XB-CLONE-2549099
Clone Name: IMAGE:8902913
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Gene:
fam43a
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC157653 [+]
GCAAGGCGTACGGGAGGGAGGAGCGCATTGTACATGCAATGATCGCTGCCAGGCGCTGTGTACTGACAAATCCCTGCTGCTCCCGCTACTCAGCACAATG
CATTAAGTGCGCTCTGAGTGCGCCCAGCGCTTCATTCTCCGCAGCTGTAACTTGCACAGCGACATGCTGCCCTGGAAGAAAAGCAAATTCGACCTGATCG
AGGAGGATCCGAAGCAGGCGAAGCAGAAGGGCTATGCAGTGAGTCTCAACTACAGCGCCCTCACCTCCTTCGCCAAGTCGTGTCCGGAGGGGGCCCTGAC
TCGGGTGGGCAGCATGTTCAAGTCCAAGAGGAAGAAGATCAAGATTACGGGGGAAGACCCTACATACACAGTGCTGTACCTAGGCAACGCTACCACCCTG
CAGTCCAAGGGAGAGGGCTGTACGGACCTAGCAGTGAGCAAGATCTGGAGCAAAAGCGAGATGGGCAAGCATGGCACCAAGATGAAGCTGACCATCAGCT
CGCAGGGTATCCGCATGGTGCATGTGGAAGACAAAGCCAAGCGACCCGGGCACCTGTATTTGCTGCACCGAGTTACCTATTGCGTGGCCGACCCCCGGCT
GCCCAAGATATTTGCCTGGATCTACCGCCATGAGATGAAGCACAAGGCGGTGATGCTGCGCTGCCATGCAGTGCTGGTGTCCAAGCCCGAGAAGGCGAAA
GCCATGGCACTGCTGCTGTACCAGACCTCCGCCAACGCCCTGGCTGAGTTTAAGAGGCTGAAAAGGCGAGAGGATGCCCGGCACCAGCAGCAGCAGCTGA
TCGGGGAGCAGAGCATTCCCCTGGTGCCCATGAGGAAGCTCCTGAACGGCCAGTGCTGCTATAAACCCCCTGTGGAGCGGAGCCGGAGCGCTCCCAAGCT
GGGCTCCATCACGGAGGATCTGCAGGGGGAGGAGGAGGAGGAGAGGGCTATGAGCTTCGAGTGTGAGGATGTGCTGGATACCGGGGGTGGAGGGGAGGGG
GGCGAGGAAGAGGAGGAGGAGGTGAAGCGGGAGCTGTCACAGCTCATCAATGATCTCGGGGAGATGAGCCTGGGCAATGACTTGCAGACTCTGAAGGCGG
ACCTGAGGGTAACCAGGCTTCTGTCCGGGGAGAGTACGGGCAGCGAATCGTCTATAGAGAGCAACCAGGATGCTGGCCCCATCACTAACGGGTTCGAAGA
GTGCAGAGAACCAGAGACTGTATGAGTAGGGCCACAGGGGCCCAGGGACTGTCTCATACAGACTCACTGTGACCCATTTAAAGCCAGAGGCTCTGCCCAG
ATCAGTGGCACCTGGGGGGATTCATTTCTTTCAGCAGGGGTGCCAACCTGGTCCTTTCTTTTCATCTTCAGAACAGACCACTAACATGCAGTTTCCAATA
GGTGATTTAATTTAAAATGACCTGGTACCATATTTTATTGAGATTTGATTCCCTTGTGGCACCTCTATCCTACAGGAAATGCCATTTGCCATGGAACTGA
AGGAGCCCATAGAACCTGTGTGATTGTCAGGATCCTTATTGCTGATATAGATTGGGGTTCACCAACTAGCAGCCCTCTGCTTGTATGAATTATACCCAGA
CTTAAGCTGTTGGGGGTATCAGGTGGCCAGGCATCCTTGAGTAGCTATAATGCCTTGCACTTAATGTAATTTTGTTAAAATGAATGCTGAATTATGAAAC
TTGAGGTGGTGGAGAGGAAAGGGAGCACCAGTGGAATACATGCTGACAACAGTATGGGTGTATGCAAGGGATCTATTTATAATGGGGAGGGGGCAAGGTG
AATGCCATGAAAATAGAAACCAGGTTCCGTTACTGGCCAGTAACAGATTTGTGATGTTAGTGAGTGTCAGATGACTGTACATTGCACTGTGTTGCATATG
TTTCTGACACTCTGGTTTAACTACCCTTTGTAGCAGTGAATGTGTTGAGGTGAATTAATGTGTAAGCCCCTATGTCTTACAGAATATAGAGTCTTTATCT
