XB-CLONE-2552462
Clone Name: IMAGE:8906120
|
Gene:
mien1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: EL798488 [+]
ggaaaaatgtccgtcagtatcgtggtggagtattgcgagccctgcgggttcaagtctcactatgaagagttggcaagcgccgtgcttgaagaatttccag
acgtcaccattgactcccgccctgggggcacaggtgcctttgagattgagataaacggacagctggttttctccaagctggaactgggggggtttcccta
tgcgaaagatctcattgaggccatccgaaaagccagcaatggggagccagtggagaagatcaccaatagtcaggccccgtgtgttattttataactctcc
aggtgccacctttctaccatgactgctgtaatgaattgaatgacttagcgctgcctttataacagtagcattggttcctttaggtcttgaccattgtgtc
ctcacctgtaagtgaaacctgttggtatgttccagccattaatagccataaggaaacaattatactctaacgctgaaagcaaaaaatgggaacacactgg
aaaagccacaatggtgcttaggattgacagtaactaccatttctcaactgcctgtcccctacatacacatcattccctcttctgtcaaaggtttgtgtca
cagccatacatattgtccatatatccatatgcctaaagcagcttggaattaacccatgtccatagtaggccaggattatgtacttaattatgtaattaat
tgcttaaatgcagatgagctaaagggaattttggctcacttttctatttattggggcatacgtaatccttgtgctttttcca
3' EST: EL798489 [+]tttttttttttttttatatttagaactgaacattttaatgcattatttacacctttttaaataaatacaggtgtcattattgtaaaatgtaataatcctt
tgcctgtcagtatcaggagaggttaccctgaagcctgaagtgctccattatgccaatatgagaagtgagtcaatattagccacacagtattagtgtttat
atctggatgtaatattagacctaacacacataacttggagacaaatactgaattctgggctgctaatagctacccacttccttcattaggccccctgtcc
catacatactaaaccacagtaattggtcttcgagttgtaccccgattgctgccagggctttgggtattgaggcaaacaatgaagccaaattctaatttgt
gctggaagagtctcaaagtagtcagccagatcagcaggagaatagagggccgggcttaggcaactgttccaaaccatattattaatcatgaaaaggctgc
atattttttaattgatattatattgcaaagttgcttgtgtttgggtggacttcccctttaatagtaatctatatctggtgtaaattcccccactctggaa
aaagcacaaggattacgtatgccccaataaatagaaaagtgagccaaaattccctttagctcatctgcatttaagcaattaattacataattaagtacat
aatcctggcctactatggacatgggttaattccaagctgctttaggcatatggatatatggacaatatgtatggctgtgacacaaacctttgac
Library:
Description: The library was made from dT primed cDNA and cloned into vector pCS107. PolyA RNA were primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3') and digested with NotI. cDNA was size selected using 1.1% agarose gel electrophoresis (>0.6kb) then ligated into NotI and SalI digested pCS107 vector. Primary library, non-amplified. Library constructed at the DOE Joint Genome Institute (Walnut Creek, CA) as part of the Xenopus Gene Collection project.Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: