XB-CLONE-2494703
Clone Name: IMAGE:8914640
|
Gene:
tamalin
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC160992 [+]
gcgagtcgtgtgaggagcgtgtgtatatggtgggagcgcaatgactttcaggcgcctgaggaagctgcagcacaaggaggaatatgcatgtcccccagac
cgaacggtgtacagagaccgggacaagctggatccttacagcgccctcaccagctacaagcaggctgacatgtacaaggctctggcggtgtccgggggaa
ccctgcccaggactaagaagggctctacagcaggatggaagaatgtcagccagtcatcagagtaccaaagaaaaatacttactttgcaaaaagaagactc
tgaatcctttggatttgaaattcagacatatggactgcaccaccaagacaagaatgcagtggaaatgtttacctttgtatgccgggtgcaggatggaagt
cccgctcagctgtgtggtctcaaagttggtgatataattgctggagtaaatggcctcaacatggatggagtcaggcacagagatattgtagagctcatca
aggtttctggaaacacaataaggctggagactgtgtatggatctgcaatcagaagggctgagctggaggctcgaatacaatatttaaagcaaaccttgta
tgagaagtgggaagaataccgctcgctcatggtgcaagagcagagactgctgcacggtatagtagtgaaagacccaagtgtgtatgacacccttgaatct
gtgagatcttacatctatggaacagtcaacacatgcagcaaagatcctctcactgggactttggccgctggaagtatgtgtagcagtgcaagttgcctca
gcactactgaggaaaatgaggatgcagtatatcagacttgctacttcagctcggattccacagatgaaataaacagccttccaggcaaaagtaacaacaa
tggaaagccttataagtcaacattgacacgaagctccagtgtaaagtgcccgggtcctgctacaaactgggacaaaccaaaggaacaaaataactttgtc
tctctgcccaggaaaaagcacaaaagttttcgaaaaagactcctaaaatttattcctggacttaaccgaaatttagaagaggaggccagccctctttaat
gtcccccctttctttatataagaagttaagttatttatttattgttctatttctggagaaaagtgaaagctttgtgttgaatttttttgttaaacttaac
tgtacaactttatagcttcggcatttgtttttcattaaaatgtttggcaaagcgagaggtgccaaactccaggtttttctatgaaaaaaaaaaaaaaa
5' EST: EL838001 [+]gcgagtcgtgtgaggagcgtgtgtatatggtgggagcgcaatgactttcaggcgcctgaggaagctgcagcacaaggaggaatatgcatgtcccccagac
cgaacggtgtacagagaccgggacaagctggatccttacagcgccctcaccagctacaagcaggctgacatgtacaaggctctggcggtgtccgggggaa
ccctgcccaggactaagaagggctctacagcaggatggaagaatgtcagccagtcatcagagtaccaaagaaaaatacttactttgcaaaaagaagactc
tgaatcctttggatttgaaattcagacatatggactgcaccaccaagacaagaatgcagtggaaatgtttacctttgtatgccgggtgcaggatggaagt
cccgctcagctgtgtggtctcaaagttggtgatataattgctggagtaaatggcctcaacatggatggagtcaggcacagagatattgtagagctcatca
aggtttctggaaacacaataaggctggagactgtgtatggatctgcaatcagaagggctgagctggaggctcgaatacaatatttaaagcaaaccttgta
tgagaagtgggaagaataccgctcgctcatggtgcaagag
3' EST: EL838002 [+]gctttgccaaacattttaatgaaaaacaaatgccgaagctataaagttgtacagttaagtttaacaaaaaaattcaacacaaagctttcacttttctcca
gaaatagaacaataaataaataacttaacttcttatataaagaaaggggggacattaaagagggctggcctcctcttctaaatttcggttaagtccagga
ataaattttaggagtctttttcgaaaacttttgtgctttttcctgggcagagagacaaagttattttgttcctttggtttgtcccagtttgtagcaggac
ccgggcactttacactggagcttcgtgtcaatgttgacttataaggctttccattgttgttacttttgcctggaaggctgtttatttcatctgtggaatc
cgagctgaagtagcaagtctgatatactgcatcctcattttcctcagtagtgctgaggcaacttgcactgctacacatacttccagcggccaaagtccca
gtgagaggatctttgctgcatgtgttgactgttccatagatgtaagatctcacagattcaagggtgtcatacacacttgggtctttcactactataccgt
gcagcagtctctgctcttgcaccatgagcgagcggtattcttcccacttctcatacaaggtttgctttaaatattgtattcgagcctccagctcagccct
tctg
Library:
Description: The library was made from dT primed cDNA and cloned into vector pCS107. PolyA RNA were primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3') and digested with NotI. cDNA was size selected using 1.1% agarose gel electrophoresis (>0.6kb) then ligated into NotI and SalI digested pCS107 vector. Primary library, non-amplified. Library constructed at the DOE Joint Genome Institute (Walnut Creek, CA) as part of the Xenopus Gene Collection project.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: