XB-CLONE-2495092
Sequence:
Full length sequence: BC155394 [+]
CAAACAACATGGACACTGACTTCACAGGCAAAGATGTTTATGAGAAGGAATTTGATCCTTGGAGTTATTTAGATACATATTATAACCCAATATCAGGCCT
TGTGACGAAGGATGGTAATCTGGATTTTGCGTTAAAAAAACTTCATGAGGCATTTACCATGCATGGAGTGAAGGGGGAAACACTTATTGACGTTGGCCAT
GGACCAACAATTTATCAGGAACTTTCTGCATGCGAATCCTTCAGAGAGATCATTGGTGCTGAATACACTGACCGTAACCGCGAGTACTATGAAAAAACCC
TGAGAAATGAACCAGGAATATTTGACTGGACAAGTGTTATACAAGCAGTCTGTGACCTGGAGGGAAAGGGTACAACAGTGGAAGAGAAGAGAGAAAAACT
GAAAAAGACAGTGAAAAAGACTATCAAATGTGATGTAACTAAAAGCAATCCTGTGGCACCGCTGGTGTTGCCCAAAGCTGACTGTGTAATGGCTATTGGG
TGTCTGGAATCTGCCTGTGAAGATCTGGATAGTTATTGTAATGCCCTTAATAATATTTCATCCTTGCTAAAAGTGGGGGGGTATTTAATAATCTCCGAAG
TCCTTAACTGTACCACTTACTTGTTTGGAGACAAAAGGTTTTCTTGCTTATCGTTAAATGAGGAATTTATGAGAAGTGCTATAACCAATACCGGTTATGC
TATAATAGACCTGGAGGTTTTTCCCAGAAAGTATGATGAAGAGCAGTACAATCTCTGTGATTATGACTCTAATATATTTGTTCTTGCACGCAAACTGAGG
GATATTTAGGCTGGAGAGGACAGTACATAGTATATGACTGCTTATGTCTATTCTCTACCAAGATTTCTTTACACCTTCAGTTATAAAAAATATACCTATT
CTGAACTAAAAAAAGAATCATTACTGCTCTATTTAAATTTCTCTATGGGGCTGATTCAAGAATGCTCAAAGCTTGCACATTCTTAAATTGGTCCCTTTGC
CATTGTCACCTAAAGGAGTAAACATTTTTTTCTGCCCCTAAATAGCAATAGATCTATAAATTTAAATGGTTACACCCTTTTTAGGAGGAACAGAGGGAAT
GGAAAAGGTGGAGGGGCTTGTCTGTATATAAAGCCAGAATTAAAGCCTTGCACTAAAGATATTAACAGGGACAGCATGTACTCTTTATGTTTCCTATAAA
TTAAAATTCAAAACACTTTATGGACCAATACTATTATTGGACATTCTACAATAGTGACAAAAGAAGGTTCCTAACTTATTTAAACTCTAGACTTGTCTCA
TTACAATAATCATATATGGTATAGACACAGTGCCATCTATGTAGAAAACCCCCCAGATGTACTAAGATATTTGAAAAAAAAGCTAAAAATGTCTACAGCC
CTGCCAAGATTTGAAGGGGTCAAATCAGACAGGTAAGGTCCGACCTGAAATTCAAACCATAAGGCAATACACAATTCTATGATAAGTGTTTTTCACTTAT
TTAATACCTGTTCAATAACTGTGATCCTCATAACATCCAGTAAACAGGGCTGTCTCAACAAAATATGGGGAATGTGAAACTCAGAACTTATATGCTTCAA
ATTTCAGCAGGGGCTAAAGGTTACAAAGAAAATTATCATTGATGTATGCTATAAACCACCCTGTATAAGTGAGGAGGATGAGGCCCAGCTACTGTTACAA
ATGAAGGAGGCCTCACAACTAGGTCAAGTTGTTATTATGTGGGACTTCAATTATCCGGAGATTGACTGGAGTAATGGGGTGGCTAAGTCAGAAAAAGCTA
GTAGGTTGGAAAAAGGCTAAATGACAACTTTTTATTTCAGGCAGTTTAAGAACCTACTGGAAATGATTCTCTTTTGGACCTGGTAATAACTAATAATGCT
GAACTTATCTCTAGCATTTGCATGGGTGAGCATTTAAGGAACAGTGATCACAACATGGTCTCCTTTGAGGCAACTCTATAAGGGAGTAACTAAAACACTA
AATTTTAAAAGTGCAAAGTTTGCCAGTATAAGGGCATCTCTGAAATATATTAACTGGGAAAGGCTGTTCCAAGGTTTAAACACAGAAGGAACATGGGATA
TCTTTAAAATGCTACTTAACAAGTATACATGTCAGTATATTCCCCTTCTAAGCAAGGAAATACATCACAAAGCAAAACCTTTATGGTAAATGTGCTAGTG
TGGAACTTAGCTGGAATAGCTGAAACTTTCATCACGTACAAGGAAGCAAATAAAGCATGCAAAAAAGCTATCAGGCAAGCTCAAATACAAATAGAAATAA
AAAAAAAAAAAAA
5' EST: EL837746 [+]caaacaacatggacactgacttcacaggcaaagatgtttatgagaaggaatttgatccttggagttatttagatacatattataacccaatatcaggcct
tgtgacgaaggatggtaatctggattttgcgttaaaaaaacttcatgaggcatttaccatgcatggagtgaagggggaaacacttattgacgttggccat
ggaccaacaatttatcaggaactttctgcatgcgaatccttcagagagatcattggtgctgaatacactgaccgtaaccgcgagtactatgaaaaaaccc
tgagaaatgaaccaggaatatttgactggacaagtgttatacaagcagtctgtgacctggagggaaagggtacaacagtggaagagaagagagaaaaact
gaaaaagacagtgaaaaagactatcaaatgtgatgtaactaaaagcaatcctgtggcaccgctggtgttgcccaaagctgactgtgtaatggctattggg
tgtctggaatctgcctgtgaagatctggatagttattgtaatgcccttaataatatttcatccttgctaaaagtgggggggtatttaataatctccgaag
tccttaactgtaccacttacttgtttggagacaa
3' EST: EL837747 [+]tttttttttttttttatttctatttgtatttgagcttgcctgatagcttttttgcatgctttatttgcttccttgtacgtgatgaaagtttcagctattc
cagctaagttccacactagcacatttaccataaaggttttgctttgtgatgtatttccttgcttagaaggggaatatactgacatgtatacttgttaagt
agcattttaaagatatcccatgttccttctgtgtttaaaccttggaacagcctttcccagttaatatatttcagagatgcccttatactggcaaactttg
cacttttaaaatttagtgttttagttactcccttatagagttgcctcaaaggagaccatgttgtgatcactgttccttaaatgctcacccatgcaaatgc
tagagataagttcagcattattagttattaccaggtccaaaagagaatcatttccagtaggttcttaaactgcctgaaataaaaagttgtcatttagcct
ttttccaacctactagctttttctgacttagccaccccattactccagtcaatctccggataattgaagtcccacataataacaacttgacctagttgtg
aggcctccttcatttgtaacagtagctgggcctcatcctcctcacttatacagggtggtttatagcatacatcaat
Library:
Description: The library was made from dT primed cDNA and cloned into vector pCS107. PolyA RNA were primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3') and digested with NotI. cDNA was size selected using 1.1% agarose gel electrophoresis (>0.6kb) then ligated into NotI and SalI digested pCS107 vector. Primary library, non-amplified. Library constructed at the DOE Joint Genome Institute (Walnut Creek, CA) as part of the Xenopus Gene Collection project.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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