XB-CLONE-2455215
Clone Name: IMAGE:9016747
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Gene:
aven
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC153755 [+]
AGGAACGGAAAGTAATTGGCAGCTGCACCGAACACATGGAGCGGGGCCGCGGGAGACACCGACGCGGAGGTGGCGGTCGTAGGAGGCCCTGGGGTGAACG
GGGACGCGGAGGGGACAGGGTAGGACCCGCACACAGTGGCAGGGGATACAGGGGCGCAGACCGCCAGCGCAATGAGCCGTCAGAACCTAGAATTGAGGAC
AGGAATGAGGCTGTAGAACCCCAGGAAGAGGATGAACAGGTGTCGCTGGGATTCTCCAGGAGGAAAATCAGTTCCAACTGGGATCGCTATGAAGAAACTG
AGAAGGAGTCTGAGTCTGAAAGCAAAGTGCTTCAAAGAGGAGCCGATTACAGTGTCCTCCTTAGTTCAGCAGGGGATTCCTTCACTCAGTTTAGATTTGC
TGATGAGAAAGACTGGGAAGCAGAAACTCCAAGCTACAAACAGGCCTCCGCTGTGTGTTTGGATCCCCAGTCTCTAGTGCGAGTCCTGCAGGAGCTGCCG
CTGCATCTGCGCCTCAACGTGGAGCCGGAGCTGGTTCAGGAAGTGTTACCACAGGAACTGCCTCATTTTAAAACGAGAGACATTTTAATCACGCCTCCTG
TCATCCAAGCCCCGCCCAGTACTGGCGCAGCAAGTTCTACTCAGGTGGGAATGGCTCCTCCAGAAGAACAGGTTTCAAATACCACAAGGGCCCAGGACAG
CTCCCCTCAGCTTGAATTAGATGAAGAACTTGACTTTCTACTTAGTCTGGAAGCCCCAGTGAAAGAGGCAGCAGGCGTCAAACCCACCAATGAAGAGAAG
GAGTGTGACCTTCATGGGAAGCGTCAAGAACTTCTTCCAATAACAGATGAAGTTGCCTCCTCTTCCGCTGAGAAGAATAAATCTGTAACTGAAGAAGATC
TAGAGGACTGGTTGGACAGCATGATCTCCTGATGTGCCGGGGGCAGGGAAGCTAAAGGGAGCGTATATACATATATAGAGCAGTGGCTCCTTTCTATAGA
CCTGCCTCCTAACTGCCTGTTTCTTGTACCTTTATATATCCTGTATAATAGAATGAAAACGCTAGTGAATATTAGTTTTATGCAGTAACTATATTATTCC
AAACATGGTCAGAATTAGGGATTGCATTTGAACTGCTTAATGTTGGTCTTTTTCAGTTAAGCAGAAAATGCTGTTGAAGGCCCATGCCCTTGGGTCACAC
AGTTCTCACCCAGCAAATGGCTTATTCCAATTGTCACCGTATGGTTTACCATTATTACCAAAAATAAAACATTGCCAAGCAGTTACACAAAAAAAAAAAA
AAA
5' EST: EL863533 [+]aggaacggaaagtaattggcagctgcaccgaacacatggagcggggccgcgggagacaccgacgcggaggtggcggtcgtaggaggccctggggtgaacg
gggacgcggaggggacagggtaggacccgcacacagtggcaggggatacaggggcgcagaccgccagcgcaatgagccgtcagaacctagaattgaggac
aggaatgaggctgtagaaccccaggaagaggatgaacaggtgtcgctgggattctccaggaggaaaatcagttccaactgggatcgctatgaagaaactg
agaaggagtctgagtctgaaagcaaagtgcttcaaagaggagccgattacagtgtcctccttagttcagcaggggattccttcactcagtttagatttgc
tgatgagaaagactgggaagcagaaactccaagctacaaacaggcctccgctgtgtgtttggatccccagtctctagtgcgagtcctgcaggagctgccg
ctgcatctgcgcctcaacgtggagccggagctggttcaggaagtgttaccacaggaactgcctcattttaaaacgagagacattttaatcacgcctcctg
tcatccaagccccgcccagtactggcgcagcaagttctactcaggtgggaatggctcctccagaagaacaggtttcaaataccacaagggcccaggacag
ctcccctcagcttgaattagatgaagaacttgactttctactta
3' EST: EL863534 [+]tttttttttttttttgtgtaactgcttggcaatgttttatttttggtaataatggtaaaccatacggtgacaattggaataagccatttgctgggtgaga
actgtgtgacccaagggcatgggccttcaacagcattttctgcttaactgaaaaagaccaacattaagcagttcaaatgcaatccctaattctgaccatg
tttggaataatatagttactgcataaaactaatattcactagcgttttcattctattatacaggatatataaaggtacaagaaacaggcagttaggaggc
aggtctatagaaaggagccactgctctatatatgtatatacgctccctttagcttccctgcccccggcacatcaggagatcatgctgtccaaccagtcct
ctagatcttcttcagttacagatttattcttctcagcggaagaggaggcaacttcatctgttattggaagaagttcttgacgcttcccatgaaggtcaca
ctccttctcttcattggtgggtttgacgcctgctgcctctttcactggggcttccagactaagtagaaagtcaagttcttcatctaattcaagctgaggg
gagctgtcctgggcccttgtggtatttgaaacctgttcttctggaggagccattcccacctgagtagaacttgctgcgccagtactgggcggggcttgga
tgacaggaggcgtgattaaaatgtctctcgttttaaaatgaggcagttcctgtggtaacacttcctgaaccagctccggct
Library:
Description: The library was made from dT primed cDNA and cloned into vector pCS107. PolyA RNA were primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3') and digested with NotI. cDNA was size selected using 1.1% agarose gel electrophoresis (>0.6kb) then ligated into NotI and SalI digested pCS107 vector. Primary library, non-amplified. Library constructed at the DOE Joint Genome Institute (Walnut Creek, CA) as part of the Xenopus Gene Collection project.Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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