XB-CLONE-2494435
Clone Name: IMAGE:8914716
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Gene:
smc1b
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC160994 [+]
gtgattatatccccggcgtcggtgtatatatagggggggctttttatatagcctgttgggtagaaaggagtggctaccgggcggctgggaggggcctcgt
acctaccttactaggcctgagggaggagttagtgactgagtgatattggggggtctcagggggcaacatggggttcatgaagcagctgattattgagaat
tttaagtcctgggggggccggcaggtcattgggcccttcctgcgattcaactgtgtcatcggccccaatggctcaggtaaatccaatctaatggacgcct
tcagcttcgtaatgggagaaaagcccgctaacctgcgagtgagaaacatccggcagcttattcacggggccaacgttggcaagcccatgtccacgacggc
aagggtagtcttggtgtacagtgaggaaaatggagaggagaggcagttttctaggattatcgtaggcgactcttcagagtatcggattgatggcaagcca
gtagggcgttccacatatgtaatggaactggagagaattggcatcattgttaaagccaggaactgcctagtatttcagggagaagtggaatctcttgcta
tgaagaagcccagagagaggactcatctgtttgagcaaataagcaactcctgggagctagcagatgaatatgagaaaaagaagaagctgatgctgcaggc
agaagaggatgcgcagttttgctataataaaaagaaaaatgcagccgttcaacggaagcatgccacgttagagaaggctgaggctgatcgctacaaagca
ctgcaacagaatctgaaagaaaccaaggtcgagctacaactatatcagcttttccacaacgagaggaaactggagtcggtagtgaggagtttagaagaga
aacaatcagatgccaataggcagaaagagcagcttgtccgtgccgaatcggctctcaagtctgaaaaagcagagctcggtcgatgtacgagagatctgca
gcaaattgaaaaagagattaaagttcaagaagtgtctctaagtcatttacgaccccagtttatcaaagccaaagaaaacaccgctcatcatttaaagaaa
gtcgaaacggctaaaaaaaacgtagcaaacaatgagaagcagtgccacaaattggaacaagacaagagtgagctggagacagagatagaggatatcgaga
cagcctggagactgtttgagaggaaagttgaagaggatcgtcagagaagactaggagatattgagctagaagaaagtcagcgggaacagtacagggaatt
aaaggaagaagccaataaagagagtgcagtgttgaggcagcagctagatagactccagtgggagcagaaatctgcccaggagaagctggaatttgttctg
acgagacaaaaagaagtgcaggtaaacaagaaacacgttgaggaacagatagaagaacacaacaaacggatagagaaactagaggaatacataaacactt
gccttaaatctattgaagagcaaagacctcaggaggagcaactggctggggaaattaaagctagcaagcagcgaatggcagaggtcaacgagcagctgaa
cagtattgttggagagctgcaaaatgcccgtattgatttccacgaaggaagtaggcagaagaggaaagcagaggtcctggaaagcatgaaacgaatgtat
cctgacgctgtgtttggcagattatttgagctctgccacccaattcataaaaagtaccagctggcagttaccaaggtgttcggcaaatacatgaacgcca
tcatcgtatctacagtgcaggttgctagagactgcgtcaaatttcttaaagaggaaagggcggagcccgagactttcctcgccttggattatttggatat
aaaacaaatcaatgaaaagctgagagaaataaaaggcgccaagatgatggttgatgtcatacaaactgtaagcgcccctctgaagaaagtgatccagttt
gtgtgcggcaacggactggtttgtgattcggtgaaggaagcaaaacggattgcgtttgatggacctgaacgattaaaggcggtcgctctagacgggacgc
tttttttgaaatctggcgtcatatccggcggatcgagtgatctgagatataaagctaaacgctgggatgagaaggagatcaacgaactgaaagagaaaag
ggatgcgctaatggccgaattgaaagagctgattaagttacagagaagggagagcgatttaaagcaacttcaagctcagctgcaaggaacccagacgcgc
atgaagtattcacagagcgaagtggaagtcgttaaaaagaaacatctggctaattgctctatggaaaaatcccggctagacagcgagttggcgaactttg
cttctcagcttacgatgctcagagaggaactcgatgtccatggaacgaagatcggagacattcgggaacaaatgaatcaggtagaggaccgagtgtttaa
agatttctgcgaggcaatcggggtgccgaatattcgcgcgtacgaggaagaatatgtccaacagcagcaggaaatggacaagaaaagactggaatttgac
aaccagaagacaagattagggatccagctggaatacattcgggggcagctggaaaaggggaggagcaacatagacaagtcaaagcaaaccttgcagaagg
aagaaggtgataaagtcaggctgaaaaaggaagaagagcaatgtatgaaagcagtggacaaggctatggcgcagcaacaacagttcaaaaacaacttcac
ctttaagaaatccgaagttgccgacgcccagaaaaaagtcgaagaagcgagaaaggccctattgaatgtgaatagagagatgagcaaaaaaaaaaaaaaa
5' EST: EL838176 [+]gtgattatatccccggcgtcggtgtatatatagggggggctttttattagcctgttgggtagaaaggagtggctaccgggcggctgggaggggcctcgta
cctaccttactaggcctgagggaggagttagtgactgagtgatattggggggtctcagggggcaacatggggttcatgaagcagctgattattgagaatt
ttaagtcctgggggggccggcaggtcattgggcccttcctgcgattcaactgtgtcatcggccccaatggctcaggtaaatccaatctaatggacgcctt
cagcttcgtaatgggagaaaagcccgctaacctgcgagtgagaaacatccggcagcttattcacggggccaacgttggcaagcccatgtccacgacggca
agggtagtcttggtgtacagtgaggaaaatggagaggagaggcagttttctaggattatcgtaggcgactcttcagagtatcggattgatggcaagccag
tagggcgttccacatatgtaatggaactggagagaattggcatcattgttaaagccaggaactgcctagtatttcagggagaagtggaatctcttgctat
gaagaagcccagagagaggactcatctgtttgagcaaataagcaactcctgggagctagcagatgaatatgagaaaaagaagaagctgatgctgca
3' EST: EL838177 [+]tttttttttttttttgctcatctctctattcacattcaatagggcctttctcgcttcttcgacttttttctgggcgtcggcaacttcggatttcttaaag
gtgaagttgtttttgaactgttgttgctgcgccatagccttgtccactgctttcatacattgctcttcttcctttttcagcctgactttatcaccttctt
ccttctgcaaggtttgctttgacttgtctatgttgctcctccccttttccagctgcccccgaatgtattccagctggatccctaatcttgtcttctggtt
gtcaaattccagtcttttcttgtccatttcctgctgctgttggacatattcttcctcgtacgcgcgaatattcggcaccccgattgcctcgcagaaatct
ttaaacactcggtcctctacctgattcatttgttcccgaatgtctccgatcttcgttccatggacatcgagttcctctctgagcatcgtaagctgagaag
caaagttcgccaactcgctgtctagccgggatttttccatagagcaattagccagatgtttctttttaacgacttccacttcgctctgtgaatacttcat
gcgcgtctgggttccttgcagctgagcttgaagttgctttaaatcgctctcccttctctgtaacttaatcagctctttcaattcggccattagcgcatcc
cttttctctttcagttcgttgatctccttctcatcccagcgtt
Library:
Description: The library was made from dT primed cDNA and cloned into vector pCS107. PolyA RNA were primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3') and digested with NotI. cDNA was size selected using 1.1% agarose gel electrophoresis (>0.6kb) then ligated into NotI and SalI digested pCS107 vector. Primary library, non-amplified. Library constructed at the DOE Joint Genome Institute (Walnut Creek, CA) as part of the Xenopus Gene Collection project.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: