XB-CLONE-2510469
Clone Name: IMAGE:8915122
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Gene:
cfap52
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC154922 [+]
CGGACGCGTGGGCGACGGGGGAAGCGCAGAACAGTGATGGCTGAACAGGGGGAAGACAACCGGCTGGAGCTGCAGGGAATCATCGGGTTTAACGGACATG
TTCCGTCCGGCCTGATCAGTCACCCCGACAGGGAGCACCTCATTTACCCCCTGGGCTGCACCGTCATCATCGAAAACCTGAGCAGCCGCAAGCAGCAGTT
CCTGCAGGGTCACACCAACAATGTGTCGTGCGTGGCGGCGTCCCGGAGCGGCCGGTACCTGGCGTCTGGGCAGGTCACCTACATGGGATTTAAGGCAGAC
ATTCTCCTCTGGGATTACGGGAGACGCGAGGCCTGCGCCAAACTGACGCTGCACAAGGTCAAGGTGGAGGCTTTGGCCTTTTCTCCCAATGACCTCTACC
TGGTGTCCCTCGGAGGCCAAGACGATGGAAGCGTGGTGGTGTGGAATGTGGCAAAGCGAGAAGCCATCTGCGGCAGCCCAGCATCGTCCCTCAGCTCCGG
GCACGCCACCACCATCATCTTCGCCAACAATAGCGATGACGTCTTCATGACCGGAGGGAACGGGACACTTCGGGTCTGGGATGTGGATCTGCCCAACAGG
AAGATGCGCCCTACGGAGTGCCAGCTGGGCCAGATGAAGAGAGTGGTGACGTGCCTGCAGGTGGCAGAGGACGACAGCTGCTTCTACTGCGGCACCTCCT
CGGGGGACATCCTCACAATCAACACCAAGAGCAAGTTACTGAGCAGCTTTGCCCCGCAGAAGGGCAAGTTCAGCCTGGGGGTGACGGCTCTGCGGCTGCT
AAAGACGGGCGAGTTGCTCGTTGGTTCTGGCGACGGGAAGCTGGCACTCTGCAAAGGTCCGAACTACAAAGCGGCCAAGACTGTCCAGCTTCAGGGCTCC
GTCACTTCCATCTCCCTCCGAGGCCAAGGGCACCAGTTCTTCGTGGGCACAGGAGGGGCACAGATATACAGATTCAGTTACCCAGAATTCAAAGAGGAGC
TCATCGCCACGTGTCACAGTGAGGCCATCAACGACATCGCCTTTCCCTTTGGCACCTCAGAGCTCTTCGCCACCTGTTCCAAGAACGACATTCGAGTGTG
GCACACTGCGACCCACAAGGAGCTGCTCCGGATCACCGTGCCCAACATGACCTGCCTGGCCATCGACTTCATGCGAGACGGGAAGAGCATCATCTCAGCT
TGGAACGACGGCAGGATTCGGGCGTTTATGCCGGAAAGTGGGCGGCTGATGTACTGTATAGAGAATGCCCACAGCATGGGAGTGACGGCCATTGCTGCCA
CCAGCGACTGCAAGCGGATAGTGAGCGGAGGAGGAGAGGGACAGGTTCGGATCTGGGAGATCAGCAAAGAGACCCAGCGGCTGGTCCAGTCCATGAAGGA
ACACAAGTCTTCTGTTACCTGCATCAAGTTAAAGGGGAATAACCGGGAGTGTCTGACTGCCAGCTCTGATGGCACCTGCATCATATGGGACATTGTCCGC
TTTACACGACTCCAGATGGTCCTGTCCAACACGCTGTTCCGCTGCGTCTGTTATCACCCCGAGGAGTTCCAGTTTGTCACCAGCGGCACCGACAGGAAGG
TCGGATACTGGGAGGTGTTTGACGGATCGGCCATCAGGGAACTGGAAGGGTCCAAGTCCGGGGCCATTAATGGCATGGATATATGTGATCAAGGCGCTCA
CTTTGTCACAGGTGGCGACGACAAGCTGGTGAAAGTTTGGGATTACAACGAGGGCGAAGTGACCCACGTGGGCATCGGGCACAGCGGGAACATCACCCGG
TTGAAGATCTGCCCCCTTTCCAAGTATATCATTAGCGTCAGCACCGACGGGGCAATACTGTGCTGGGACTACCCCTATGGGCTCAGATAGTTTTTGCCCT
GGGTAATGCCCAATGGGCAGGGGGCGGCTGGGGATACAAATCTACTTCTCTTTAAGACTGACTTGGGCATCTCAGATTCTGTGGGTCCAACTGAGCACCG
GAACATGGACTGAACCCCCGGCGCTGCTGGGCTCAAGCAGAGGCTACTGCATGGCTAATAACGCACGGACTGCCCGAGCTTCTCATACTCGCCCCATGCT
CTTGTGTTGATGTCATTTTATTTTGGGAAAAAAAAGCTGAAATTCAATAAAAAAAAAAAAAAA
5' EST: EL827814 [+]cggacgcgtgggcgacgggggaagcgcagaacagtgatggctgaacagggaggaagacaaccggctggagctgcagggaatcatcgaggtttaacggaca
tgttccgtccggcctgatcagtcaccccgacagggagcacctcatttaccccctgggctgcaccgtcatcatcgaaaacctgagcagccgcaagcagcag
ttcctgcagggtcacaccaacaatgtgtcgtgcgtggcggcgtcccggagcggccggtacctggcgtctgggcaggtcacctacatgggatttaaggcag
acattctcctctgggattacgggagacgcgaggcctgcgccaaactgacgctgcacaaggtcaaggtggaggctttggccttttctcccaatgacctcta
cctggtgtccctcggaggccaagacgatggaagcgtggtggtgtggaatgtggcaaagcgagaagccatctgcggcagcccagcatcgtccctcagctcc
gggcacgccaccaccatcatcttcgccaacaatagcgatgacgtcttcatgaccggagggaacgggacacttcg
3' EST: EL827815 [+]tttttttttttttttattgaatttcagcttttttttcccaaaataaaatgacatcaacacaagagcatggggcgagtatgagaagctcgggcagtccgtg
cgttattagccatgcagtagcctctgcttgagcccagcagcgccgggggttcagtccatgttccggtgctcagttggacccacagaatctgagatgccca
agtcagtcttaaagagaagtagatttgtatccccagccgccccctgcccattgggcattacccagggcaaaaactatctgagcccataggggtagtccca
gcacagtattgccccgtcggtgctgacgctaatgatatacttggaaagggggcagatcttcaaccgggtgatgttcccgctgtgcccgatgcccacgtgg
gtcacttcgccctcgttgtaatcccaaactttcaccagcttgtcgtcgccacctgtgacaaagtgagcgccttgatcacatatatccatgccattaatgg
ccccggacttggacccttccagttccctgatggccgatccgtcaaacacctcccagtatccgaccttcctgtcggtgccgctggtgacaaactggaactc
ctcggggtgataacagacgcagcggaacagcgtgttggacaggaccatctggagtcgt
Library:
Description: The library was made from dT primed cDNA and cloned into vector pCS107. PolyA RNA were primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3') and digested with NotI. cDNA was size selected using 1.1% agarose gel electrophoresis (>0.6kb) then ligated into NotI and SalI digested pCS107 vector. Primary library, non-amplified. Library constructed at the DOE Joint Genome Institute (Walnut Creek, CA) as part of the Xenopus Gene Collection project.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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