XB-CLONE-2506541
Clone Name: IMAGE:8917161
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Gene:
ankhd1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC157521 [+]
AGTTTACAAGAAGTTAATGGCTAGGAAACGGGTTGCTTTGCTTATTTTTTTAATTACTTCAGATGTCTGCTTTTGAGGAGTTCGCGTTTTGGAAGTCGGC
ACAGTAAGCAGACAAAGTTTAAAGACTCGTCGTTTCGCTCTTCAGATCTCGGTCTTTTTCCACGCTTGACGGGAGCTATGCAGAATGCTGTGACCGGGAC
GGACATGCTCACGGAAGGCTTTGAAGATGAGATCGACTCGGTAACTCCGCGCTCGCCAGTGACGGAGCTGGGAAGTGAAGCCGGTGTCGGACTCTCCGCT
GCCAAGGTTCGCCTTTTTGGGGGCGCGTCGACTGGAAACGAGAGACTGGATTTTAGGTTGGCTGCTGCCGCTGTGCTCTCCTCCGGAACTGGCTCTGGCA
GTGACGAGGACGAAGTGTCTGAAGTTGAGTCGTTTATTTTGGACCAAGAGGACTTGGACAATCCAGTGCTTAAAACTGCATCTGAACTCTTCCTGTCTAC
TGCTGCTGAAGGAACAGACTTTAGAACTGTTGATCCTGAGACACAGGCACGTCTAGAAGCTTTATTAGAAGCTGCAGGAATTGGTAAATTGTCCACTGCT
GATGGTAAAGCCTTTGCAGATCCTGAAGTACTTAGAAGATTGACATCGTCTGTGAGCTGTGCTTTGGATGAAGCTGCGGCTGCACTAACTCGGATGAGAG
CAGACAATACTCTCAGCACAGGACAAGTAGACAATCGGAGTCTCGCAGAAGCCTGTTCAGATGGAGATGTTAATGCTGTTCGTAAACTGCTTGATGAAGG
TAGAAGTGTAAATGAACACACGGAGGAGGGAGAAAGTCTTCTCTGCTTGGCCTGTTCTGCTGGATATTATGAGTTGGCACAAGTGTTGCTTGCTATGCAT
GCCAATGTTGAAGACAGAGGAAATAAAGGTGACATTACACCTTTAATGGCAGCTGCCAGTGGTGGTTTTGTCGATATTGTTAAACTGTTATTGGCTCATT
CTGCAGATGTGAATGCACAGTCATCGACTGGTAACACAGCGCTTACGTATGCTTGTGCTGGTGGCTTTGTTGATGTAGTTAAAGTTTTGCTGAAAGAAGG
TGCTAATATAGAGGATCACAATGAAAATGGGCACACCCCTTTAATGGAAGCAGCTAGTGCCGGGCATGTAGAGGTAGCAAGGGTCTTGCTAGAATTTGGA
GCAGGCATCAACACACATTCAAATGAATTCAAAGAAAGTGCACTTACCCTGGCATGCTACAAAGGCCATTTAGACATGGTTCGATTTTTGCTTGAAGCTG
GAGCTGATCAAGAGCATAAAACTGATGAGATGCACACAGCCTTAATGGAGGCTTGCATGGATGGCCATGTCGAAGTAGCGCGTTTACTGCTAGATAGTGG
TGCTCAAGTAAACATGCCTGCAGATTCTTTTGAGTCTCCTTTGACTTTGGCAGCCTGTGGAGGTCATGTGGAACTTGCTGCCCTTCTGATAGAAAGAGGG
GCTAATTTGGAGGAAGTAAATGATGAAGGTTATACACCTTTAATGGAAGCCGCACGAGAGGGCCATGAAGAAATGGTGGCACTCCTCCTTGCACAAGGGG
CCAACATAAATGCACAGACAGAAGAAACACAAGAAACCGCTTTAACTTTGGCGTGCTGCGGTGGATTTTCAGAAGTGTCCGATTTTCTCATAAAAGCAGG
AGCAGACATAGAACTTGGCTGTTCTACGCCGCTTATGGAAGCTGCTCAGGAGGGGCATCTAGAGCTGGTGAAATATTTGTTGGCAGCAGGTGCAAATGTA
CAAGCAACAACTGCTACAGGGGATACAGCTCTGACATACGCATGTGAAAATGGTCACACAGATGTTGCAGATGTGTTGTTGCAGAGTGGTTCCGATTTAG
AACATGAATCTGAAGGTGGAAGAACTCCACTAATGAAAGCTGCAAGAGCTGGCCATCTTTGCACAGTTCAGTTTCTCACTAGCAAAGGTGCTGACATTAA
TAGAGCTACTGCAAATAATGATCACACTGTGGTATCCCTTGCATGTGCTGGAGGTCATCTTGCAGTTGTTGAGTTACTTCTAGCACACGGTGCAGACCCA
ACTCATCGTTTGAAGGATGGTTCTACCATGCTGATAGAAGCAGCAAAAGGTGGACATACTAATGTTGTTTCCTATTTGCTGGATTTTCCAAATAACATTT
CGTCAGTCCCAGCTACAGATATGTCTCAATTGACTCCACCATCTCATGATCAACCTCCGATTCCACGTGTACCTATGAATACTTTGGCAATGGTGGTACC
CCCTCAAGAGCCTGATAAAGCTCCTGAACAATATGCAACAGAAAGAGCAAAATTAAAGCAAAAGCCAGATAACCTGCATGCATCTGATCAGGATCTGCGA
TCACCTTTTCATCCATGTCAGCTGTTGGAATGTATAGTAGAAGAAACCGAAGGAAAGCTTAATGAACTTGGTCAAAGAATTAGTGCTATTGAAAAGGCAC
AGCTCAAATCATTAGAACTAATTCAAGGTGAACCTCTGAATAAAGATAAAATTGAAGAGCTTAAAAAGAACAGAGAAGAACAAGTCCAGAAGAAAAAGAA
AATTTTAAAGGAGCTTCAAAAAGTAGAAAGGCAATTGCAAATGAAAACACAACAGCAGTTCACCAAAGAATATTTGGAGTCGAGCCCCCCTAAAGATCCA
ACAGTTACGCATCAGTGCCCTCTTAAAAGCGCAATCCCACCATTAGATTCAAATGATGATTTGTCAGAGGAGAACTGTGTGAAATTGTCTCAGGTTGGAA
ATATCTTGGCTGATGATGGTGATCATAAGTTATTGCCTCAGCCAGATGTTGAACAGATGGGATTTGTACCTATTCATCCATCAGCAACATCATATTCTGA
ATTATCCAATGAAATTGAATTCTGTGAAATGAATTCTCTTCAGCCACCAATATATGGAAACCAACCATTACTAGAACAGCAACAAGCGGCTAATCAGGTA
CTTTTATCTCAAGCACCAGATGCATTAATGGTTGCCTCTCCAGCACAGACCCTAACTGAAACCCTTGATGACATCATGGCTGCTGTGAGTAGTAGAGTGC
CTTCTATACCTGATGAATCCTCACTTCCCACTCCATCTTCGCTAAGTCTTCAGTCTACTCCAACGCCACAACCGTTGCTCTCTACTTTCCCCTCAATAGA
TATTGATGCGCATACTGAAAGTAATCATGATACCGCATTAACCCTTGGATGTGCTGGAGGTCACGAAGAGCTAGTGTCTGTGTTAATTACACGTTCTGCA
AATATTGAACACAGAGACAAAAAAGGTTTCACCCCCCTTATTTTGGCTGCTACTGCTGGACATGTTGGAGTGGTAGAGATTCTGTTGGATAAAGGAGCTG
ACATTGAAGCACAGTCAGAACGTACTAAGGATACTCCTCTCTCGCTTGCATGCTCTGGTGGGCGTCAAGAGGTGGTTGAATTGCTTTTGGCTCGGGGTGC
TAACAAAGAGCACAGAAATGTTTCAGACTATACCCCACTCAGTTTGGCTGCATCTGGTGGATATGTCAATATTATTAAAATCCTTCTTAATGCTGGTGCA
GAAATTAACTCAAGAACTGGCAGTAAACTTGGGATTTCCCCTTTGATGTTGGCTGCAATGAATGGACATGTCCCTGCAGTTAAACTGCTTCTTGATATGG
GTTCTGACATCAATGCTCAGATTGAAACAAATCGTAATACTGCACTTACTTTGGCATGCTTTCAAGGTAGAGCAGAAGTTGTGAGCCTTCTCTTAGATAG
AAAAGCCAACGTTGAACATAGAGCTAAGACTGGACTTACACCGCTAATGGAGGCAGCCTCAGGGGGTTATGCAGAAGTGGGAAGAGTTCTTCTAGACAAA
GGAGCTGATGTAAATGCACCTCCAGTGCCTTCATCAAGGGATACTGCTTTAACAATTGCTGCAGACAAAGGCCATTACAAATTCTGTGAACTGCTAATCA
GTAGAGGTTCACATATTGATGTTCGAAACAAAAAAGGAAACACACCACTTTGGCTGGCTGCAAATGGTGGTCATTTTGATGTAGTACAGCTTCTTGTGCA
GTCTGGGGCTGATGTTGACGCAGCTGATAATAGAAAGATCACTCCTCTTATGTCTGCATTTCGAAAGGGTCACTTAAAAGTGATACAGTTTTTGGTTAAA
GAAGTAAACCAATTTCCATCTGATATTGAGTGCATGCGCTACATTGCCACTATAACTGATAAGGACCTTTTGAAGAAATGCCATCAATGTATGGAAACAA
TTGTAAAAGCAAAAGACCAGCAGGCTGCAGAAGCAAACAAAAATGCAAGCATCCTCCTTAAAGAGCTAGATTTAGAGAAGTCAAGAGAAGAAAGCAGAAA
ACAAGCTCTTGCTGCAAAAAGAGAAAAGCGTAAGGAGAAAAAAAAAAAAAAA
5' EST: EL830361 [+]agtttacaagaagttaatggctaggaaacgggttgctttgcttatttttttaattacttcagatgtctgcttttgaggagttcgcgttttggaagtcggc
acagtaagcagacaaagtttaaagactcgtcgtttcgctcttcagatctcggtctttttccacgcttgacgggagctatgcagaatgctgtgaccgggac
ggacatgctcacggaaggctttgaagatgagatcgactcggtaactccgcgctcgccagtgacggagctgggaagtgaagccggtgtcggactctccgct
gccaaggttcgcctttttgggggcgcgtcgactggaaacgagagactggattttaggttggctgctgccgctgtgctctcctccggaactggctctggca
gtgacgaggacgaagtgtctgaagttgagtcgtttattttggaccaagaggacttggacaatccagtgcttaaaactgcatctgaactcttcctgtctac
tgctgctgaaggaacagactttagaactgttgatcctgagacacaggcacgtctagaagctttattagaagctgcaggaattggtaaattgtccactgct
gatggtaaagcctttgcagatcctgaagtacttagaagattgacatcgtctgtgagctgtgctttggatgaagctgcggctgcactaactcggatgagag
cagacaatactctcagcacaggacaagtagacaatcggagtctcgcagaagcctgttcagatggagatgttaatgctgttcgtaaactgcttgatgaaag
gtagaagtgtaaatgaacacacggag
3' EST: EL830362 [+]tttttttttttttttctccttacgcttttctctttttgcagcaagagcttgttttctgctttcttctcttgacttctctaaatctagctctttaaggagg
atgcttgcatttttgtttgcttctgcagcctgctggtcttttgcttttacaattgtttccatacattgatggcatttcttcaaaaggtccttatcagtta
tagtggcaatgtagcgcatgcactcaatatcagatggaaattggtttacttctttaaccaaaaactgtatcacttttaagtgaccctttcgaaatgcaga
cataagaggagtgatctttctattatcagctgcgtcaacatcagccccagactgcacaagaagctgtactacatcaaaatgaccaccatttgcagccagc
caaagtggtgtgtttccttttttgtttcgaacatcaatatgtgaacctctactgattagcagttcacagaatttgtaatggcctttgtctgcagcaattg
ttaaagcagtatcccttgatgaaggcactggaggtgcatttacatcagctcctttgtctagaagaactcttcccacttctgcataaccccctgaggctgc
ctccattagcggtgtaagtccagtcttagctctatgttcaacgttggcttttctatctaagagaaggctcacaacttctgctctaccttgaaagcatgcc
aaagtaagtgcagtattacgatttgtttcaatctgagcattgatgtcagaacccatatcaagaagcagtttaactgcagggacatgtccattcattgcag
ccaacatcaaaggggaaatc
Library:
Description: The library was made from dT primed cDNA and cloned into vector pCS107. PolyA RNA were primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3') and digested with NotI. cDNA was size selected using 1.1% agarose gel electrophoresis (>0.6kb) then ligated into NotI and SalI digested pCS107 vector. Primary library, non-amplified. Library constructed at the DOE Joint Genome Institute (Walnut Creek, CA) as part of the Xenopus Gene Collection project.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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