XB-CLONE-2518548
Clone Name: IMAGE:8858619
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Gene:
chac1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC167986 [+]
atcatccgccgaggacgaaaagcagcgcggcagcccggggtgcgcgaaaacaacggttaaaaggttattcgatccgcctttacagcaccgtatgattggc
cgggtagaggtttgttgataaacaggaggctggccggcgagctagcaacagctttttgtgttttattccgattgaaagagccgaaaaggccaagatggag
ttttaaggcaatgatgcggtaactgcacggcataggcatgcaccaggcaaagaaaagcgttttgcgagactgcaccatcacaactgctaagcagttgggc
tctgataccgtccgcggtatagccgtgagccaacccatacctcttctccttgataggaggaagctaacgctagctacctagcctgtggcctatccattta
gctgtttttacatcaaagcgcacactattaccttttctacactcataccatcctgtaaaagatgaaacctcaagacatcgtcgctggaaaatccagcttg
tggatcttcggctacggcttgctggtctggaagccagacttcaagtacaagaggagcaaagtcggttatattaaggggtacaagagacgcttctggcacg
gggataactttcatcgtggagatgatgaaatgcccggaagagtggtgacgctcatcgaggaggatgacgcgtgcacctggggtgttgccttcgaggtgac
tggctctcagatggaggagtctctcaagtacctgaacgtgagggaggcagtgagaggtggtcatctgacccgggctgtcgacttctttccgcgtggcacc
aatcagccaccagtccaggctctggtttacatcgccacgcctgacaaccccatttacctggggcctgctagcacggaggaaatcgcctctcagatcgctg
tgtgcaaggggaattcggggcacaacatcgagtatctgcttcgcctggcggagttcatgagagtcagctgtcctgatgtggacgaccctcacctgttctc
catcgaggcagctcttctcgccaccattagacccatattattggccgcatagaaacctgtagatcttctgtaaagcaatcaaataggtcaaaacagcatt
aaggttctaatatgaaagttaatgttgccgtccttaaggtgcgatttgtcattttaagtggctgttcagatattttattctccctataccaatttatcac
tgtaatacatcaataggtgctgatgttacgggaacatggtatttttagcctgtcaacatttgaagtctgtaatgtcaaaacatgagtactgtcagttatc
attgttttgtgctcaagtatgttgtgctgtggtacaaatgcatgcttgtgatgatgtattgccaaacttgtccagttcttaagctgtttatgttgtgaat
atgttcgcatggaatctcaagacactcccccttttttattttacaatttgcactaagcagaatactgttaagtcattacagttatttgcacattaaaacg
atcaacaaaaaaaaaaaaaaaaaa
5' EST: EL822569 [+]tccaatagtggactgatttggaagatgtggctgccgcagctcatgttggttgttagtgtttgcggtgcacggcagctagatcccacgcccgcatacaaca
atgaacgttttgtcacagatcttgtgaatgcacacaatgacattcggaatgaatttgggaaacaagcagcgaacatgctacacatgtcatgggatgtagg
attggcaaaacttgcacaggcatggactattaattgtaaaaaagtaccaaacccacacctaaacaaagaaagcatctatcccagatttaaacagattgga
gaaaacctttacatgggtccatccattgacatatttaaaatagttacgaattgggggcttgaagggaacttctatgatttgaaaaataattcatgtcagc
ctggtaaagactgtagccattttactcagattgtttgggcaaatacttacaaagtaggttgcggagctgcatactgtgcacataaggttgcttatgtagt
ctcttgcacttatggaccacgtggaaatttgctgggacaagtccctttcatactgggtgtgaaatgttctaaatgtggaggggaaaaatgcaatgtggct
tcatgtggtaatccatcgcgagacgaa
3' EST: EL822570 [+]tttttttttttttttgaatgcagagaatattttaatatccttttaaaataaatcttttaaaagttattagttttatatgaacacgttaaaatattgctta
ttgcttaacccaaaatattacataaatatgttttaaagcattagtaagattggcataacatatattaaaacaattaaaatttaaaagcagtgcaaaggaa
aagagataataggaacaggacagtaggatgacacaggccctgtgtcattgttgtattcagtatcttgggctactggtctggccctttactaccaattagc
ttgtggataaattcatgcacatagcaccaaacaaggctacacatgtttttaaataaacatcttttgatctagatgtgtgatatctaagagtagggtaacc
agcaaaccatcaacaagtgcaattggcacttggtttgtatttgcaaagttgctgttctttagtattataataaaagtagatgatgctgagccttaggttg
gccttgaaaaaatcacctttgttttatcagatatatgtttgattttggaatctggcatgcaaaaatgctaatcaccaaagcctga
Library:
Description: The library was made from dT primed cDNA and cloned into vector pCS107. PolyA RNA were primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3') and digested with NotI. cDNA was size selected using 1.1% agarose gel electrophoresis (>0.6kb) then ligated into NotI and SalI digested pCS107 vector. Primary library, non-amplified. Library constructed at the DOE Joint Genome Institute (Walnut Creek, CA) as part of the Xenopus Gene Collection project.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: