XB-CLONE-2487625
Clone Name: IMAGE:8886027
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Gene:
neu3.3
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC160978 [+]
gttgcgaaacaagtgagagcttgtgtggagtgcagcttcgtctgttcgtctgactgagtgaagactttaacttttgcggcagcaaagtacatttctgaca
aattgaacaatggaaaaacaacttggaagcaaagcgagacatttcccagtaacaacagtgttcaaacaggatcctcaaaaatctgaaggaactcaggtga
cgtacagaattccagctctaatctataacggtgaaaataaaactttccttgcctttgctgaaaaacggaaaaccactgatgatacggatgcagatgtcct
tgtaatgagaagaggaatatggagggatggtgttgtcgagtgggatatgactcatgagtgtctcagttcagcttctctaccaaaccatcgcagcatgaat
ccatgtccggtctatgaaagagagtccaaaaccctctttctgtttttcattactgtccctgttggagtctcagaacacaaacaaataaaagagaataaga
atcaggcacgcctttgttacataaccagtaaggacactggtaaaacctggagtgctgttactgatttgacagcagatgtgatcggtgagcaggagaagaa
ctgggccacctttgctgttggaccaggacatggaattcagatgaagagtgggagactgattatcccagcgtacacatattatttcacctcaggaacttca
cccacatctcatgcatttgcattctacagtgatgataaaggaagcacttggcatattggaaagtgtgtggatggtgagtctaatgaatgtcagatggctg
agatcatcgatgatgacggcaacagtaaactttactgcaatgctcgaaacaggtctggctacagaaatgaggctctaagtgaaagtaaaggaaaagattt
taagtcttcatctgtacgtaaactgacagacactggaaatggctgccaaggaagcgtgttgagttttgcccctgatcacacgtctgaaaaaacatggctg
ctatactgccatccagccgataaaagaaagagaatgagcctcggcgtatacttgaataaatgcccgagtgacttgtctggatgggggaatcctgtgattc
tgcatgagggtccgagtggatacagtgatctgactcagtgtggggatggaaaacactttgcctgtctcatggaatgtggagagaaaagtgagcttgaaag
aataggctttgtcctgtttgaacacaaagaggttatttaggtcaagggcgaatactgacagcaaaaaacaaaaaaaaaaaatctgtttttttttttttag
tttgcaaaagattaggttactactaaaaaataatggatagaaacccacaaacctgacatgaaactttacatgaaacaataaatgaactgcaacttaaaaa
aaaaaaaaaaaaa
5' EST: EL842588 [+]gttcgaccggagaagcagatgtgttgccagggtctggagtggtgggagggatgtggttccatgactggctgctgtttgtggcaaggaagatcttgattgg
ctgctgagcggtgggatccatgttggcgaaagggattaaacgaaaacattcggaggtggatgaagggatggggagcaaccctgatcctgtgttagaaggg
gagtgtccatgttcattccctgccatccagtctcactgtttaatgaacatctctctggtgaaacttcaccgtagtcttcaccatgtagagcctgatctga
ggcatcttgtgttggtggctaacacgctacgacgtcttcagggcaacttgcaggtggagccatgtgctccaggtacgtggaaaatctctgaggagtgcag
aaaagagtgttctcctgcagcgggacctgagaacaagaagccagcccttgaaaacacagaagatccacttctttccagcatggatgcctccttatattct
tccatttctaccatactcgaggacttaaataactttgatggactgagcagcccacctcttccacagattgaagatgaccagctatgtgcccctaaagtca
acccagtttcagtgagtacagaggatatgatgaaactggcatcgtccagctttttaagttcttcaccataccttttgggggacaacctagaggacatatt
tgaagacattgatacctcaatgtatgacagtgatccttgcgcgagtaataatcttttaaac
3' EST: EL842589 [+]tttttttttttttttatgaaacctttttttattttgatcataaatattcacaaaggataaatatgaatccaatccacaaattaatattagttagaaaatc
aatatattggccacaaaatggcaataaatacaatctgcagattttgaagctttcagatttaaatattgactgtaataaacagccctttaaatatcttaaa
ataccctgagccctgttacctttcagggaataatttatctgtagttaccattcagttcagaaaacttttatacatacagaatacatatttgcataaataa
ggttaaacaaggtctggcctggtgtgcagaaaacatctgtttgccagctgtacaggaggaaaaaaaagctttaacggcatgttttagtaactacaaaatt
ggttatcaggaattcatcaataaaattactccgagaatacaaagctgagaatacaaatgcaataaaacaccttttagatgtatgatctaaaactcagtac
tgccataccttcagaaaatgtagtatattatgtatgatggatacaacttttccttttacaaatctgacaggttctactagaacagctgtaattaatatgg
cttgataaccttgcattttcatacatacaattctgcatcccagatgtaccaaagatgccagttgcctgcgcgatttgtgtttgggcattctccgtttgca
agcaagaaaagtgaggttagctgtcatttttgaaacactcttcaagggcatacgtc
Library:
Description: The library was made from dT primed cDNA and cloned into vector pCS107. PolyA RNA were primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3') and digested with NotI. cDNA was size selected using 1.1% agarose gel electrophoresis (>0.6kb) then ligated into NotI and SalI digested pCS107 vector. Primary library, non-amplified. Library constructed at the DOE Joint Genome Institute (Walnut Creek, CA) as part of the Xenopus Gene Collection project.Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: