XB-CLONE-2456774
Clone Name: IMAGE:9015853
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Gene:
arhgef16
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC154874 [+]
AAGAAAACCCATTCATTGACAACATAAGTGATATTGTAGAACATCATGCTCAGAATAAATTCCAGCCCTACGTGAGTTACTGCTCCAATGAAATGTACCA
GCAACGGACTCTCCAAAGGCTCATGTCCACCAATGGGACATTTAAAGACACCTTAAAGCAAATCGAAATGAAGGCGGAATGCGGTGGCCTGCCCATGTTT
TCATTCCTAATTTTGCCAATGCAGAGAGTTACACGTCTTCCTCTACTGATGGATACTATTTGCCAAAAAATGGACATGCGCACTAAAGAATATGGAGAGA
CAACTCATGCCCTGAAAGCCTTAAGCAAGTTGGTTAAGCAGTGCAATGAAGGGGCACGGAAGATGGAAAGGACAGAGCAGATGTACACCCTGCAAACGCA
GCTTGAGTTCGGAAAGATCAAGCCTTTTCCACTCATTTCTGCCTCGCGCTGGTTGCTGAAGAGAGGTGAACTTAACCTTGTAGAGGAATCTGGAGTTTTC
TGGAAAGGCTTTGGAAAGACTTCTTGCTATCTCTTCTTATTCAATGACATCCTTATAATAACAAAGAAAAAAAGTGAGGAAAACTACATTGTAACAGATT
ATGTCATGATGGATCAGTTTGCTGTGGCTAAAATAGAAAATGGTGACCCACAGTTATCGCCTCCTGCAAAGACTGGGACAAGTGGCCCCCGTAGTCCAAA
TGGGCCCTTCTTGTTCCAGATTCATATCAGTAAGAACAGTGAAGGGAAACAGGATGAAATTGTGTTGGCGGCTGACAGTCTGAGTGATAGAGCACGGTGG
GTTGCTGCACTTGAGCGGAAGGAAGACAACAAAGAGAACAGCAGATCTAGGAGAGGAGGACTGCGCCAGGTGGAAATAATAAAGGCTTACTTGGCCAAAC
AAGCCGATGAAATTTCACTCCAACAAGCTGATGTGATGCTGGTGTTGCAGGAAGAAGATGGTTGGTATCAGGGAGAAAGACTACGAGACGGAGAAAGAGG
TTGGTTTCCTGAGTCATGTGCTAAGGAAATTACCAACCAAACAGCCGTAGAAAGGAATGTTCAGCGAATGGAGAGACTGAGGATTGAGACGGATGTGTAA
TGGAAGAGATGGACTTTCCAAGCCAAGGCTGCACTCTGAGACAGACTTGTGAATCCATGCAATGCACAGCTCTTGGACCGGTGGTTGGTCAACTTCGTTT
GGATCATAAAATACATTTCAAGGGATCTTCTTCACCTTTTCCAAAGGGAAGTTTGTTCTACTGCATTGAGGCCAATCGCAGTGGATGTTATTAGGTTGCC
AGAAGACCCATGGGAACATGACATTTAAATTTGGACCATCTTGGTCACCCTGCCCTGGCTTATATTACCCAGGGAGCAACACTGCATGCTCCAAACTGGC
AGGACATTTTCAGTATGATTCAAACAGCTGTGACATATTCAATGAAGCAGTGTGTTGTTGGGGTAACTCAATGCCAAGTTGGGGAAGAGCAGAATAGTGA
GGAATGAAAGCCGCCCAAAATATTTTCTTATTGCCTTCCCCTCGTATAAATCAAGTGTAAAAATGAAGAGAAACAAGCTGCAGGCTTTAGTTTTGCTTGT
ATAGAGGTGTAACACTGTGCAGGCATGGAATCTGGAACCCTGTTGCTACAAAATTTACCACTTCCATTTTCTGTAGTCTCCTATTTGAACCAAAAGTTCC
TCACCTTCAGTTGATTTGATTTTTCTCTACGCTGGCATAAATCCATGTTTGTAATAAAGAAATGCTATTGGTTTCTTCTTTTATTGTTTAGGCATAGCGC
CCCATGTTACTGACCCATTATCCAGGAACATTCAGATCCCATGCATTCCAGATAATAGATCCCATAACTGTATCCACTCTCAGAAACACATGCAAACCAA
TAACATTGAGTTGCCAAACACCACATGTTCTTTGTCCTAAATATAATCAAAAATGGTGTCTTGCCAATAGGAATTGATGTTCCAAATCCTCACTGTTCTT
TCCCAAAATAATCTATGGGGCTATTACTCTGATTTCCCTTTCTGGGGCAAAATGTTGATAGGAATTCTGCATTTTCATGGACAGTTATTGCTTTATTATT
GCAGGAATTCTGTCAGATCTACATAGGGCTTTATTAACATTGGAGACAAACATTACATTATTGCCCATAGCAATCAGATGTTTGATTTTGTTTTCTAGCT
TGTAGACTAGCATTCAAATCTAATTGCTGATTGGCTGCTATGGGCAACATCACCAGTGATGTTTGTCTCCATTGTTAATAAATACACCCCTACAAATCTG