ATGGGAAAAATCTAATTATAATATCACCCAAAGCTATTACATGTAGTAAAAACTGGTTCACTGCTATAGATTCCAGGGTCACAGGGGCCAAAGGGCGAAG
AGACAATCCTGCACAGATGTCTCCAAGAGCTTATGCAAATAAATAAGGTTTAGAGGAGTACTCTTAGCAAATTGTCTCTTCACCTTATGATCAATATACC
CCTGACTAGTGTGGTGAAGATGCAGAGGATTTGTGGAAGTGATTATAAGATGTACATGGCTTACATCTCCTAGTAAGGAATGTGTTACAGCAAAGTCATG
GATAACTCAGATGGTGTGTGCAATAATTATTTTGGGGCCTAATGCAAGTTGTTTGAACCTGTGGTGCTTATTCACCAGAGAGCAGTATTATTTCTAGCAC
ATCCTATAATTACTGTAATGAAACCTTGTTTCAGAAAAAAATGCCCTAAGACCTAGCTTATGTTACAGGCTAGAGCAGAGCAGACTTGCTACTTGGGGCA
TTATGAACCTATATAGTATAACTACTTATATAATTGCCCAACCTAGTTATTTAGTTAATGGATTTGGCAGGGCTGAGGGCTTAACGTAAACAGCCTTGTG
TTTTATTGAGGATCTTTGTGCCATTCTTTTGAAAGAAACCCCAAGGCTAGATGGGATTAAGACTAGCTTTATATGCCTAGACAGAGTACCCAAGGCATAC
ACGCACATGTAAGCACTTTCTTTAGGGTTCGGCTGCTCACACAAATAGGAAAAGTGGGCTGCAAATTACAATCACATGGGATAATGTGAGATTTTAAGTG
CAATGGCAGGGAGAATAGGCAGTGCCACTTGTAGATATACTGATTTTGCACACTTCTTTCTGTGCTAAGGTTAGTGGCAATAGCTTTCTTTTGGCAACAC
ACACTCCAATATCTTTTTCTAATATCTTGTATCTGGGACATTGGTGTACATGTAACGATGGTATGTTATAGCACTGGAATGGCCAGACTCTTTCATTGTT
TAGGTAAACCCTTAGCCATTGGGGAAAGTTCACTGAGATTCATGCAGTGTAGAGGTTTAACAGCATACAGTGAAGCACCCATGTAGCAATACATGCACTC
ACTATGGTGAATGGTACAATTAGAGCTAACTAAGTAAAAGTGGGCTTGTGTCACTGTACTGATGGTATCCCATCACATGGTTTCTGATTGTGATCTATTA
TCCATTTAGCAGTGCTGACTATACATCATTACCTATTCAATATCTCTGCAGTAATCTACAGAATTGATGTCCATGCCTTAAACTTGTATTTTATAGTACT
ATCCATCTAATTGCACTTTCTATCTTCTCATTTGCTACTAAACCTCATTCCAGTCATAAATAGGCCTTGCAAGTCCACACCTTGGCCCTTTAGGCTTCTA
AGACTGCAGTAGTTGGGGAGTCGCAGGTTGCACATCCCCTTTCTAACTTTATTCCCCCTCATTTATTTGGATGGGTAAATGTAGTGTGATTACTGATATG
ACAGTGTTTCCCTAGAAGTCATATTATGTATAGTAAGGGTTGAAATTAACAGCACAATAAGGCAACACATTGAGAGAGGGTGGCAAATCCTGATTGGCGG
ATTTTATACATTCAAACACTACGACTGGCCATACGTTTTACTTCCAAAACAAGCTGCAGTACATGTGCCTGCATTGGTGCCATTGTGCCCTGTAATGCAT
TGCAAATGTCAGGGGAAAAAGTCAATGTTTCCATTCACATGCACAGGCTTGGGATCTATCTTTATACCTCAGAAAAGCACCATCTAACAGCCAATACTCC
ACTTGTAAATCACCCTGGAGAATCTGGTACCTTATTATGTTTACACTTTGTTTTTGGAACCAGCCCAAAGGGATCACTTCCTGTTTCGGTCTCATGACAT
TCCATGATATTTTTTTTTTTTAATACTTGCACTGTTGTGAAGTACTTTGCCAGTGTTTCATAAATGTAAATAGATATATTTTTTTGTACATAAATCACAT
AAATATAAATGTTATCTTTGCTGTGCAAAAGAAAAATTGTTGTTGTTCACCTGTAAATATTTTGTGTATATAATTTCTTTTTGTTACTTTTTAAAGGACA
TCACTGTTCTGATGTTTGTTTGCACACATGGAAAATAAAATAAATTAATATAAAAAAAAAAAAAAAAAGAAAAAAAAAAAAAAA
5' EST: EL800701 [+]gcaaggcgtacgggagggaggagcgcattgtacatgcaatgatcgctgccaggcgctgtgtactgacaaatccctgctgctcccgctactcagcacaatg
cattaagtgcgctctgagtgcgcccagcgcttcattctccgcagctgtaacttgcacagcgacatgctgccctggaagaaaagcaaattcgacctgatcg
aggaggatccgaagcaggcgaagcagaagggctatgcagtgagtctcaactacagcgccctcacctccttcgccaagtcgtgtccggagggggccctgac
tcgggtgggcagcatgttcaagtccaagaggaagaagatcaagattacgggggaagaccctacatacacagtgctgtacctaggcaacgctaccaccctg
cagtccaagggagagggctgtacggacctagcagtgagcaagatctggagcaaaagcgagatgggcaagcatggcaccaagatgaagctgaccatcagct
cgcagggtatccgcatggtgcatgtggaagacaaagccaagcgacccgggcacctgtatttgctgcaccgagttacctattgcgtggccgacccccggct
gcccaagatatttgcctggatctaccgccatgagatgaagcacaaggcggtgatgctgcgctgccatgcagtgctggtgtccaagcccgagaaggcgaaa
gccatggcactgctgctgtaccagacctccgccaacgccctggctgagtttaagaggc
3' EST: EL800702 [+]tttttttttttttttctttttttttttttttttatattaatttattttattttccatgtgtgcaaacaaacatcagaacagtgatgtcctttaaaaagta
acaaaaagaaattatatacacaaaatatttacaggtgaacaacaacaatttttcttttgcacagcaaagataacatttatatttatgtgatttatgtaca
aaaaaatatatctatttacatttatgaaacactggcaaagtacttcacaacagtgcaagtattaaaaaaaaaaaatatcatggaatgtcatgagaccgaa
acaggaagtgatccctttgggctggttccaaaaacaaagtgtaaacataataaggtaccagattctccagggtgatttacaagtggagtattggctgtta
gatggtgcttttctgaggtataaagatagatcccaagcctgtgcatgtgaatggaaacattgactttttcccctgacatttgcaatgcattacagggcac
aatggcaccaatgcaggcacatgtactgcagcttgttttggaagtaaaacgtatggccagtcgtagtgtttgaatgtataaaatccgccaatcaggattt
gccaccctctctcaatgtgttgccttattgtgctgttaatttcaacccttactatacataatatgacttctagggaaacactgtcatatcagtaatcaca
ctacatttacccatccaa
Library:
Description: The library was made from dT primed cDNA and cloned into vector pCS107. PolyA RNA were primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3') and digested with NotI. cDNA was size selected using 1.1% agarose gel electrophoresis (>0.6kb) then ligated into NotI and SalI digested pCS107 vector. Primary library, non-amplified. Library constructed at the DOE Joint Genome Institute (Walnut Creek, CA) as part of the Xenopus Gene Collection project.Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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