XB-CLONE-2506515
Clone Name: IMAGE:8917211
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Gene:
yeats2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC157664 [+]
CGTGCTGCGTCATTGCGCTCTACACTTGCAGCCGGGACGCCGCTATCTCGCGAGATGAGACTGGTGACGTGAGGACTGGAGGGCCGGCAGGAGCCGTACT
GTGGGGCATTTAAACAGCGGATCCGGGGCTGCGAGTTCTTTAAAGAAAACGGGGACTGTCCCGCCAAAAGCGGGAGTTGTTGGGCGTTGTGTGAAGTATG
TCTGGAATAAAGCGATCGTCCGAAGATAAAGATCCTGACTATGAAGACATTTCCGTTGTGCATCAGAATAAGCGGCATAAGGCTGCTGAAACTACTGCTC
GGGATGCTACCATCTTGAAGATAGAATCCATTATTAAAGAACAGTTCGTTACAGAGCTAAAGAATAAAGAACATGAGGTTGAAGTTATCGACCAGAGACT
GAATGAGGCCAGGCGAATGATGGATAAACTACGAGCATGTATAGTGGCTAATTACTATGCTTCTGCAGCACTTAACAAAAACCAGGAGGGACCCTCAAGT
TATGATGCAACTGTTTTAAACCATCCTTCTATTAAGAAGTTTTTGGAATCCCCATCTCGAGCATCATCACCTGCAAATCAGCGCTCAGAAACTCCATCCG
TGGCCAATTCAGAAACAGATTCTGTTTATACCCATAATGAGGATAAAGATGGCAGCCTTGATGCAAAAGGGCCAGCAGCAAACAAGGCACTTCGACCCGA
AGGGAATACCGTCAAGGATCCATCTATAAATGAAAACACAGAACGGCAGGTTTCAAGAACTGAAGACGGCCCTCGGCGATTTGTGAAAAAGACCATTGTG
GTTGGCAATGTTTCAAAGTATATTCCACCTGATAAGAGAGAAGAAAATGACCAGTCAACCCATAAATGGATGGTATATGTAAGAGGCTCTAGAAAAGAAC
CAAGCATTGATCATTTTGTAAAGAAGGTCTGGTTCTTTCTGCACCCAAGCTACAAACCAAATGACCTTGTGGAAGTCAGTGAGCCTCCATTCCACTTAAC
CCGAAGAGGCTGGGGAGAATTCCCTGTAAGGGTGCAGATTCATTTCAAGGACAGCCAGAACAAGAGGATCGATATAATCCATAACTTAAAGTTGGATCGA
ACGTACACCGGTCTGCAGACTCTGGGTGCCGAAACGGTTGTGGAAGTAGAAATCTACAGGCATTCTTTAGGCGAAGATTTCCTGGGCTCCCAGTCATTTA
ATGAATGCGCCCAATCAGAGGCATCCTTACTATCTCATGTAAAATCCTCCTCCCCTCACAAAGTAGCACATGAATCAGCAGATAAAGAGCCTTCATATTA
TCCTCAGCCATCATCACTTGAGCGCACCCCGACCAAAGTGGCACAGAGATTGTCCTTTGGGATTCCTGGCAGTACTGCTTTCCAGCCCATCACTGCCAGC
TGTAAGATTGTCCCACAGGGTCAGGCTGCCTCCTTAGCAGAGTCTCCTGGGAAGTCATTCCAGCCAATAACCATGAGCTGCAAGATTGTCTCAGGAAGCC
CCACAAGCAAACACTCCAGTACGTCTCAAGGTGCTTCTGGGACAAAGTCTCCTGTCCCAAAGCTCCATGGAACAAGTTTCACACCACAGAATATCAAGCA
GGAGGATTCTATGTTTGCTGCCATGCCTCCTCTGTGTCCTATTGGTAGTCAACCAAAGGCACAGAGCCCAAAGACGGTCACAGGAGGACTTGGAGCTTTT
ACAAAGGTGATTATAAAACAAGAGCCCACTGAACCAACACAGCAACTGCAAGAAGCCCCTTCCACGTCTCAGCCTCCACTTCAGCAGATTGTGACTGTGA
AAGGCAATCACATGGTTGCAGTGTCCCCACAGAAGAATTTGGCCACCTCTGAAGGTTCAAAGGTTGTTGGTATTCCAGTTGGAACTACAGTCCAGGCAGC
CATTAAACCATCTGTGACCATTAGCGGCGGGCAAATTCTAGTGGCTAAATCCAGCTCCTCCATAGCCAAAGCAGTGGGATCCAAGCAGATAGTTGCACAG
GGTGTGGCTAAAGCCGTTGTAAGCAGTGGAAGTGGGAATGTGGTAGCTCAATCTTCACAGAGCGTGTCAAAGGCACAGGTTACATCTGCTGTAGCTCAGA
AGGCTGGATCCCAAGGATCAGTGATGGCAACACTCCAACTCCCAGCTTCTAATATTCCAAATCTGGCCAATCTACCTCCTGGCACCAAACTGTACCTCAC
AACGAATAGCAAGAATCCATCAGGAAAAGGAAAACTGCTGCTTATTCCCCAAGGGGCCATTCTAAGGGCCGCAAACACTGCTAGTCTGCAGTCTGGAGCA
TCCTCAAGTGGTGGATCCAGTACTGCAAGCACCACCAGTGGGTTACCCCAGCACCTAACGTATACCTCCTATATTCTCAAGCAAACTCCCCAGGGGACAT
TTCTAGTTGGTCAGCAATCCCAGCAGGCATCTGGAAAGCAGGCTGCTGCAAATGTGATAGTTCAGGGGAATGTGGCCACGTCAGCTCAGGGCCACCAGAC
CTTGAAATTAACCACTGGACCGAAAGGCACTTTAGTTGCACAGACCCACAGTGCATCAGCCGCAGTTGCAAAAATTTCAGACGGTGCAGTAACATCGGTA
CAATCTCAGACCCAGAAGCCTGGTACCACTTTGCTAAGAGTGGCAGGCGGAGTCATAACAGCCTCCAGTGCATCCTTACCCTCTGTTTCCACTAATGTTT
CTACACAACAGGCAGCAGACGTGTCCAGCACATCCACAACCCAAGTGAGCGCATCAAACAAGTCTATATCCCAGCAGGTTATGGCTAGGATTGCTCATGT
CAAGTCACTGGGCACAGTGAAACCTGCCACTTCAGCTGCTGCGGTGTCTTCAACAAGAACAGCACCGACTCTAGCACAAAGCAGTGCTGCTTCAGCAGTA
AAAATGGAACCAGATATGACTTCAAATGCCAGCAGCACATCAGGGCCAGGTTCCAGCACACCAGTAAAGACTGAAGAGAGTGGAGAAAGTGACCCCAGTT
CAGTGAGGGTAGTTGGTCAGGTTGAGTGTGCAAGCCTCAGGGCACGACATAACTGGACCGAACATCTGCAGAACTTCAAGCAACTTTTAACTGCAATTGT
GAAGAAGGTCCCTCTTCTAGCTGAGAAAAGCGAAGATGGTAACGCATTTTGTGCAACATCGGTTGAACAGTATTACAGCTGGAACATTGGAAAGAGAAGA
GCTTCCGAGTGGCAACGAGCAGTTACAATGAAAAAAATGCTGCAGGAGATCATCGAAAAGCAACCAAAGTTCAGTAATGTGGCGCCGCTCAAAACCAAAC
AGATCGTCCACTGGTGCCGCATACATGGATACACACCGCCTGATCCTGAGAGCCTGGGCAGTGATAAGGAATCCATTGAAGATGTGCTGGCTCGTATAGA
TAATGAGGCGGCTTTACAGACATCATTCTCTGCTCCTGACATTCTCTGCCAAAAAATGGAGGAACTAGAGAAAACGTTTAAAAAGGAAACAGAAGAGGAT
GTAGATGTGGATGTGCTGAGTGCCAATGAAGAACAGAGATTGCACATTAAGAAAGAGAGTGAGGCAGAGGAAGATGCAAAGCTGTACATGGTAACCAGTC
CTGCTTGTGAGTTCATCAGAGATAAAGCACAGGAGATTGGTGTTTCAGTACAACCAGTCGAAGTGCAAGACAATATTTATACATCTGCTGTCGAAGAAAT
GATTTTAAAGGCTACAGAACAGTTTGTTAGTGACGTCCTTCGGGACGCTTTAGGAGTAGCCTATCAGATGACATCCCACAATAGAGCGCCACAAGAGATC
ACAGTATCAAATATCCACCAAGCCATCTGTAATATTCCTATGTTTGACTTCCTCACTAACAAACACATGGCCTCATCAACTACGGAGGATGAGCAGTCCT
TAAAATAAGGTGTGAAAGTGGCACAAGGAAAACTACTGACAGAGCCATCTGTTTGCCACCTAGTGGATGGAGGATTCCGAGGCTTGGACATTCTCACTGA
GAAATGAAATGCAACGTCTTACAGCTAGAACCTACGCGAATTGTTTTCTTATGTAAATAATGTCGGTGTAACTGTGTGTGAGAGAACCTGTAACTATGTA
TTTGTTATGTGTACATGCAGATCTGTATTTATGTACATGAGTTTTTGTATTTCCCTGCATGCAATAATGCAAGGTGCAAGCTAGGCCAAAGTGAATAAAA
CACAAATCCCTCTAGTTAACCTACAAAAAAAAAAAAAAA
5' EST: EL830378 [+]cgtgctgcgtcattgcgctctacacttgcagccgggacgccgctatctcgcgagatgagactggtgacgtgaggactggagggccggcaggagccgtact
gtggggcatttaaacagcggatccggggctgcgagttctttaaagaaaacggggactgtcccgccaaaagcgggagttgttgggcgttgtgtgaagtatg
tctggaataaagcgatcgtccgaagataaagatcctgactatgaagacatttccgttgtgcatcagaataagcggcataaggctgctgaaactactgctc
gggatgctaccatcttgaagatagaatccattattaaagaacagttcgttacagagctaaagaataaagaacatgaggttgaagttatcgaccagagact
gaatgaggccaggcgaatgatggataaactacgagcatgtatagtggctaattactatgcttctgcagcacttaacaaaaaccaggagggaccctcaagt
tatgatgcaactgttttaaaccatccttctattaagaagtttttggaatccccatctcgagcatcatcacctgcaaatcagcgctcagaaactccatccg
tggccaattcagaaacagattctgtttatacccataatgaggataaagatggcagccttgatgcaaaagggccagcagcaaacaaggcacttcgacccga
agggaataccgtcaaggatccatctataaatgaaaacacagaacggcaggtttcaagaactgaagacggccctcggcgatttgtgaaaaagaccattgtg
gttggcaatgtt
3' EST: EL830379 [+]tttttttttttttttgtaggttaactagagggatttgtgttttattcactttggcctagcttgcaccttgcattattgcatgcagggaaatacaaaaact
catgtacataaatacagatctgcatgtacacataacaaatacatagttacaggttctctcacacacagttacaccgacattatttacataagaaaacaat
tcgcgtaggttctagctgtaagacgttgcatttcatttctcagtgagaatgtccaagcctcggaatcctccatccactaggtggcaaacagatggctctg
tcagtagttttccttgtgccactttcacaccttattttaaggactgctcatcctccgtagttgatgaggccatgtgtttgttagtgaggaagtcaaacat
aggaatattacagatggcttggtggatatttgatactgtgatctcttgtggcgctctattgtgggatgtcatctgataggctactcctaaagcgtcccga
aggacgtcactaacaaactgttctgtagcctttaaaatcatttcttcgacagcagatgtataaatattgtcttgcacttcgactggttgtactgaaacac
caatctcctgtgctttatctctgatgaactcacaagcaggactggttaccatgtacagctttgcatcttcctctgcctcactctctttcttaatgtgcaa
tctctgttcttcattggcactcagcacatccacatctacatcctcttctgtttcctttttaaacgttttctctagttcctccattttttggcagagaatg
tca
Library:
Description: The library was made from dT primed cDNA and cloned into vector pCS107. PolyA RNA were primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3') and digested with NotI. cDNA was size selected using 1.1% agarose gel electrophoresis (>0.6kb) then ligated into NotI and SalI digested pCS107 vector. Primary library, non-amplified. Library constructed at the DOE Joint Genome Institute (Walnut Creek, CA) as part of the Xenopus Gene Collection project.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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