CATTTTGGTGCTTTACTGTATCTGCTGCACTCTTTTTCTAGGAAAGTATATACGCGAGCCTTTGTTATGGGTATTTTGTACATATTCTACACCATAACCA
TTTTTTAGTAAATAAGTGAAATGAATGTATTTGGGGGAAATGCTGTATATAGCTACAGTGATGCCAAAATGATTTGGTCTCTTTTAGAATAAAGAGAGAA
AAAAAAGTCCAGAGATTTTTTTTTACATGTATGTTAACTGAAATATTATAGCAATATTAAAGTGGCAGTTTACCTTAAATTAACGTTAAATATGATGAAG
ACATTGATATTCTTGGGCAATAAATTTCTTTATTTTTAAAAAAAAAAAAAAA
5' EST: EL862532 [+]aagaaaacccattcattgacaacataagtgatattgtagaacatcatgctcagaataaattccagccctacgtgagttactgctccaatgaaatgtacca
gcaacggactctccaaaggctcatgtccaccaatgggacatttaaagacaccttaaagcaaatcgaaatgaaggcggaatgcggtggcctgcccatgttt
tcattcctaattttgccaatgcagagagttacacgtcttcctctactgatggatactatttgccaaaaaatggacatgcgcactaaagaatatggagaga
caactcatgccctgaaagccttaagcaagttggttaagcagtgcaatgaaggggcacggaagatggaaaggacagagcagatgtacaccctgcaaacgca
gcttgagttcggaaagatcaagccttttccactcatttctgcctcgcgctggttgctgaagagaggtgaacttaaccttgtagaggaatctggagttttc
tggaaaggctttggaaagacttcttgctatctcttcttattcaatgacatccttataataacaaagaaaaaaagtgaggaaaactacattgtaacagatt
atgtcatgatggatcagtttgctgtggctaaaatagaaaatggtgacccacagttatcgcctcctgcaaagactgggacaagtggcccccgtagtccaaa
tgggcccttcttgt
3' EST: EL862533 [+]tttttttttttttttaaaaataaagaaatttattgcccaagaatatcaatgtcttcatcatatttaacgttaatttaaggtaaactgccactttaatatt
gctataatatttcagttaacatacatgtaaaaaaaaatctctggacttttttttctctctttattctaaaagagaccaaatcattttggcatcactgtag
ctatatacagcatttcccccaaatacattcatttcacttatttactaaaaaatggttatggtgtagaatatgtacaaaatacccataacaaaggctcgcg
tatatactttcctagaaaaagagtgcagcagatacagtaaagcaccaaaatgcagatttgtaggggtgtatttattaacaatggagacaaacatcactgg
tgatgttgcccatagcagccaatcagcaattagatttgaatgctagtctacaagctagaaaacaaaatcaaacatctgattgctatgggcaataatgtaa
tgtttgtctccaatgttaataaagccctatgtagatctgacagaattcctgcaataataaagcaataactgtccatgaaaatgcagaattcctatcaaca
ttttgccccagaaagggaaatcagagtaatagccccatagattattttgggaaagaacagtgaggatttggaacatcaattcctattggcaagacaccat
ttttgattatatttaggacaaagaa
Library:
Description: The library was made from dT primed cDNA and cloned into vector pCS107. PolyA RNA were primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3') and digested with NotI. cDNA was size selected using 1.1% agarose gel electrophoresis (>0.6kb) then ligated into NotI and SalI digested pCS107 vector. Primary library, non-amplified. Library constructed at the DOE Joint Genome Institute (Walnut Creek, CA) as part of the Xenopus Gene Collection project.Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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