XB-CLONE-2510092
Clone Name: IMAGE:8915403
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Gene:
cep70
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC155370 [+]
GCGGGGCACTGATAGAGGCGGCGTAGCCCTGGTAACGGACGGAGGCTCGGAGGGAATTTGGGCGCCTTTCTTGAATTTGTAGACAACTGTTAGTGCTCCC
CCTCCGTATTGTGACTTGGTTTCCTCAAGCGGAAAGATTGTACCGCCTGCCTCTGTACGGGAGACTTCCGGCTGCGATCATTGGCTTACACTGGCGGTGC
CAGAGGTTCTGGGACACAGAAGAAGATGGCAGAGCACCCACCGGCCTCAGAGGAAGATCCACTCAAGGAGGTTCTGTCCCAGTGGGAGAACATTAACAGA
ATTCTGAAGCGTCACGGCTGCAGCCCTGTCAGTGTATCCAAAGCCCCGGACAAGGGCAGTCTCTCAGGGGCGGTTCTGTTGGACCATCAGGCTTCCCTGG
CAGTGACCTCTGCACTCACATCCCTGGTACAGGACACAGAGAGACGGCAGAACCTGATCCACGGCCTCATCCAGTCCAATAACCAACTCAAGGAGGATGC
CCGCCAGCAGCAGGGCCGGGCCGCCCGACAGGAGCAGAGGGCCACTGACCTACAGAACATATTGGACAGTGTCAAAGCCAAAATCCGGGATCTGGAGGAC
GATTTTATCTCTAAGACCCGACAGCAGCAGAACCAAGTGGCGGAGCTTCTAAAGGAGAAACAGGCGGCCAATGAGCAGTGCCAGAAGCAGCAGGAGAAGT
TGGGGCAACTGGAGGAGAAGGTGGGGCAACTGAAGGAGCAGCTCTCCCGGGCACAGAGCGCAGAGGAGAAGAGAGGGGCCGCTCAGAGGAAAACCTTCCT
GCAGCTCCTGAGCCGGCTCCCCCGCAACAATAATGGAACGGACCAGCAGATACTGGATATCATTGGTGGATATGAGGGTCAGGTGACCCAGCTGCAGAAA
GAGCTCAGGAAATACAAGATGGCCGATGTGGAGGTGTCAGTCAGGGGCAGGAAGTACTCCCAGGAACCCCTAAATCTGGATACAACTCCCAATTACAGAG
CTTTACTGAAGTCGTATCAGGAACAAATGAGAGACGCCAAAGAGAAGAGAGAGCAGCTCCTGCGAGACATCTGCAACTTGCAGCGAGAACTAGAGGCCAG
ACCCGCCCCCCGGGAACTGAAACTCTACAAACAGCAGGTCCGGAAACTGGAAAAACTGCTCCGACAGAACAACATCAGTTTCCGTGGGACGATGCGAGAG
AAGAATGAGAGGAAGGCGCCGGTGCCGCCCAGTACCAGGGTAGAGGATGTGGATCAGCTCCCAACAGACGAGTGCCGGCGGTACCTACAGGAAATCTGCA
GGGAGCTCGGCGTCCGGGATCTCCGGGATCTTGTTCCTGTCTCTTCCGCTAAAGTGCGAGAGGCCGAAACGTGCGCCAAGTTACACAGGATCCTGAGTGG
GATCCGCTCGCTGCTCAGCAGCCCCCAGGCCCCCCAGCTCCTGTATAAGGGCAGTTCCCGAGGTCCCGGGAGTAGCGCCATCGATTCCAGGGAGGAGTCA
GACTTTTTGCATCTGCTGCCAACCATAGAGATGTGGGCGGGGCAGCTGCTGTCCCTCAAGGAACTGTACTGGTCTCTCCATAACCTCAGCGAGAAGGTAT
TGCCCCACCCCCTGCCCAGCCCGGTACCCGGGGGCCCCGCCAGCGTCCGAGTGGAGGATCTGCAGCTTCTACTGGATTCCATGATTGAGGATGTGGAGAG
CCAAAAGCAGGCCCCGGGCAGTCTCTCCCCACACACGCTGTTGGCGTTGGTCTCGCACTTCCAGAAGCTGTTCGACGCGCCCTCGCTGAGCGGAGTGTAC
CCCCGTATGAATGAAGTGTACAGCAGACTGGGGGAGCTGAGCAACATGATGAAGAGCCTGTGCTGCCTCCTGGGGATCGATGGCGTTGCCTCCTCCAGCG
CGGTGGTTAATGGCGTGTGGCGCCTGTGCCGAGACCACGAGGGCGGAGACAGCCGGAAGCTGCAGCAAATCCTCGGCACCTTAGACATTGACAGTGTCAT
TAACAAGATCCAGGAGCACGAGGAATTCTTTCCAGCATTTGAAGGGTTAATAAAAGCCCTGCTGAACCTCCTGGAAATTGACCAATTGGATGAGATCCTG
CCGGAGGTGCGCAGACTAAAGGCGCAAGTGGCACATTAGCGGTGGGGGGGGTGTTGTTTATACAGATGACTTTTTATTAATAAATATATTTATTAAGAAA
AAAAAAAAAAAAATAAAAAAAAAAAAAAA
5' EST: EL828058 [+]gcggggcactgatagaggcggcgtagccctggtaacggacggaggctcggagggaatttgggcgcctttcttgaatttgtagacaactgttagtgctccc
cctccgtattgtgacttggtttcctcaagcggaaagattgtaccgcctgcctctgtacgggagacttccggctgcgatcattggcttacactggcggtgc
cagaggttctgggacacagaagaagatggcagagcacccaccggcctcagaggaagatccactcaaggaggttctgtcccagtgggagaacattaacaga
attctgaagcgtcacggctgcagccctgtcagtgtatccaaagccccggacaagggcagtctctcaggggcggttctgttggaccatcaggcttccctgg
cagtgacctctgcactcacatccctggtacaggacacagagagacggcagaacctgatccacggcctcatccagtccaataaccaactcaaggaggatgc
ccgccagcagcagggccgggccgcccgacaggagcagagggccactgacctacagaacatattggacagtgtcaaagccaaaatccgggatctggaggac
gattttatctctaagacccgacagcagcagaaccaagtggcggagcttctaaaggaga
3' EST: EL828059 [+]tttttttttttttttattttttttttttttttcctaataaatatatttattaataaaaagtcatctgtataaacaacaccccccccaccgctaatgtgcc
acttgcgcctttagtctgcgcacctccggcaggatctcatccaattggtcaatttccaggaggttcagcagggcttttattaacccttcaaatgctggaa
agaattcctcgtgctcctggatcttgttaatgacactgtcaatgtctaaggtgccgaggatttgctgcagcttccggctgtctccgccctcgtggtctcg
gcacaggcgccacacgccattaaccaccgcgctggaggaggcaacgccatcgatccccaggaggcagcacaggctcttcatcatgttgctcagctccccc
agtctgctgtacacttcattcatacgggggtacactccgctcagcgagggcgcgtcgaacagcttctggaagtgcgagaccaacgccaacagcgtgtgtg
gggagagactgcccggggcctgcttttggctctccacatcctcaatcatggaatccagtagaagctgcagatcctccactcggacgctggcggggccccc
gggtaccgggctgggcagggggtggggcaataccttctcgctgaggttatggagagaccagtacagttcctt
Library:
Description: The library was made from dT primed cDNA and cloned into vector pCS107. PolyA RNA were primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3') and digested with NotI. cDNA was size selected using 1.1% agarose gel electrophoresis (>0.6kb) then ligated into NotI and SalI digested pCS107 vector. Primary library, non-amplified. Library constructed at the DOE Joint Genome Institute (Walnut Creek, CA) as part of the Xenopus Gene Collection project.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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