XB-CLONE-2577232
Clone Name: IMAGE:8928537
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Gene:
commd8
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC153686 [+]
GCTATGGAAGGAGAAGCGCATAGCGGGTTGCGTCTGATCGGGAAGCTTCCCGCAGCGCAGTGCCCACAGTTCTTTCACAAAGTCATAGATGGAGTATGTG
GCCGGGCACAGCCTCGTTTCCAGGATTATGGCAATATATGGAGCCTACAGGAATGGCTGGAAGTTCTAGAAGACTCCACAGCTTTCTTCAAGAACAGTAT
TGGTGCTGATGTAAATGCAGATGAGGTTTCTAATGGGTTAAATGTGCTCAGTGCAGAACACCAAGAAGTGATCCTAAAGTGTTTGAAAAGTAGAAAACCT
GACATAGCAGAAGCTGTTGTGGAGAAGGCGTGTGCTATGTCATCTGCATATTTACAGGATTTTGACTGGCAGTTAAAGCTTGCCCTTTCTAGTGATAAGC
TCTCAGCACTGCAGATGCCACTTGTTAATCTTGATTTGGATATTGCTAAAAATGGGTCGGTTACACCTGTCTCTATAGAGATGAACAAAGAGGAGCTTCA
GAACCTTATAAATTCTCTAGAGGCAGCTAATAAGGTCGTCCTTCAGCTCAAATAACAGGAAACTGCTTCTACCTAATGTTGAAGAAGTGACCAGAATGTA
AAAAGAGAAAGGACAATACTCTTTTGATGGAATTTTAATCATTTTTAAAAGGAGCCACTACATGAAGGAGCATATTCATCTCTTGGCAGCCCTGTACTTA
GTGCTAGAACCGTTCAGGACCTTTTTTTTTTTTTTTCTTGTTCCCGAATGTTGCCATTAACTGCGTGGGCGATAAATGTTCTACCAATATGAATTCTGTC
CAGTGTTTGATATCTGCCAGTAGCTTATTGAGTTTAAAGGAATACGATATATTGAATACCATAAAATAAAGCGTACCTGTATTTTTCATATGCTTTTGCG
TTCCAGAAACATTTCTTTATGCCGAATCCCTTGTTCCGAGGCTTAGTGATTGCAAACCTATATTTTGTGTGCCATTTTGGAAATGACTATGAAATAACGA
TGAAGTCATCTGCGCCAGAGATTTCTCTTTATACTTTCTCAAATGAGCCGTTTGCTTCTATACAGCTGTACAGGACTAAGAGAAAGCTTTCCAAGACTCT
TCACAAAATAATTGATATACACAGTAGATTTTTAGTACCACATGCTGGTTGGTATTTTGTGAATCTATGCTTGTGGAATGATAATGCGATTGTCTGTTTA
CCCAGAGCTGAAAGCTTAAATATTCCCATACTGTGTACTTTAATGAAAAGAAATACAATAAATATTGTAATTTCTTGCACCAAAAAAAAAAAAAAA
5' EST: EL676959 [+]ggctatggaaggagaagcgcatagcgggttgcgtctgatcgggaagcttcccgcagcgcagtgcccacagttctttcacaaagtcatagatggagtatgt
ggccgggcacagcctcgtttccaggattatggcaatatatggagcctacaggaatggctggaagttctagaagactccacagctttcttcaagaacagta
ttggtgctgatgtaaatgcagatgaggtttctaatgggttaaatgtgctcagtgcagaacaccaagaagtgatcctaaagtgtttgaaaagtagaaaacc
tgacatagcagaagctgttgtggagaaggcgtgtgctatgtcatctgcatatttacaggattttgactggcagttaaagcttgccctttctagtgataag
ctctcagcactgcagatgccacttgttaatcttgatttggatattgctaaaaatgggtcggttacacctgtctctatagagatgaacaaagaggagcttc
agaaccttataaattctctagaggcagctaataaggtcgtccttcagctcanataacaggaaactgcttctacctaatgttgaagaagtgaccagaatgt
aanaagagaaaggggacatactctttgatggaattttatca
3' EST: EL676958 [+]ggtgcaagaaattacaatatttattgtatttcttttcattaaagtacacagtatgggaatatttaagctttcagctctgggtaaacagacaatcgcatta
tcattccacaagcatagattcacaaaataccaaccagcatgtggtactaaaaatctactgtgtatatcaattattttgtgaagagtcttggaaagctttc
tcttagtcctgtacagctgtatagaagcaaacggctcatttgagaaagtataaagagaaatctctggcgcagatgacttcatcgttatttcatagtcatt
tccaaaatggcacacaaaatataggtttgcaatcactaagcctcggaacaagggattcggcataaagaaatgtttctggaacgcaaaagcatatgaaaaa
tacaggtacgctttattttatggtattcaatatatcgtattcctttaaactcaataagctactggcagatatcaaacactggacagaattcatattggta
gaacatttatcgcccacgcagttaatggcaacattcgggaacaagaaaaaaaaaaaaaaaggtcctgaacggttctagcactaagtacagggctgccaag
agatgaatatgctccttcatgtagtggctccttttaaaaatgattaaaattccatcaaaagagtattgtcctttctctttttacattctggtcacttctt
caacattaggtagaagcagtttccctgtatttgagctgaaggacg
Library:
Description: The library was made from dT primed cDNA and cloned into vector pCS107. PolyA RNA were primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3') and digested with NotI. cDNA was size selected using 1.1% agarose gel electrophoresis (>0.6kb) then ligated into NotI and SalI digested pCS107 vector. Primary library, non-amplified. Library constructed at the DOE Joint Genome Institute (Walnut Creek, CA) as part of the Xenopus Gene Collection project.Anatomy: thymus | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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