XB-CLONE-2501617
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Clone Name: IMAGE:8919482
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Gene:
nup155
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Species: Xenopus tropicalis
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Sequence:
5' EST: EL833537 
[+]
tacattttgagcaaatgctgaagttggcacagcgttctacagatgagctcttcaacatagcacttttcaactggcttatacaggcggatctcactgacaa
actgctggagttgaattctccgtttctggagccacatttggtgcgtatggcgaagctggatcagaacaaagtgcgatacatggatttgctgtggcgatac
tatgagaagaacagaaatttcagcaatgctgctcgtgtcgtggccaaacttgcagatatgcccagcacagagatctcattaaagcaacgacttgaataca
tctccagagccattctcagtgccaagagctccactactatgtccacccttgctgccgacggcgagttcttgcacgagctggaagaaaagttagaggtagc
aagaattcagctgcaaatacaagagaccctaaccagacaatattcccaccattcggctgtgggggatgcagtgtcgcagcttgattctcagctaatggac
attacaaagctgtttggacaatatgctgatccatttaggctttcagaatgcaaactagcaattatacactgtgctggacattcagatcctatattggtgc
agacactctggcaagaaatcattgataaagaattgagcgacagcctgggaaatagctcggtagatcgaatgcagtcacttcatctaaaaatgacttctct
tggaaaaatctatgccagtacccctcgctattttcctttggaatttttggtgaaatacctgggaacaaca
3' EST: EL833538 [+]tttttttttttttttgttttgtaaaattaatttataatctttttcaaaaccctgtttggcagtcagaatatttaaggaagaaaatatgaattataacaaa
gtctcctcatttaatgcaggaggaaaaatgtcgccgtgtgtgggaaacagaccagaatgcagtgtggcgatgggtcaggcagtaatcgaggaataaaaca
ttcgtattgagagatctaaatcaggacacacaaaggtaaatgagtggaaccatgccgaaagcaatggcagccttcatggtatccacaacccgtccaaatg
tgtgtacagctgtccattatgttgaatcatgaatctgactggcttgactagctcagccgctctaatttagcttgcaaagatttgaagttagatacagtat
tcagcaacgccggagccggatccatcgactgcagctccactaggtagcaggatattgcatccaggcagagtgtggtaaaccggcgcctttcataggaagg
cactttgcttggctcctgtacatacccagagagcaagagataaatgctttctagcaagtgcaggggcttcttcatcctactccaccagggatcccttgct
ttaaacaagtgatcatatacttcaagtaacttgggaacaggcacattgatctcttgcattgtgtacgttacaaagccagcgtcccagctgaagttacaca
cctgttgttccaggtatttcaccaaaaattccaaaggaaaatagcgaggggtactggcatagatt
Library:
Description: The library was made from dT primed cDNA and cloned into vector pCS107. PolyA RNA were primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3') and digested with NotI. cDNA was size selected using 1.1% agarose gel electrophoresis (>0.6kb) then ligated into NotI and SalI digested pCS107 vector. Primary library, non-amplified. Library constructed at the DOE Joint Genome Institute (Walnut Creek, CA) as part of the Xenopus Gene Collection project.| Anatomy: testis | Stage: frog |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
Usage:
Suppliers:
