XB-CLONE-2580118
Clone Name: IMAGE:8927510
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Gene:
vsig1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC154930 [+]
AGTGATTCAAACCACCTCAAGAAAACATCTGCTCCCTGTTCTCTCTGGATCTCTAGGCACCATGAGTTTCCTCCTGTTTGTAGCTTTAGGACTGAGTCTT
ACAGCCTTGAGCCACTGCGTCCAGGTGACTGTACGGAGCCCATATGTCAACGTGACCTCAGGACAGAATGCGACCTTATACTGCACCTACGCTCTCAACA
ATCCACAAAGCACCAAAAATCTTGTAATTCAGTGGAACGTTTTTCAGGCAAAATCACAGAATCAGGAAACAGTGTTCTTTTATCAGAATGGCCAGCCTTT
CTCTGGGCCCGGTTATAAGAACAGAGTCACTGCTGCCCTAACTCCAGGGAATGCAACCATAACTATTTCCAACATGCAGCCAGAGGATACCGGTGTCTAC
ACCTGTGAAATACTGAATTTACCTGAGTCCAGTGGCGAAGGAAAAATTATCGTGACTGTTCTGGTTCCACCATCTAATCCACATTGTACTATCCGGGGAG
CGGTAGAGACTGGACATTTCATTTCTCTGATGTGTTATTCTGAGGAGGGGATGCCGCGCCCGCTGTATACTTGGAATAGAGTGGAGAATGGGGTATTGAA
GTCAACACCTCCTCAGATGAATCAGCAGAAAGGAAGCCTCATAATAGGCAACCTGACAGATTTTGAAGAAGGATACTACAGATGTACAGCCAGCAACGCA
CTTGGAAATGCTACATGTGAACTCAATTTACACACAGGGGGAGAAGCAGGTGTAATTGCAGCAGCAGTCATCGGAGGACTGTTGGCAGCAGCTATCATTA
TCGCCATCGTCTGGTTTCTTATAGTAAAAAGAAAGCAAAAAAAGCAAATTCCACCAACAAAGGAAATGAAAACAAGTCCTTCAGGAGAAACCCATGAGTA
CACGGCAGTATCTGGAGAAGCTAACGAGCCCAACAAGGAAACTCTGGGGTCTTCTGAGCCTACCGAAACCATCCAGTTCCATGACCGTGCTGAGAATGCA
GCCAATGGAGAGACAGAAGAACACGCTGCATAAAGCAAAAGGCTCGAATAATTTGCTTATATAAAGGCACTGTTAAGGCTACACATTTTTTTTCAAATCT
GCGTTAAACAATATAGTCACCTTATATGTGTACATACAGTATTCTGACTCACACAAATGTTTGCAGAGTTAAGTACAAATCTGGCAATAGGTAGAACAGG
AGAGAAGACATGGCAGTAAATCATTCTGTGCCTGGAAAGCAAGGAATTTTAAGCCAAAGAGCCAGACTATATTAAATTCTCACAATAGCACTTGCAACAG
ATTACAAAAGAATGTTGTATAGCCTTTACATTCCCTTTAACATCAGATTAAGATGGGTTCCTGCTCACACAAACTGAAAGAGCAGAAATCCATTTTTTTT
ACTAAAAAAATGATAAATATTTACATTGAGAAGCAAGAGAAATTCTTTTTATACTTATGTTTTGTTGCAATTATTTGCTGTCACTGTATACAAAATAATA
GCCTTCGTCATTTGTTAATGCTATATATCATGCTATTCCCTATGTGTAATATCTGGGTCTCTTATTCACTGCCTGACACAGTAGTTTCATAAATTACATT
TTAAGGACAAAAGGGGGATGGCAGAGAAGGGGAAACTAAAACTAAATGACAAAATAGCAGAGCTGGGTCGAGACACCTGAGAACCCAAGAAAAGGGGGAT
TTCTACATGCAACCCCACTAGGGTCCCACCAAACCTTTACCTCTATCTGGAGCAAAAAGTTATTGTTGGACACAGGAAGGGTGAGGTGATAAAAGGAGCT
GGACTAATGATAAGTCTTTCATATAGTATGTGGCTGTGCATGCCTCAATAAACAAATATTTTTTTTTATTTTAAGGAGACCACGCACTAGCTGAGCCTCC
ATGATATCTGGCTAATCACACCCACGGCCAAATGGACAGATACCATATGACTGACCATGCACATGTATAGCCACATTCTATTGTTCAATCTATTTGGGCC
AACAGCATCCGACCTACAGAAATCTGGGCATGAATGGATAACTTAATTAGAAATACTGGTTTTTACAGTAGCATGTTAGTTAAAAACTCTGCTAAATACA
ACCATCTTTTTTATTGTCTAAATAAATCCTTACCCAGTCTATTTTATTTTAATAGAAGCAGCTGGGCAGTTTGTCCAATTGTGACCATAATGTCTGACTG
TCTGGGTGTACATGTCAGCAATGGTTTCAGTTCTGATAGACTAACCCACAATCAGATCACAGTCAGTCCATCAGACAATACACCTTCTCAGTGTGTGCAC
AGTACAATGGTACATGAGCAAGCGAAAGAAGTTGCCCTCCTTCGCATCCCTGTAGATGGCCAAATCACAGTATCTGTTAGTCTCTATCAGTCACATACCT
GCCAAGTTTTCATAGCTGCAGACATTATAAGTGCAATGGCACATACAGCAATCTGTAACTCATGGGAAATTGCTGTTCCCACAGTTGACCATTCTCCAGC
GGGCACCAAATTGCCGTGTGTGCTGTCACCATATGGCTCATTTACAAAAGGATGCAAGGTACAAAAAGTCTAAAAGCCCCACACCTACTGCCTGGCTGAG
TATAAGAGTTACCTTTCACAGAAAGTGTTGTGCTCTGCATGTTGTTGGTATAAGAATGTGTGTGTAGCTATTGCCTCTGACATTAATAAATTACAACCAA
TTTGTAAATAAAAAAAAAAAAAAA
5' EST: EL675090 [+]tgattcaaaccacctcaagaaaacatctgctccctgttctctctggatctctaggcaccatgagtttcctcctgtttgtagctttaggactgagtcttac
agccttgagccactgcgtccaggtgactgtacggagcccatatgtcaacgtgacctcaggacagaatgcgaccttatactgcacctacgctctcaacaat
ccacaaagcaccaaaaatcttgtaattcagtggaacgtttttcaggcaaaatcacagaatcaggaaacagtgttcttttatcagaatggccagcctttct
ctgggcccggttataagaacagagtcactgctgccctaactccagggaatgcaaccataactatttccaacatgcagccagaggataccggtgtctacac
ctgtgaaatactgaatttacctgagtccagtggcgaaggaaaaattatcgtgactgttctggttccaccatctaatccacattgtactatccggggagcg
gtagagactggacatttcatttctctgatgtgttattctgaggaggggatgccgcgcccgctgtatacttggaatagagtggagaatggggtattgaagt
caacacctcctcagatgaatcagcagaaaggaagcctcataataggcaacctgacagattttgaagaaggatactacagatgtacagccagcaacgcact
tggaaatgctacatgtgaactcaatttacacacagggggagaagcaggtgtaattgcagcagcagtcatcggaggactgttggcagcagc
3' EST: EL675089 [+]atttacaaattggttgtaatttattaatgtcagaggcaatagctacacacacattcttataccaacaacatgcagagcacaacactttctgtgaaaggta
actcttatactcagccaggcagtaggtgtggggcttttagactttttgtaccttgcatccttttgtaaatgagccatatggtgacagcacacacggcaat
ttggtgcccgctggagaatggtcaactgtgggaacagcaatttcccatgagttacagattgctgtatgtgccattgcacttataatgtctgcagctatga
aaacttggcaggtatgtgactgatagagactaacagatactgtgatttggccatctacagggatgcgaaggagggcaacttctttcgcttgctcatgtac
cattgtactgtgcacacactgagaaggtgtattgtctgatggactgactgtgatctgattgtgggttagtctatcagaactgaaaccattgctgacatgt
acacccagacagtcagacattatggtcacaattggacaaactgcccagctgcttctattaaaataaaatagactgggtaaggatttatttagacaataaa
aaagatggttgtatttagcagagtttttaactaacatgctactgtaaaaaccagtatttctaattaagttatccattcatgcccagatttctgtaggtcg
gatgctgttggcccaaatagattgaacaatagaatgtggctatacatgtgcatggtcagtcatatggtatctgtcc
Library:
Description: The library was made from dT primed cDNA and cloned into vector pCS107. PolyA RNA were primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3') and digested with NotI. cDNA was size selected using 1.1% agarose gel electrophoresis (>0.6kb) then ligated into NotI and SalI digested pCS107 vector. Primary library, non-amplified. Library constructed at the DOE Joint Genome Institute (Walnut Creek, CA) as part of the Xenopus Gene Collection project.Anatomy: thymus | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: