattccctgcgctactattataca
gcagtctcagatcgagcctttgggctcccagaattttccacagctgggtatgtggatgagacacagattgaaagatacagcagtgacaatggaaagtctg
aacctgcagctcagtggatgaagcagaatgagggtcctgaatacagggacgaacaaacacagatcctcaaaggcaacgaggctgtgtttaagcacaatgt
aaaggttgcaatggatcgcttcaaccagacctcaggtacccacatcgttcagagcatgtacggctgtgagctgcgagaggacggcagtacccgggggtat
gagcaggatggatacgatgggagagagttcctagcgctggatacagagagatgggtgtatgtaccttccatgcgcgaggcgcagctcactacccagaagt
ggaacagcccggaggttaatgagcctgagagagagaggaattacctggagaatatctgtatcgagtggctgaagagatacctgggctacgggcaggcaga
gctggagcggagagttcgccctcatgtaaagatttcagatcgccaatcagatggcaatacagagcttcactgccaggcgtatggattctatcccagagaa
atagatgtcaaatgggtgaggaatggtaaagatgatgtacactcggaggaagccaaagagatccttcccaaccctgatggcagctaccagctcagagtca
ct
3' EST: EL723591 [+]gtgtatttatagagattttatttagaatttattcagagaaaaacttacagcaggcaaatttttaaattcaatgaaactgttgcttctactcctgttcaca
agaaaggctcttggttaaggtttctattcaaagaaaaatgtactctgatgttaattggaaattttctttgattcctaaaacataaattttctgcataaga
ggaactttgatattgctttctttatgaggatactttccttagacaacattagaccaatcactgttgtgttatgaccgctatatgtggttccactgaaagt
gacatggtgtctcccatcatgatgggcatttccagtgccataactacatattactgggccccacagcaaattcagtgtagggccccagacattggtgagt
taactattctactaaggtctattgaagtcactcattacttagagccctactaagccctctacactcttgcagggtctgcaagacagcccccaccctaggc
acatgcttcttctgcctacccttagttctgccctggttcatatccagaacaacaccactgtatgacccacactgtgacctctgtaccacatgtgagcacc
caggcttcaaccccccagcttttgaattgtaagttgaataagaggtggcatcagccccagctataaattttctcaagggttttcattctttcataatgtc
agccatagtagggaacatttacttttatcctggaccagttactgaacaacagttccctccaatccactgacaactgtata
Library:
Description: The library was made from dT primed cDNA and cloned into vector pCS107. PolyA RNA were primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3') and digested with NotI. cDNA was size selected using 1.1% agarose gel electrophoresis (>0.6kb) then ligated into NotI and SalI digested pCS107 vector. Primary library, non-amplified. Library constructed at the DOE Joint Genome Institute (Walnut Creek, CA) as part of the Xenopus Gene Collection project.Anatomy: spleen | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: