XB-CLONE-2511449
Clone Name: IMAGE:8914260
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Gene:
tmco6
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC161230 [+]
aaacttcctgtttgtcactttccaaggacccctctgctgagaaaatgtcaagactttcgtgtctgttctactaaaaatgtggagaaggaggaaacagaga
gacacatcagtcccagtactggaagagttaagagcacaatggagggaacgggagacagctctgaggaaagcaaggagagaagagcagctcatcagtaaga
gactgttacgggacttaactgaagaaggagctcatgggagtgatgatcatatgcttgaagagaatgcccagtcacatttcatgtctgtggagcagattgc
aaaattaatcgaggatctccaacgtgagccagaacagatgattacgcctcttactgctcttcgacacagtctccgtagaaatgatgtgcgtctaatgttt
accagggtggaagacagcatgagggttttggtcgcactgttcacatgcccctttactgatatccagatggaggcagccaggtgcttgcatgaactgtctc
attcaaatgaactgcaagtatcaatggcatgtctcccagcaacaccatacctcctcacatatctgtcgggatatagtcaacagtttacagtattatgctt
gtacaccttgggtaatctcattgtggatagtgaagaagtaaggaaccggttgcttctccaaggagtcatcccaacatttgccaaatgtactcagtctcca
cacatggatgtacaggaagccctaggctatgcattttctcagctattgcagtcaaaagaagcacctgagaagattattccaatggttctagaatctggcc
taacacaggatatcctaagaatactgctgtcctgcactgaagagtggtttggagttgctattgagatggcttggtgccttcattacattgttagcagcca
cgtgaacaatgcattgctgatatcccaaggtttggtgtctaaattagtcccgtttctaatagaaaaagctgcacttattacaaagcaagaatctttggat
cttgaactgctgatctgcccagtgatacgttgtttaggaaacctgcttggagaagtggactcatctggcacgaaggttgaggtccaagatgggcgtctgc
taatagctttgttcgttttcatacaacatttcaaggatgaacatccatttatggtacgggagtgtctctgggctgttaacaatcttacagtacaagatcc
tgtttggacctcagctttactaaacttcaacttattgcctgccctgctacaactgctggcccattcaaaagatgtcttcctgatgattgccacaattttg
cataacatcgcagatctggggttgggttattgtcagcaactgagggaaaaaggagttctgccgtgtttaaccccagctctgcgtgacgacgacatacaag
tggcaaggataagcctagaattactgaataagcttttaacatactgtccagatgttgctggagactttttccataactccggtctacagatcattgagtt
acataaggataagcctgatcttcagcagcaagttcaagcagtctggaacaaatatcaagaactgataaaaggaaccagtggaccccccagtatggactaa
agaacagaaatcatatgaaatggacaactggcattttagcattgctttttgcggatgcattaatatttatctttctaataaaataagcatgaattgcaaa
aaaaaaaaaaaa
5' EST: EL827177 [+]aaacttcctgtttgtcactttccaaggacccctctgctgagaaaatgtcaagactttcgtgtctgttctactaaaaatgtggagaaggaggaaacagaga
gacacatcagtcccagtactggaagagttaagagcacaatggagggaacgggagacagctctgaggaaagcaaggagagaagagcagctcatcagtaaga
gactgttacgggacttaactgaagaaggagctcatgggagtgatgatcatatgcttgaagagaatgcccagtcacatttcatgtctgtggagcagattgc
aaaattaatcgaggatctccaacgtgagccagaacagatgattacgcctcttactgctcttcgacacagtctccgtagaaatgatgtgcgtctaatgttt
accagggtggaagacagcatgagggttttggtcgcactgttcacatgcccctttactgatatccagatggaggcagccaggtgcttgcatgaactgtctc
attcaaatgaactgcaagtatcaatggcatgtctcccagcaacaccatacctcctcacatatctgtcgggatatagtcaacagtttacagtattatgctt
gtacaccttgggtaatctcattgtggatagtgaagaagtaaggaaccggttgcttctccaaggagtcatcccaacatttgccagatgtactcagtctcca
cacatggatgtacaggaagccctaggctatgcattttctcagctattgcagt
3' EST: EL827178 [+]tttttttttttttttgcaattcatgcttattttattagaaagataaatattaatgcatccgcaaaaagcaatgctaaaatgccagttgtccatttcatat
gatttctgttctttagtccatactggggggtccactggttccttttatcagttcttgatatttgttccagactgcttgaacttgctgctgaagatcaggc
ttatccttatgtaactcaatgatctgtagaccggagttatggaaaaagtctccagcaacatctggacagtatgttaaaagcttattcagtaattctaggc
ttatccttgccacttgtatgtcgtcgtcacgcagagctggggttaaacacggcagaactcctttttccctcagttgctgacaataacccaaccccagatc
tgcgatgttatgcaaaattgtggcaatcatcaggaagacatcttttgaatgggccagcagttgtagcagggcaggcaataagttgaagtttagtaaagct
gaggtccaaacaggatcttgtactgtaagattgttaacagcccagagacactcccgtaccataaatggatgttcatccttgaaatgttgtatgaaaacga
acaaagctattagcagacgcccatcttggacctcaaccttcgtgccagatgagtccacttctccaa
Library:
Description: The library was made from dT primed cDNA and cloned into vector pCS107. PolyA RNA were primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3') and digested with NotI. cDNA was size selected using 1.1% agarose gel electrophoresis (>0.6kb) then ligated into NotI and SalI digested pCS107 vector. Primary library, non-amplified. Library constructed at the DOE Joint Genome Institute (Walnut Creek, CA) as part of the Xenopus Gene Collection project.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: