XB-CLONE-2467580
Clone Name: IMAGE:9010666
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Gene:
atp6v0a4
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC157677 [+]
CACAAAGTCTTTCCAGTAGGTCACTGAATGGATCTGCTTGATTGAGAGACACAGAGACTGCTGGGATCCCAGAGGCAACAAGATTCTTTCCAGTAGGTCA
CTGAATGGATCTGCTTGATTGAGAGACACAGAGACTGCTGGGATCCCAGGGGCAACAAGATTGATATAACTGGTGGGGGTTCGGGCTCCGCCTGCTGCGC
TCACTATGGCCTCCCTATTCCGGAGTGAAGAGATGAGCCTGACCCAGTTGATTCTGCAGGTCGAGGCCGGGTACTGCTGTATCGCGGAGTTAGGAGAGCT
GGGATTGGTGCAGTTCCGGGATCTAAATGCCAGTGTCAACAGTTTCCAGCGCCGTTTCGTGAATGAAGTGCGTCGGTGTGAAAATATGGAGAGGATCCTG
CGCTTCCTTGAGAGTGAAATGGTTAACGACAAGATCAAAATCCGGACGCCGGAGAAACTGCCGCAGACTCCGTTACCCAGAGAAATGATCGACCTGGAGA
CTGTGCTGGAGAAACTGGAAGGGGAGCTGCAGGAGGTGAACCGGAACCAGCAGGCCCTCAAGCAGAACTTCCTGGAGCTGACGGAACTCAAGCATCTGCT
GAAAAAGACTCAGGACTTTTTCGAGGCGGAGGCTAACTTGCCGGATGACTTTTTTAGCGAGGACACCAGTAGCCTGCTGGAGCTTCGAACAATCCCCAGT
GCTGCGGCTGCTGGGAAACTGGGCTTCACGGCCGGAGTCATCAACCGAGAGAGAATGGCAACATTTGAGCGGTTGCTATGGAGGGTTTGCCGGGGCAACA
TTTACCTGAAGTACACGGAATTGGACACGGCGCTGGAAGATCCGGTCACAAAAGAAGAGGTAAAGAAAAACGTATTTATTATATTCTATCAAGGAGACCA
ACTGAAACTGAAGATTAAGAAAATCTGCGATGGATTCAAGGCCACAGTGTACCCGTGCCCCGAGTCTGCCACTGAGCGCAAAGAAATGGTGGCCGATGTC
AACACAAGGATCGAAGACTTAAACACGGTGATCACCCAAACAGAGTCTCACAGGCAGCGGGTGTTGCTGGAGGCTGCCCAAAGTCTGTGCAGCTGGGGCA
TTAAGGTGAAGAAGATGAAGGCGGTGTATCACGTTCTGAACTTGTGCAACATCGACGTTACCCAGCAGTGCGTCATTGCTGAGATCTGGTGCCCTATAGC
AGACAAGGAGCAGATCAAGCAAGCCCTTCATCGTGGCATGGAGAGAAGTGGCTCCACCATTGCTCCAATTTTGACCAACATCTCTTCTAAGCTGGACCCA
CCAACCTTCAACCGCACAAACAAGTTCACAAGCGGATTTCAGAACATCGTTAATGCTTATGGAGTTGGGAGTTACAGGGAGATGAACCCAACACCCTACA
CTATCATCACGTTCCCCTTCCTCTTTGCGGTGATGTTTGGAGACTGCGGACACGGGTCTGTCATGTTGGGATTTGCCCTTTGGATGGTTCTAAATGAGAA
ACAGCTGTTAGCATCGAAGACGGATAATGAGATCTGGAACACGTTCTTTGGGGGTCGTTACCTCATCCTGCTCATGAGTATCTTCTCTATCTACACTGGA
TTCATCTATAATGACTGCTTCTCCAAATCCTTTGATATCTTTGGCTCTTCCTGGAGAGTCCGTCCTATGTTCATCAACAAAACATGGAATGATAATCTGG
TGCATCAGGGATTGCAGCTACAATTAGATCCTGCGGTTCCGGGAGTGTATTCTGGGAACCCGTATCCCTTCGGAATCGACCCAATCTGGAACATTGCACA
AAACAAGCTCACCTTCCTCAATTCGTACAAAATGAAGATGTCCGTTGTAATGGGGATCACCCAGATGGTTTTTGGGGTGATGCTTGGCCTTCTCAACCAC
ATAAACTTCAAGAGGCCCATCAACATCATCCTTCAGTTCGTTCCTGAGATGATCTTCATCATCTGCCTCTTTGGGTACCTGGTCTTCATGATCATCTTCA
AGTGGTGCAAGTATGACGTACACAGCTCGCAAGGTGCCCCGAGCATCCTCATCCACTTCATCAACATGTTCCTCTTCAACTACAGTGACCCAACCAATGC
TCCTCTCTACGAACATCAGAAAGAAGTGCAGACTTTCCTTGTTATTTTTGCCTTGATCGCCGTCCCTTGGATGCTGTTAATAAAACCCTTTGTCCTGAGG
GCAAATCACTTGAAAGCTCAACGCATGCTTCAATCATCGTCGGGGCCCGAGGATCACGCGGAACTCACAGATGTGGAAAATGCACACGCCAATCACAAGA
GTAAAAAAGAGGAGCATGGTGGGGGAGACCATGGGGGAGGACATGGAGAGCATGGTGAAGAGTTTGATTTTGGGGATATATTTGTTCACCAAGCCATCCA
TACCATAGAGTACTGCCTGGGGTGCATATCCAACACTGCCTCCTACCTGCGCCTCTGGGCTTTGAGTTTGGCCCATGCACAGCTGTCGGAGGTGCTATGG
ACCATGGTAATGCACCAAGGCCTGAGCATCGGCGGCTACGGAGGACTTATCGGCGTCTTTATTATATTCGCTGCCTTTGCCGTTCTTACTGTGGCCATCT
TGCTGGTCATGGAGGGGCTGTCGGCCTTTCTCCATGCCCTTAGGCTCCACTGGGTTGAATTCCAGAACAAATTTTACGCCGGCACGGGGCATCTGTTCAG
TCCATTTTCCTTCCAGAGTATCCTGGAGGGCACAGACAATGAGTAAAGACACCAGCACTGCCCGGCTTCCTATTTATATCCCTAGAACGAGTGCTCTTCT
GAGCGCTTTTGCAATTTATATTGTTTTTTTTTCTAGTCTTGTGATGTTGCTTTGCTTAGACCTGACCAGAGAAACTTCACATCACTGCTCACTAACTTCA
TTTTCAGCCGACTGCAGTCGCTCCAAATGGCCACAGGTGGGGCTGTTGTTTCACCAGTGTAAACAGGTCATTTCTTTCTTAGAAACAAAAATAATATAAA
GTGCAAAAGAGCTCGTTTACCTGTTTTACATTTAGTATAAAAATAAAATACAGCCAACCGTAACATAATAAAAAAAAAAATTACAAAAAAAAAAAAAAA
5' EST: EL855557 [+]cacaaagtctttccagtaggtcactgaatggatctgcttgattgagagacacagagactgctgggatcccagaggcaacaagattctttccagtaggtca
ctgaatggatctgcttgattgagagacacagagactgctgggatcccaggggcaacaagattgatataactggtgggggttcgggctccgcctgctgcgc
tcactatggcctccctattccggagtgaagagatgagcctgacccagttgattctgcaggtcgaggccgggtactgctgtatcgcggagttaggagagct
gggattggtgcagttccgggatctaaatgccagtgtcaacagtttccagcgccgtttcgtgaatgaagtgcgtcggtgtgaaaatatggagaggatcctg
cgcttccttgagagtgaaatggttaacgacaagatcaaaatccggacgccggagaaactgccgcagactccgttacccagagaaatgatcgacctggaga
ctgtgctggagaaactggaaggggagctgcaggaggtgaaccggaaccagcaggccctcaagcagaacttcctggagctgacggaactcaagcatctgct
gaaaaagactcaggactttttcgaggcggaggctaacttgccggatgacttttttagcgaggacaccagtagcctgctggagcttcgaacaatccccagt
gctgcggctgctgggaaactgggcttcacggccggagtcatcaaccgagagagaatggcaacattttgagcggttgctatggagggtttgccggggcaac
atttt
3' EST: EL855558 [+]tttttttttttttttgtaatttttttttttattatgttacggttggctgtattttatttttatactaaatgtaaaacaggtaaacgagctcttttgcact
ttatattatttttgtttctaagaaagaaatgacctgtttacactggtgaaacaacagccccacctgtggccatttggagcgactgcagtcggctgaaaat
gaagttagtgagcagtgatgtgaagtttctctggtcaggtctaagcaaagcaacatcacaagactagaaaaaaaaacaatataaattgcaaaagcgctca
gaagagcactcgttctagggatataaataggaagccgggcagtgctggtgtctttactcattgtctgtgccctccaggatactctggaaggaaaatggac
tgaacagatgccccgtgccggcgtaaaatttgttctggaattcaacccagtggagcctaagggcatggagaaaggccgacagcccctccatgaccagcaa
gatggccacagtaagaacggcaaaggcagcgaatataataaagacgccgataagtcctccgtagccgccgatgctcaggccttggtgcattaccatggtc
catagcacctccgacagctgtgcatgggccaaactcaaagcccagaggcgcaggtaggaggcagtgttggatatgcaccccaggcagtactctatggtat
ggatggcttggtgaacaaatatatccccaaaatcaaactcttcaccatgctctccatgtcctcccccatggtctcccccaccatgct
Library:
Description: The library was made from dT primed cDNA and cloned into vector pCS107. PolyA RNA were primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3') and digested with NotI. cDNA was size selected using 1.1% agarose gel electrophoresis (>0.6kb) then ligated into NotI and SalI digested pCS107 vector. Primary library, non-amplified. Library constructed at the DOE Joint Genome Institute (Walnut Creek, CA) as part of the Xenopus Gene Collection project.Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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