XB-CLONE-2523607
Clone Name: IMAGE:8856307
|
Gene:
atf2
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC155444 [+]
attgcaggtcagcatctcgatgacgctcagatttgctcatcaaaagtctcctctgagagctgtaacttattaaattaataactgagggaagatgtctctg
ttagtgcaggagcatcggacggtgcgctttgtgtttctgggcgcagccggggttggtaaaaccgccctgatcacccgcttcttgcaggatcgattcgact
ctaaatacacgcgcacggtggaagagcttcacgcgctcgagtacgacactgaaggcgctcgggtgcgcattgagatcttggatacgagcggaagttattc
ctttcctgcgatgcgcgctctttgcatccgaaccggggacgcgtttgcgctcgtgtacgcagccgacgagccagattccctggaggaggtgcagagactt
cgtgaggagattctggagctaaaaggagagagttttactggcatcacggtgatcgagaacaaagcggacctgtgcagccggagtcgccaggccactgcgg
aggcgatgcgcgcggtggaggaggactggggcgcgggcttcgtggaaacctccgcgcgcaccggcgacaatgtcacagctgtgtttcgggacttgcttca
acagatgaagttaccgagccgggtgagtccggcactgcgcagacggacacagacaatgtccagagagctcacagagaagcgggaaaagcccccaatgaag
aaaaacaacagctgcatcttgtcttgatggacatttatatgaaaatgtagttatgcatcatactgatgcttaagtgatctggatcaaatatagaccccaa
atgttttatgtgtgtttatgctttatcattatcatttaagggaattctctgaatatgtctggtctctctgagaactaaagtgatcttttgtgagtgaata
aatgcaaatatgttccagccaaaaaaaaaaaaaaaaaaaaaaaaaaaaa
5' EST: EL819286 [+]cagaaattggcaagtacctaaagaatcccaccgaagcatgtcagttccaagaagagctcatatagcgtctgtcaacgaggaactagatgtagcttgagtc
ggaacagaaattatttctgctttcagtttcatgttattataagtccctcacgcataagaccttagaaaggtaatacaaggacctaaggaatatgagtgat
gacagaccttttgtatgcactgcccctggatgtggccagcgttttactaatgaagaccatttggctgtacataagcataaacatgagatgacgcttaagt
ttggacctgctcgaaacgatagtgtaatagttgcagatcaaacaccaacaccaactcggtttttaaaaaattgtgaagaagttggtttgtttaatgaact
tgcaagtccgttcgaaactgacttcaaaaaggcctctgatgatgacataaagaagctgcccttagatttgtctcctcttgtaacaccggttataagaagc
aaaatcgaggagcctgtcatagtagaaacgacgcatcaagacagtccattacctcacccagagtctaccacaagtgacgaaaaggaagtgccattgccag
ccgcacaacccacatctaccattgtacgccctgcgtctcttcaggttcctaatgtgctgcttacaaatacagattcaagtgtcattattcaaccggcaat
accttcaccaacatccagcactgtaatcacgcaagcccctccagctagcagaccaatagtaccagttccagctccttttcct
3' EST: EL819287 [+]tttttttttttttttcgctgttaaaattctacttttatggccaaagtaaatgtacctgtagctgcacattgaaagaactgaggtcctcggctttcttctc
tagagactgcacccaaacttttctcttctgccggcacctggaagcagctgcgcggttccgttctaaaaacttcctccttttctcatctgggtcatcgctt
gctgcttttctccggcgcccccctgtgctttgagtttgtggagcggggggaacaggagatgccggtgtttctgtagtggatgttgcaggctgctgcagtg
aatgaggctgagcttcctctggctgactctttgcagttccgctgccttgtccctttgttgcatcgccatttgttacctgaggattctgctgttgagtcaa
tgcagcttttagtctcagttttgcctctgactggacaggttgtggggaagcaggccctgggatccctggaatagtaggcaccacggtgacgggtctaacg
agtgggactgaagcaggtatgtgtacattggaatttgtgattgaggcaggaatagcaacaggcatggtttgtccattaggaagatgtaacaatagaggaa
aaggagctggaactggtactattggtctgctagctggaggggcttgcgtgattacagtgctggatgttggtgaaggtattgccggttgaataatgacact
tgaatctgtatttgtaagcagcacattaggaacctga
Library:
Description: The library was made from dT primed cDNA and cloned into vector pCS107. PolyA RNA were primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3') and digested with NotI. cDNA was size selected using 1.1% agarose gel electrophoresis (>0.6kb) then ligated into NotI and SalI digested pCS107 vector. Primary library, non-amplified. Library constructed at the DOE Joint Genome Institute (Walnut Creek, CA) as part of the Xenopus Gene Collection project.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: