XB-CLONE-2525220
Clone Name: IMAGE:8855504
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Gene:
gpr107
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Species: Xenopus tropicalis
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Sequence:
5' EST: EL818240 [+]
gatgctgatgtggttgcgactagttcctagctaaacatggcgcttcggagtggcggaggattaggttgctgggcgtcgggcttggtattgtacgggttac
tgtttctgctctttcccgtttcccgggctcggttccaccatctaatgctaaaggatgacgtcaggcagaaggtgctccttaacacgttcggctttttcaa
ggatggttccatgaacgtgaccattaacagcttctctgtaaaggacaataatgatttttccaatttggacagttccacctttggctttagcctggacaga
acgaggaatgatggcttctccacatacttggaccaggagcttcgtgcttgtatcctaaagagccccccaacggatgttcccattatgcttctcaagatgg
attttgccaagaaacttgtttttgtgcgcagcgccaaacatgatatgccgattgtctctgtctcggaaaaggttgaacagccacccaaaaacatctcaaa
agctgaagaacccgcgccgcttcctgccaagatgagccggcgagatacagctgaccaaaagaaagatgatgctgcggataaaaaagaaggcacggcggag
gctgcctcgaaggacgataaagatgccacgcaagaagccaatcagaagaaagtggaaaagaaagagaaagaacaaaagggtcccattgaatataatggca
ctatgcacgt
3' EST: EL818241 [+]tttttttttttttttggaaagcaaatacaattttttattcacaaaaaaaagtgtagatttgcaacttaaaaagtaactgtatcctgctcagtgcctctag
gtgccagccttctacagtaaagaacagtctcatgagtcgctgcagactgggccacacgaagagtcatagtcaccatcttgctctactactgtcaccatca
tgccctactactcacgctacgtcacactcttcctccccaatgtaactctctgcctatgaaggcattaaacatacggcagccacgggggatagttttaggg
tacagtccagggttggctgttctcctgtgggaaattacatgacttagtgggccataccgccaacactcccatgagcctcggcgagtcagccaatagcatg
tttgtcccatgcgtggtgtctttaattctattaaggatataaatgatttgcctgtccatcccctcccccaaaaggtgttaatataagccaataaatacaa
aatggaagtgaacccatagccttctcgtctctgtacatttttgggggcaaaaattccagaaaataaaaactgttaaaaaacaaaaaaacaaaaaaaacac
acatttcccctcaaagtcttccccatgaaaagcaa
Library:
Description: The library was made from dT primed cDNA and cloned into vector pCS107. PolyA RNA were primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3') and digested with NotI. cDNA was size selected using 1.1% agarose gel electrophoresis (>0.6kb) then ligated into NotI and SalI digested pCS107 vector. Primary library, non-amplified. Library constructed at the DOE Joint Genome Institute (Walnut Creek, CA) as part of the Xenopus Gene Collection project.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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