XB-CLONE-2448778
Clone Name: IMAGE:9018802
|
Gene:
ripply2.2
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: EL867703 [+]
aagaagcgtgcggagacaatcacttgggaatggagaacatcatgtctatggaccttggtggggcatctggtggcttgctaacgccgagtggctccagcta
caagcgcctcaggaactgcaagtccttacctgagagatggaagtccagaggagctgtcaggacagatccacagcgaactcacacattgttctggagacct
tggcttgtcaaatcccataaaccaaagacccaaatccatccatatgccagaggcttgtgtgaaaatcctcaagttcagaagcctgtggagtataaccatc
ctgtgagattattttggcccaaatcaaagttattggctcacacctaccaagaggcagcagatttgcttagaaactttcctgttcaagccacgatatctct
ctacaatgactccgagtcagacacagacaatgaagaggacagcagtgaggaggaaaatgactctggctttgaatcagaataatagcccatcacactattt
ctcagaagaaccatgggactggaaattaagagacttgcagccacttgtgtatatctgtacatatgtacacaggtaaacatgaagcttgcagaatgtcact
gtaattaatacagttttgattctgaccctcaaactgttggtccccaggtcagaaagcactggtttaacctgatatttgtatggcattgttacactttaat
gcttttggggtccagtgcaaaatgaacttttttggggtcacataacttaaaagtaggactgtcatattcatgggcctttagcattgctaaacaacagttt
tcagcaatcaccaatgagaaaagggctgt
3' EST: EL867704 [+]tttttttttttttttatgtttaacattacagctttattaataagtcatgccatatcatacaaataaatatatttcacttatatataatatcttacatttt
tacagtatatattaaagcggtgctttacattttcccacataccagttggagaatgatgctcacccatcttgatacaagagcagacatacgcttgctgatt
tcagtcttttaaaagaaaagtgctctggttgagaacctttcctgttttaagcccccactcatactgtatgagtggccctacacatgtatggtcacttttc
tatttgttcattattatgacatttctctatgcagctattctcaactagttagcgttacacacaaatgctatatgatgcccaaattaattgaacatatatt
catggaaattataatatgtatgtgaaattataatatgtaaccatatataacaataacttatataaccaatgtagtgtggaaaaaaaagaaaacctaatat
ttcctgtcatataattccagtgcatcagtttaaaattgaccttcttagattaaattgttatgttttgaacctaaccaagcctggtacattcatcactacc
atatatgaaagaaaagtatttttactttcttcatagtctatttcactaaccatagcttattttatagtgcttttttttaaattgccacttttcacggtaa
agtaaatacagctaggcaaaatattctcttctgttactcattcagttcatgttttcatacatatacatgtatattcagagttgtgctcagggggttccca
agcagggagtacctgcactgagcctt
Library:
Description: The library was made from dT primed cDNA and cloned into vector pCS107. PolyA RNA were primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3') and digested with NotI. cDNA was size selected using 1.1% agarose gel electrophoresis (>0.6kb) then ligated into NotI and SalI digested pCS107 vector. Primary library, non-amplified. Library constructed at the DOE Joint Genome Institute (Walnut Creek, CA) as part of the Xenopus Gene Collection project.Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: