XB-CLONE-2506116
Clone Name: IMAGE:8916970
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Gene:
drosha
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC157520 [+]
GCGAGATTGTGACAAAGGTTTAGGAAAGCGGGCCGGCAAAAGACAATGTCTCTGCATTCAGGAAGAGGTGGACCACAAGCCCGAGGAGGTCCTGGAGCCC
CCTTGCAGCCTTTTAGACCACAGAACCTAAGGCCTGCTCAGCCTCAGTCTTCAGGCCAGTACTGCTATGATCCACAAAGTACTCCACCTCCTCCGACATT
CTCTAACATGCCAAACACAAATTACGTACCACCAAGACCAGATTATATGTCATATCCTCCGCCTGCTACTCCTGCAACGCAGAGTGCAATAAACCAATGT
CTACCCAGGCCCCCTTTTCCAGTTAGGCAGAGTTATCCAATGCCTCCCTGTTTTCCTCCAGCTCCACCTGCATCTGCCCCAGGCAATCCACCAGCTGTAG
TAACTCCTGTTGGACAGTGTCCGTATCCTTATATGATGCCACCAGTTATGCCACCCCCACCAATTATGCCTCCCCCATTACCCCATGTGCCCTATCAACC
TCCATATTCTGCTGGATACCCTCCTCAACCTTTTCCTCCACCACCTCCGTCACCACAACCAACACCAAGCTTTAACAGCTATCAGCAGGCGGCACCATCA
TTTCAAGGTAACAACCACTACAGACATGCAAGTCATGGGCAGTACCAGACTGAGAAGTCACAAACTGATCACAGAGTGCAGGAAAGAGGCAAACACTATG
AAGATCATCGCCGCAGAGAGTACAGCCATAGTGACAGACACCGGTCTGAAAAGCATAGCGATCGGCGAGAGCGAGGCCGAAGCTCTGAAAGGTCACGAAG
ACTAGATGGCAGCCGTCACAGGTCAGATTATGAGTATAGAAGGGGACCATCACGTGGCAGAACTCCATCACGCCATCCAAGTTACGAAAGAAGCAGCCGA
GAGAGACACCGGCATAGAGAAAGCCGAAGATCAACATCTACTGAGAGAAGTTATAGAAAGAGGCCAAAAAGTCCATCACCATCAAGCAGAGAGCATAAAA
GATCTCGTTGGGAGGAAGACAATGAATCTCTATCAGAGAGCTCTTCAGCAAAAGAAAAGTCCAGCATTTCTTTTAAAGAGAAGGATTCTCTAGATGAAAA
CCTATTGGAAAGAAATGAGGAAGAAGATGAAGAATTGCTTAAACCAGTGTGGGTTCGTTGCACCCATTCTGAGAGTTACTACACTAGTGACCCCATGGAT
CAAGTTGGAGACTCCACAGTGGTTGGCACAAGCAGACTACGTGATTTATATGACAAGTTTGAAGAGGAGCTTGGAAAGCGTCAGGCAACAGCTAGAGCTG
CCAGGCCAGCATGGGAGCCGCCAAAGACCAAATTAGATGAGGATCTAGAGAGTTCCAGTGACTCGGAGTGTGAAACGGATGATAGTTGCTCCAGCAGTTC
AGATTCAGAGGTGTTTGATGTAATCGCAGAAATAAAAAGGAAAAAGGCACACCCCGATCGTCTTCATGAAGAGCTCTGGTATAATGACCCTGGGCAGATG
AATGATGGACCTCTCTGTAAGTGCAGTTTGAAAGCCAGACGAACTGGGATCAGGCATAGCATTTACCCTGGAGAGGAGCCTATCAAAGCATGCCGCCCCA
TGACAAACAATGCAGGACGGCTTTTCCACTACAGAATAACAGTGTCTCCACCAACTAATTTCTTGACTGACCGGCCAACTGTTATTGAGTATGATGATCA
TGAGTATATCTTTGAAGGTTTTTCCATGTTTTCTCATTCTCCACTGACAAATTTTCCCCTGTGCAAAGTAATCCGGTTTAACATTGACTACACAATTCAT
TTCATTGAGGAAATGATGCCTGAGCATTTTTGTGTGAAGGGCTTGGAACTATTTTCTTCCCATTTGTACAAGGATATTTTGGAACTTCATGACTGGAATC
TTATGGATCCTTCTGTTGAAAACAATGATGCCAATTGCCCACAGTTTCATTTTATGCCCCGTTTTGTACGATTTCTGCCAGATGGTGGTAAAGAGGTCCT
CTCCATGCATCAAGTTTTATTGTACTTGTTGCGATGTAACAAATCTTTAGTTCCTGAAGAAGAGATTGCAAACATGCTTCAGTGGGAAGAGCTTGAATGG
CAGAAATATGCAGAAGAGTGCAAAGGGATGATTGTCACTAATCCTGGCATGAAACCCAGCTCAGTGCGAATTGATCAGCTTGATCGGGAGCAGTTTAATC
CAGACGTTATTACATTCCCTATAATTGTCCATTTTGGAATAAGGCCTGCTCAACTGAGTTATGCCGGAGACCCTCAGTACCAGAAATTATGGAAAAGCTA
TGTGAAACTGCGCCATCTGCTGGCTAATAGCCCGAAAGTCAAACAAGCTGACAAGCAGAAATTGGTCCAGAGGGAGGAAGCTCTTCAGAAGATTAGACAA
AAGAACACAATGCGACGAGAAGTAACTGTAGAGCTGAGTAGTCAAGGATTTTGGAAAACTGGTATCCGCTCCGATGTCTGTCAGCATGCAATGATGCTCC
CGGTTCTGACCCATCACATACGCTACCATAAATGCTTAATGCATTTAGATAAATTAATTGGATATATCTTTACAGACCGCTGTTTGCTGCAGCTTGCAAT
GACTCATCCAAGCCACCATTTAAACTTTGGAATGAACCCCGACCATGCTCGAAACTCCCTCTCGAATTGTGGTATCAGGCAGCCAAAATATGGAGACAGA
AAAGTGCATTACATGCATATGCGTAAAAAGGGAATTAATACACTAATAAATATAATGTCACGTCTAGGACAAGATGATCCTACCCCTTCCAGGATAAACC
ACAACGAGCGTCTTGAGTTCTTAGGAGATGCGGTTGTTGAATTTTTAACAAGCGTCCACCTATATTACCTGTTTCCATTGCTGGAAGAAGGAGGATTAGC
TACCTACCGGACAGCTATTGTTCAGAATCAACATCTTGCCATGCTGGCAAAGAAATTGGAATTGGATCGCTTTATGCTATATGCGCATGGGCCTGATCTG
TGCAGAGAATCAGACTTACGCCATGCCATGGCAAACTGCTTTGAAGCACTCATGGGTGCAGTTTATTTGGAAGGTGGACTTGAAGAAGCAAAGCAATTAT
TTGGAAGACTGCTCTTCGGTAATCAGGACCTGCGAGACGTGTGGCTTAATTATCCACTACACCCGCTTCAACTCCAGGAGTCTAATTCTGATCGGCATCT
GATTGAGACATCCCCAGTTCTAAAGAAGCTCACCAAGTTTGAAGAGGCAATTGGAGTTGAATTTACACATGTTCGACTACTTGCTCGGGTTTTTACCTTA
AGAACTGTAGGATTTAACCATCTGACCTTAGGCCACAATCAGAGAATGGAATTCCTGGGTGATTCCATAATGCAGCTGGTAGCCACAGAATATTTATTCA
TACATTTCCCTGATCATCATGAAGGCCACTTAACGCTGCTACGTAGCTCCTTGGTGAACAACAGGACTCAAGCAAAAGTAGCAGAGGAACTTGGCATGCA
AGAGTTTGCCATCACAAATGACAAGACTAAGAGACCAGTGGTGCTCCGCACAAAGACTTTGGCTGATTTATTAGAATCCTTTATCGCTGCCCTTTATATT
GATAAGGACCTGGAATATGTCCACACGTTTATGAATGTTTGCTTCTTTCCACGACTGAAGGAGTTCATATTGAATCAAGACTGGAATGATCCAAAGTCTC
AACTTCAGCAGTGCTGCCTCACTCTAAGAACAGAAGGCAAAGAGCCAGATATCCCTCTGTACAAAACACTACAAACAGTAGGACCCTCCCATGCTCGAAC
GTACACAGTTGCTGTATATTTCAAAGGAGAAAGAATTGGCTGTGGTAAAGGCCCCAGTATTCAGCAAGCTGAAATGGGTGCAGCAATGGATGCGCTGGAG
AAATATAACTTCCCCCAGATGGCTCATCAGAAGCGCTTTATCGAACGGAAGTATAGGCAGGAATTGATAGAACTCAGGTTGGAGAAGGAGCAGCAGGAAT
TAGCAGAAGAGATGAGAAAATAAAGCCTGGCGCTCTTATAGTATCCATCCAACTCACTGGGGACAGCTGCCATGCAATCCGGTCCTTCGTGTGGGTTTAG
ATACATGTCCAGCTGTGTCTGGGGAAACACAAGATAAATCATGCATCTTTTTTTTGTTTTGTTTTAGGTGTCTTTTTTTTTTTTTAACTTGTCACATTTT
AGTGGATTTTATCCATAGCTGAGCTATTTTCTTTTTATTTAAATACTTTAGTAAATTATTGTTTTTATTATATTGTTTTTATAAAATAAATGGCAGGTGT
GTAATAAAAAAAAAAAAAAA
5' EST: EL830638 [+]gcgagattgtgacaaaggtttaggaaagcgggccggcaaaagacaatgtctctgcattcaggaagaggtggaccacaagcccgaggaggtcctggagccc
ccttgcagccttttagaccacagaacctaaggcctgctcagcctcagtcttcaggccagtactgctatgatccacaaagtactccacctcctccgacatt
ctctaacatgccaaacacaaattacgtaccaccaagaccagattatatgtcatatcctccgcctgctactcctgcaacgcagagtgcaataaaccaatgt
ctacccaggcccccttttccagttaggcagagttatccaatgcctccctgttttcctccagctccacctgcatctgccccaggcaatccaccagctgtag
taactcctgttggacagtgtccgtatccttatatgatgccaccagttatgccacccccaccaattatgcctcccccattaccccatgtgccctatcaacc
tccatattctgctggataccctcctcaaccttttcctccaccacctccgtcaccacaaccaacaccaagctttaacagctatcagcaggcggcaccatca
tttcaaggtaacaaccactacagacatgcaagtcatgggcagtaccagactgagaagtcacaaactgatcacagagtgcaggaaagaggcaaacactatg
aagatcatcgccgcagagagtacagccatagtgacagacaccggtctgaaaagcatagcgatcggcgagagcgaggccgaagctctgaaaggtcacgaag
actag
3' EST: EL830639 [+]tttttttttttttttattacacacctgccatttattttataaaaacaatataataaaaacaataatttactaaagtatttaaataaaaagaaaatagctc
agctatggataaaatccactaaaatgtgacaagttaaaaaaaaaaaaagacacctaaaacaaaacaaaaaaaagatgcatgatttatcttgtgtttcccc
agacacagctggacatgtatctaaacccacacgaaggaccggattgcatggcagctgtccccagtgagttggatggatactataagagcgccaggcttta
ttttctcatctcttctgctaattcctgctgctccttctccaacctgagttctatcaattcctgcctatacttccgttcgataaagcgcttctgatgagcc
atctgggggaagttatatttctccagcgcatccattgctgcacccatttcagcttgctgaatactggggcctttaccacagccaattctttctcctttga
aatatacagcaactgtgtacgttcgagcatgggagggtcctactgtttgtagtgttttgtacagagggatatctggctctttgccttctgttcttagagt
gaggcagcactgctgaagttgagactttggatcattccagtcttgattcaatatgaactccttcagtcgtggaaagaagcaaacattcataaacgtgtgg
acatattccaggtccttatcaatataaagggcagcgataaaggattctaataaatcagccaaagtctttgtgcggagcaccactggtctcttagtcttgt
cat
Library:
Description: The library was made from dT primed cDNA and cloned into vector pCS107. PolyA RNA were primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3') and digested with NotI. cDNA was size selected using 1.1% agarose gel electrophoresis (>0.6kb) then ligated into NotI and SalI digested pCS107 vector. Primary library, non-amplified. Library constructed at the DOE Joint Genome Institute (Walnut Creek, CA) as part of the Xenopus Gene Collection project.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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