XB-CLONE-2585542
Sequence:
5' EST: EL671576 [+]
ccaccctgagcaggaacagaactctggggaacatagcggaacgattcctttctgctcagctggatctgggggaaagtaggatctactgcccttactgtgc
ccgtgatctcctgtgcgatggccacgtgggggtccatagaaagtctgaagaacatcaaccaacagatcccaccactgcccttgggaacagaaaatgttct
gcccataacaaggttctagagtatcagtgctgtgaggatggggcccctgtctgtgtgtcctgctgcctggccggggggcaccggggccacagggtggagc
ttatgagtgaggcctctgagaagaagaaggagaaactgaggaaagtctggaagccctagagaagcgactcctgagtgacatctccaggcagaaagaggag
ctttttatccaactccatgacctgatccaacagctggagataaagaaggacgagctgtctgggaagatccatcacattgaggagctgtgcaacatggtgg
atccactcactgtcctactggaaccagttggggctgagggggcagatactgagggcagagggagggagggtataaaggtcccggctgtaggggatctgga
tgtggttctgatctcagagacattactcacagggttggatcagattgtgactggggtaaaggggaggatctactgggaggaggctatggacctggtactg
gatataaacacggccgggaattatctgtccgtaacattggatgaaaaatgcgcctccccctcactaacggaccanggtcgcccccaaaccccacgcaggt
3' EST: EL671575 [+]ctttggaatctcaacatttatttttatacatttataacatgcaatgaattgtataaacatggcggaagctgtagtctggggttactggtggccttcacca
ttatattgtgtatcagcctatggtctatgtgtgtttgtggggggggggtcattagctccatcgttagctgtaatcgtaattagttacctaaactggagac
agaagtgaagaaaattccagaacaatggtcaccgacacctacagtttatggtcaacattgatgggtttccattctaggtaagtggcctaactaatgatcc
tcccccattctaggtaagtggcctaactaatgatcctcccccattctaggtaagtggcctaactaatgatcctcacccagggattatccatgcataccca
gaatgcagcatgaaggggctgaacaaaggaggcggtgaaggtgtgtaagtgcctgattggctcgctcagctcatagaaggacagacgtccggcctcgtag
tccaatgagatcctgattctcctgcaggaagggacgtggggtaaatacgtgtccttccagtcgtgtctcaccgagtaggtcttgcaccctcggcacaaac
accaggacttgtcgttattcccaatccaggaattctccccgcccctctctatactgggataggccacgcccaccctccaactcccggactcactgacctc
cacctcccagtaatgtcgccccgaggggaagctcctggtgctcaaagcctgagggacctggaacctgcgtggggtttgg
Library:
Description: The library was made from dT primed cDNA and cloned into vector pCS107. PolyA RNA were primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3') and digested with NotI. cDNA was size selected using 1.1% agarose gel electrophoresis (>0.6kb) then ligated into NotI and SalI digested pCS107 vector. Primary library, non-amplified. Library constructed at the DOE Joint Genome Institute (Walnut Creek, CA) as part of the Xenopus Gene Collection project.Anatomy: thymus | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: