XB-CLONE-2541635
Clone Name: IMAGE:8910179
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Gene:
mybpc3
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC155456 [+]
AACCCGGTACTGGTCTGGAACTACTGCTGGAAATTGTCTAGTTAGCATTTGGGTTCTTCCGCCAAGCAGGCTGAGCTAGGATGCCTGAGCCTGTCAAGAA
ATCAGCGTTTACCAAAAAGCCAAAATCTGAGGAGGTCTCAGTGGGTGGAACTGTTACCTTTGCGGCAGAGACAGACAAACCTGGTCTGAAGGTGAAATGG
CAGAGGAACAATGTCGACATCACTGCAAATGAGAGGTTTGCCATAAAGTCTGAGGGCAAGCAGCACTCACTGACCATCAGCAATGCCACCGCAGAGGATG
AAGTGGTCTATGCCGTCATTGCAGGCACCTCGAAGGTCAAGTTTGAGCTAAAAGTCAAAGAACAAGAAAAAACTGAGGAGGCTCCTGCCCCAGCTCCAGC
TGCTCCTGAAGCTTCTGAGGCACCTGCTCCTCCAGCACCTGACAGCTCAGCTCCACCTCCCACTGAAACTGCTGAGGGAGAGGCTAAACCCCCAGAGTCT
GCTGAAGCTACTGCTATTCAACCTGAACCCCCTAAGGATGAAATTGGACTGTTCCTGGAAAGACCAAAGGATGCGGAAATCACTGTTGGTTCCAATATCA
AGTTTACAGCCAGAATCGCCGGTGCCAATTTGATTAAGAAGCCGGTAGTCAAATGGTTTAAAGGCAAATGGATGGACTTGACCAGCAAAGTTGGCAAACA
TTTGCAGCTCCATGAGATTTATGACAGGAACACCAAGATATACACCTTTGAGATACAGATAATCCAAGCCAAAACCACCTACGCCGGGGGTTACCGATGT
GAGGTTTCCTCAAAAGACAAGTTTGACAGCTGCAATTTTAACTTAGCAGTTCATGAGGTATCTACATCAGGAGAGGTAGACATTCGTGCAGCATTCAGAC
GCACCGGAGATGGAGCTGAGGAAGCAGGGGAATTGGATTTTAGTGCCTTGCTTAAAAAGAGAGACAATGAGCCAAAGCAGGGCGCCGAGCCCGATGTGGA
TGTGTGGGATATCCTCAAGAAAGCACCCCCTTCTGAATATGAGAAGATTGCCTTCCAGTATGGCATCACTGACTTGAGGGGCCTCTTGAAAAGACTCAAG
AAGATGAAGAAGGAAGAGAAGAAGAGTGAAGCTTTCCTTAAGAAGATGGATCCTGCATACCAAATAGACAAAGGTCAGAAGATGAAGCTGGTCGTAGAAG
TGGCCAATCCAGATGCAGAGGTCAAATGGCTGAAAAATGGCCAAGAGATCAGAGTTAGCGGGAGCAAGTACATATTTGAGAGTATTGGGAACAAGAGGAT
TCTCACCATCAATAACTGCTCGCTGGCAGATGACGCTGCCTATCAGTGTGTGATTGGGGAAGAGAAATGCTTCACTGAGCTCTTTGTGAGAGAGCCTCCC
GTTCAGATCCTCCATTCCTTGGAAGATCAGATGGTGATGGTGGGAGAGAGAGTGGAGTTTGAGTGTGAGGTGTCAGAGGAAGGCGCTCAAGTGAAATGGG
AGAAAGATGGCGTGGAGCTGACCAGAGAGGAAACCTTCAAGTATCGGTTTAAAAAGGACGGTAAAAAGCATTTTCTGATCATTAATGAGACAACCCTGGA
AGACGGGGGCAATTACAAAGTCAAAACCAACGGAGGGGAGTCAGTGGCTGAGCTGATGGTACAAGAGAAACAGTTGGAAGTTCTACAAGATGTGGCTGAT
TTGACTGTTAAAGCCAAGGAACAAGCGGTCTTCAAGTGTGAGGTCTCCGATGAGACGGTGACAGGTATATGGGTGAAGAACGGAAAGGAAGTCATTCCTA
ACAACAGAATCAAGATCACCCACATTGGAAGGGTACACAAGCTGACCATCGATGATGTTGTCCCACAGGATGAGGGAGACTACTCCTTTATCCCTAATGG
ATTTGCCTTCAGCCTTTCTGCAAAACTACACTTTATGGAGGTGAAGATTGACTTTGTTCCAAGACAAGAACCCCCTAAGATCAAACTGGACTGCATGTCA
CAGTGCGCGGACACCATCATTGTCGTTGCTGGCAACAAATTACGTCTTGACGTCCCCATATCTGGGGATCCAGCCCCCACTGTGGTGTGGATGAAGGGGG
ATAAGCTAATTTCAGAGGTTGATGGCAGAGTCCATGTGGAGACACATGCAGACCATTGTGTGTTCATCATTGAGGGGGCAGAGAAGTCAGATGAAGGTGC
CTACACCGTACTGGTGAAGAATGAAGCTGGAGAGGACAAAGCTAACATCAATGTAAAGGTTATTGAGGTCCCAGATCCACCTGAGTCCCCCAAGATATCA
AACATTGGCGAGGATTTTTGCACAGTACAATGGGATCCACCCAAGTATGATGGAGGCCAACCAGTCTTGGGTTATATTTTGGAGAGAAAGAAAAAGAAGA
GTTACCGCTGGATGCGGCTGAACTACGACCTGGTCAAGGAATTAACCTATGAATCTAAGAGGATGATTGAGGGAGTAGTATATGAGATGCGCATATATGC
CGTAAACTCCATTGGCATGTCCCTCCCCAGTCAGCCTTCACAGCCCTTCATGCCTATTGCTCCCACCAGTGAGCCCACCCAGCTGGTGGTGGAGGACGTC
AGTGACACCAGTATAAGCTTAAAGTGGAGACCCCCAGAGCGCATTGGCGCAGGAGGACTCGATGGCTACACCGTGGAATACTGCAAGGAAGGGTCCACAG
AGTGGGTCCCAGCACTGCAAGGCCTCACTGAGAGAAATTCAGTCATGATCCGTGACCTTCCCACCGGAGAGAGACTCACCTTTAGAGTGCGTGCCATCAA
TCTGGCAGGACCCAGCGAGCCCTGTACCATGAAAGAACCTGTGACCATTCGCGAAATCATGCAACGTCCAAAGATCTGGCTTCCACGATACCTCAGACAA
AAACTGATCAAGAAAGTTGGCGAAACAGTGAACATCGTCATTCCCTTCCAGGGTAAGCCACGCCCAGTGGTGACCTGGCTGAAAGATGGACAGCCTGTTG
ACCCAAAACAAGTCGGAATCCGCAACAGTGAAGCAGACACCATTTTGTTTATAAGAAAGGCAGAGCGAGACCATTCTGGGGAATATAAAGTTCAAATCCA
GATTGAGAACTGCGAGGACTCGGCCACCATCTGCATCCAAATCGTAGATAAACCAAGTGCCTCACAGAAATTAAAGATTGTTGAAATCTGGGGATTCAAT
GTGGCACTGGAATGGGCTCCTCCCCAAGATGATGGAAACACGGAAATTACTGGATACACTGTCCAGAAAGCAGATAAAAAGACCATGGAATGGTTCACAG
TCTTCGAGCACTATAGGCGAACACACTGCGTGGTGTCCGATCTGATCATGGGCAACGAGTATTACTTCCGAGTGTTCAGTGAGAACATGTGCGGCCTGAG
TGAGAAACCTTGCACTACAAAGAACAGTGCCTATATCCAGAAAACCGGAACTGCCTACAAACCTCCCACTTACAAAGATCATGAGTTTGATGAGGCCCCT
AAGTTTACTCACCCACTGAATGACCGGTCTGTTGTCGCCGGGTACAATGCCACCCTCAGCTGTGCCGTACGAGGCATACCCAAGCCTAAGATCACATGGT
ATAAGAATAAGATGGACCTGTCCATGGAAGCCCGCTATCGCTCCTTCAGCAAGCAGGGGGTGCTCACATTGGAAGTGAGGAAGCCAAGCCCATTCGATGG
TGGCCTCTACACATGCAAAGCAGTCAATGAGCATGGGGTGGCAGAAACTGAATGCCGGCTGGAGGTGCGAGTTCCTCAGTAACAGTCCCATGGGGTGATT
TTAGGCGACTGAAACAGAGGAGACAGAGGAGTTGAGACGCAGACTGTAAAATTCATAGATGAAGCAAGTTCAATCTGTATTAGATATATGTAAAAGAAAC
CCACAGACACACAGCAATATCATCTCTAGATTCACAGAATTACAAAGAGCACTGTGCTGCCTGCTACGATCCTTGCCTTCATCTCACGCGGCAGAGGCTA
TCTCGGTGTGGATATTATTTTTCGCAGTATTGGCAGGAACAGATTAAATGCCAGGGGGATAAGGGCGTTGCCTGTATGATATATAGAATATGATATGTGT
AAGTGATTTTGAAAGGTAAAAAGAAGTAATAAACGTTCAGCTTGTTGAAAAAAAAAAAAAAA
5' EST: EL805597 [+]aacccggtactggtctggaactactgctggaaattgtctagttagcatttgggttcttccgccaagcaggctgagctaggatgcctgagcctgtcaagaa
atcagcgtttaccaaaaagccaaaatctgaggaggtctcagtgggtggaactgttacctttgcggcagagacagacaaacctggtctgaaggtgaaatgg
cagaggaacaatgtcgacatcactgcaaatgagaggtttgccataaagtctgagggcaagcagcactcactgaccatcagcaatgccaccgcagaggatg
aagtggtctatgccgtcattgcaggcacctcgaaggtcaagtttgagctaaaagtcaaagaacaagaaaaaactgaggaggctcctgccccagctccagc
tgctcctgaagcttctgaggcacctgctcctccagcacctgacagctcagctccacctcccactgaaactgctgagggagaggctaaacccccagagtct
gctgaagctactgctattcaacctgaaccccctaaggatgaaattggactgttcctggaaagaccaaaggatgcggaaatcactgttggttccaatatca
agtttacagccagaatcgccggtgccaatttgattaagaagccggtagtcaaatggtttaaaggcaaatggatggacttgaccagcaaagttggcaaaca
tttgcagctccatgagatttatgacaggaacaccaagatatacacctttgagatacagataatccaagccaaaaccacctacgccgggggttac
3' EST: EL805598 [+]tttttttttttttttcacaagctgaacgtttattacttctttttacctttcaaaatcacttacacatatcatattctatatatcatacaggcaacgccct
tatccccctggcatttaatctgttcctgccaatactgcgaaaaataatatccacaccgagatagcctctgccgcgtgagatgaaggcaaggatcgtagca
ggcagcacagtgctctttgtaattctgtgaatctagagatgatattgctgtgtgtctgtgggtttcttttacatatatctaatacagattgaacttgctt
catctatgaattttacagtctgcgtctcaactcctctgtctcctctgtttcagtcgcctaaaatcaccccatgggactgttactgaggaactcgcacctc
cagccggcattcagtttctgccaccccatgctcattgactgctttgcatgtgtagaggccaccatcgaatgggcttggcttcctcacttccaatgtgagc
accccctgcttgctgaaggagcgatagcgggcttccatggacaggtccatcttattcttataccatgtgatcttaggcttgggtatgcctcgtacggcac
agctgagggtggcattgtacccggcgacaacagaccggtcattcagtgggtgagtaaacttaggggcctcatcaaactcatgatctttgtaagtgggagg
tttgtaggcagttccggttttctggatataggcactgttctttgtagtgcaaggtttctcactcaggccgcacatgttct
Library:
Description: The library was made from dT primed cDNA and cloned into vector pCS107. PolyA RNA were primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3') and digested with NotI. cDNA was size selected using 1.1% agarose gel electrophoresis (>0.6kb) then ligated into NotI and SalI digested pCS107 vector. Primary library, non-amplified. Library constructed at the DOE Joint Genome Institute (Walnut Creek, CA) as part of the Xenopus Gene Collection project.Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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