XB-CLONE-2574854
Clone Name: IMAGE:8929520
|
Gene:
XB5952892
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC157673 [+]
GGACTGGAAGCCAGTAGCAAAGTCTCATCATTTCAAAGCTGTATACTTTTGTTAGCAGACAAAGGTTAAGAGATGCCGTTTCTCCATACATTTGCTCTTT
TATGTGGTGTATCAGTTATAGCAATCAGTGCCCAGATGAGCAAGGAGGCTGAAAAAAATGCATGTGCACTCATTACATGTGAATCCACGGGAAAAGCAGG
ACCACCAGGCAAAAATGGATTGCCAGGAGCTAAAGGAGAGAAAGGAGAGCAAGGACTACCAGGACTAAGAGGTCCAGAAGGGCTTCGAGGAATCCCTGGG
CCAATGGGACCCAAAGGAGAGCAGGGTCCACCAGGTCCAAAGGGTGACCGAGGAGAAAGTTCTGCTTCAGAAGTGGAAGCCCTTAAACTCCAAACATCCT
TGTTAGTTGGACGGCTGAATGCACTGCAATCTTCACTCGAGACTCCAAAGAAAGCTTTTCTCTTTGCCAGCGGAATAAGTATTGGGAGCAAATACTATGT
GACAAATAGACAGGAAGGAAATTATTTTGAAGGAAAATCTATGTGCCAAAGAGCTGGTGGTCAGCTGCCTTCTCCAAAGAATGCTGCCGAGAATGAAGCT
CTCACTGTTTTAGCTGCACGCTACAAGAAGCAATTCTTCTTAGGTTTAAATGATTTACAAACAGAAGGCACGTTTCTGTACCCAGATAGCTCAGCGGTTG
GCTACGTCAACTGGGATGTGCGAGAACCCAATAATGACAAAGGTGTAGAAGACTGTGTTGAAATTTACACAACTGGAAAATGGAATGACAAAAGCTGTCA
GGAGATCCGTTTGATTGTATGTGAATTTTAATCTTAACTGATGAGCGCCCTGCAAATTATACATCCAATTGATGCTTGTAGCACTTAGTAGTACTCTGCA
CCTTCATGTTAAAATGACATGTATCAAATCTAACATGTATTTCAAGAATACGCATGAACCAAAATTAATAAATAAAGCAGATTAAAATGTAAAAAAAAAA
AAAAA
5' EST: EL678514 [+]ggactggaagccagtagcaaagtctcatcatttcaaagctgtatacttttgttagcagacaaaggttaagagatgccgtttctccatacatttgctcttt
tatgtggtgtatcagttatagcaatcagtgcccagatgagcaaggaggctgaaaaaaatgcatgtgcactcattacatgtgaatccacgggaaaagcagg
accaccaggcaaaaatggattgccaggagctaaaggagagaaaggagagcaaggactaccaggactaagaggtccagaagggcttcgaggaatccctggg
ccaatgggacccaaaggagagcagggtccaccaggtccaaagggtgaccgaggagaaagttctgcttcagaagtggaagcccttaaactccaaacatcct
tgttagttggacggctgaatgcactgcaatcttcactcgagactccaaagaaagcttttctctttgccagcggaataagtattgggagcaaatactatgt
gacaaatagacaggaaggaaattattttgaaggaaaatctatgtgccaaagagctggtggtcagctgccttctccaaagaatgctgccgagaatgaagct
ctcactgttttagctgcacgctacaagaagcaattcttcttaggtttaaatgatttacaaacagaaggcacgtttctgtacccagatagctcagcggttg
gctacgtcaactgngatgtgcga
3' EST: EL678513 [+]acattttaatctgctttatttattaattttggttcatgcgtattcttgaaatacatgttagatttgatacatgtcattttaacatgaaggtgcagagtac
tactaagtgctacaagcatcaattggatgtataatttgcagggcgctcatcagttaagattaaaattcacatacaatcaaacggatctcctgacagcttt
tgtcattccattttccagttgtgtaaatttcaacacagtcttctacacctttgtcattattgggttctcgcacatcccagttgacgtagccaaccgctga
gctatctgggtacagaaacgtgccttctgtttgtaaatcatttaaacctaagaagaattgcttcttgtagcgtgcagctaaaacagtgagagcttcattc
tcggcagcattctttggagaaggcagctgaccaccagctctttggcacatagattttccttcaaaataatttccttcctgtctatttgtcacatagtatt
tgctcccaatacttattccgctggcaaagagaaaagctttctttggagtctcgagtgaagattgcagtgcattcagccgtccaactaacaaggatgtttg
gagtttaagggcttccacttctgaagcagaactttctcctcggtcaccctttggacctggtggaccctgctctcctttgggtcccattggcccagggatt
cctcgaagcccttctgggactcttagtcctggtagtccttgctctcctttctctcctttagc
Library:
Description: The library was made from dT primed cDNA and cloned into vector pCS107. PolyA RNA were primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3') and digested with NotI. cDNA was size selected using 1.1% agarose gel electrophoresis (>0.6kb) then ligated into NotI and SalI digested pCS107 vector. Primary library, non-amplified. Library constructed at the DOE Joint Genome Institute (Walnut Creek, CA) as part of the Xenopus Gene Collection project.Anatomy: thymus | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: